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Fine mapping of the gene for susceptibility to black spot disease in Japanese pear (Pyrus pyrifolia Nakai)
Black spot disease, which is caused by the Japanese pear pathotype of the filamentous fungus Alternaria alternata (Fries) Keissler, is one of the most harmful diseases in Japanese pear cultivation. We mapped a gene for susceptibility to black spot disease in the Japanese pear (Pyrus pyrifolia Nakai)...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Society of Breeding
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4785004/ https://www.ncbi.nlm.nih.gov/pubmed/27162498 http://dx.doi.org/10.1270/jsbbs.66.271 |
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author | Terakami, Shingo Moriya, Shigeki Adachi, Yoshihiko Kunihisa, Miyuki Nishitani, Chikako Saito, Toshihiro Abe, Kazuyuki Yamamoto, Toshiya |
author_facet | Terakami, Shingo Moriya, Shigeki Adachi, Yoshihiko Kunihisa, Miyuki Nishitani, Chikako Saito, Toshihiro Abe, Kazuyuki Yamamoto, Toshiya |
author_sort | Terakami, Shingo |
collection | PubMed |
description | Black spot disease, which is caused by the Japanese pear pathotype of the filamentous fungus Alternaria alternata (Fries) Keissler, is one of the most harmful diseases in Japanese pear cultivation. We mapped a gene for susceptibility to black spot disease in the Japanese pear (Pyrus pyrifolia Nakai) cultivar ‘Kinchaku’ (Aki gene) at the top of linkage group 11, similar to the positions of the susceptibility genes Ani in ‘Osa Nijisseiki’ and Ana in ‘Nansui’. Using synteny-based marker enrichment, we developed novel apple SSR markers in the target region. We constructed a fine map of linkage group 11 of ‘Kinchaku’ and localized the Aki locus within a 1.5-cM genome region between SSR markers Mdo.chr11.28 and Mdo.chr11.34. Marker Mdo.chr11.30 co-segregated with Aki in all 621 F(1) plantlets of a ‘Housui’ × ‘Kinchaku’ cross. The physical size of the Aki region, which includes three markers (Mdo.chr11.28, Mdo.chr11.30, and Mdo.chr11.34), was estimated to be 250 Kb in the ‘Golden Delicious’ apple genome and 107 Kb in the ‘Dangshansuli’ Chinese pear genome. Our results will help to identify the candidate gene for susceptibility to black spot disease in Japanese pear. |
format | Online Article Text |
id | pubmed-4785004 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Japanese Society of Breeding |
record_format | MEDLINE/PubMed |
spelling | pubmed-47850042016-05-09 Fine mapping of the gene for susceptibility to black spot disease in Japanese pear (Pyrus pyrifolia Nakai) Terakami, Shingo Moriya, Shigeki Adachi, Yoshihiko Kunihisa, Miyuki Nishitani, Chikako Saito, Toshihiro Abe, Kazuyuki Yamamoto, Toshiya Breed Sci Research Paper Black spot disease, which is caused by the Japanese pear pathotype of the filamentous fungus Alternaria alternata (Fries) Keissler, is one of the most harmful diseases in Japanese pear cultivation. We mapped a gene for susceptibility to black spot disease in the Japanese pear (Pyrus pyrifolia Nakai) cultivar ‘Kinchaku’ (Aki gene) at the top of linkage group 11, similar to the positions of the susceptibility genes Ani in ‘Osa Nijisseiki’ and Ana in ‘Nansui’. Using synteny-based marker enrichment, we developed novel apple SSR markers in the target region. We constructed a fine map of linkage group 11 of ‘Kinchaku’ and localized the Aki locus within a 1.5-cM genome region between SSR markers Mdo.chr11.28 and Mdo.chr11.34. Marker Mdo.chr11.30 co-segregated with Aki in all 621 F(1) plantlets of a ‘Housui’ × ‘Kinchaku’ cross. The physical size of the Aki region, which includes three markers (Mdo.chr11.28, Mdo.chr11.30, and Mdo.chr11.34), was estimated to be 250 Kb in the ‘Golden Delicious’ apple genome and 107 Kb in the ‘Dangshansuli’ Chinese pear genome. Our results will help to identify the candidate gene for susceptibility to black spot disease in Japanese pear. Japanese Society of Breeding 2016-03 2016-03-01 /pmc/articles/PMC4785004/ /pubmed/27162498 http://dx.doi.org/10.1270/jsbbs.66.271 Text en Copyright © 2016 by JAPANESE SOCIETY OF BREEDING http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Terakami, Shingo Moriya, Shigeki Adachi, Yoshihiko Kunihisa, Miyuki Nishitani, Chikako Saito, Toshihiro Abe, Kazuyuki Yamamoto, Toshiya Fine mapping of the gene for susceptibility to black spot disease in Japanese pear (Pyrus pyrifolia Nakai) |
title | Fine mapping of the gene for susceptibility to black spot disease in Japanese pear (Pyrus pyrifolia Nakai) |
title_full | Fine mapping of the gene for susceptibility to black spot disease in Japanese pear (Pyrus pyrifolia Nakai) |
title_fullStr | Fine mapping of the gene for susceptibility to black spot disease in Japanese pear (Pyrus pyrifolia Nakai) |
title_full_unstemmed | Fine mapping of the gene for susceptibility to black spot disease in Japanese pear (Pyrus pyrifolia Nakai) |
title_short | Fine mapping of the gene for susceptibility to black spot disease in Japanese pear (Pyrus pyrifolia Nakai) |
title_sort | fine mapping of the gene for susceptibility to black spot disease in japanese pear (pyrus pyrifolia nakai) |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4785004/ https://www.ncbi.nlm.nih.gov/pubmed/27162498 http://dx.doi.org/10.1270/jsbbs.66.271 |
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