Improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a synthetic-peptide 24-mer repeat sequence of glycoprotein G as an antigen

To increase the sensitivity of an enzyme-linked immunosorbent assay (ELISA) for equine herpesvirus type 4 (EHV-4) that uses a 12-mer peptide of glycoprotein G (gG4-12-mer: MKNNPIYSEGSL) [4], we used a longer peptide consisting of a 24-mer repeat sequence (gG4-24-mer: MKNNPIYSEGSLMLNVQHDDSIHT) as an...

Descripción completa

Detalles Bibliográficos
Autores principales: BANNAI, Hiroshi, NEMOTO, Manabu, TSUJIMURA, Koji, YAMANAKA, Takashi, MAEDA, Ken, KONDO, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2015
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4785124/
https://www.ncbi.nlm.nih.gov/pubmed/26424485
http://dx.doi.org/10.1292/jvms.15-0275
_version_ 1782420348396896256
author BANNAI, Hiroshi
NEMOTO, Manabu
TSUJIMURA, Koji
YAMANAKA, Takashi
MAEDA, Ken
KONDO, Takashi
author_facet BANNAI, Hiroshi
NEMOTO, Manabu
TSUJIMURA, Koji
YAMANAKA, Takashi
MAEDA, Ken
KONDO, Takashi
author_sort BANNAI, Hiroshi
collection PubMed
description To increase the sensitivity of an enzyme-linked immunosorbent assay (ELISA) for equine herpesvirus type 4 (EHV-4) that uses a 12-mer peptide of glycoprotein G (gG4-12-mer: MKNNPIYSEGSL) [4], we used a longer peptide consisting of a 24-mer repeat sequence (gG4-24-mer: MKNNPIYSEGSLMLNVQHDDSIHT) as an antigen. Sera of horses experimentally infected with EHV-4 reacted much more strongly to the gG4-24-mer peptide than to the gG4-12-mer peptide. We used peptide ELISAs to test paired sera from horses naturally infected with EHV-4 (n=40). gG4-24-mer ELISA detected 37 positive samples (92.5%), whereas gG4-12-mer ELISA detected only 28 (70.0%). gG4-24-mer ELISA was much more sensitive than gG4-12-mer ELISA.
format Online
Article
Text
id pubmed-4785124
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher The Japanese Society of Veterinary Science
record_format MEDLINE/PubMed
spelling pubmed-47851242016-03-10 Improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a synthetic-peptide 24-mer repeat sequence of glycoprotein G as an antigen BANNAI, Hiroshi NEMOTO, Manabu TSUJIMURA, Koji YAMANAKA, Takashi MAEDA, Ken KONDO, Takashi J Vet Med Sci Virology To increase the sensitivity of an enzyme-linked immunosorbent assay (ELISA) for equine herpesvirus type 4 (EHV-4) that uses a 12-mer peptide of glycoprotein G (gG4-12-mer: MKNNPIYSEGSL) [4], we used a longer peptide consisting of a 24-mer repeat sequence (gG4-24-mer: MKNNPIYSEGSLMLNVQHDDSIHT) as an antigen. Sera of horses experimentally infected with EHV-4 reacted much more strongly to the gG4-24-mer peptide than to the gG4-12-mer peptide. We used peptide ELISAs to test paired sera from horses naturally infected with EHV-4 (n=40). gG4-24-mer ELISA detected 37 positive samples (92.5%), whereas gG4-12-mer ELISA detected only 28 (70.0%). gG4-24-mer ELISA was much more sensitive than gG4-12-mer ELISA. The Japanese Society of Veterinary Science 2015-10-01 2016-02 /pmc/articles/PMC4785124/ /pubmed/26424485 http://dx.doi.org/10.1292/jvms.15-0275 Text en ©2016 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Virology
BANNAI, Hiroshi
NEMOTO, Manabu
TSUJIMURA, Koji
YAMANAKA, Takashi
MAEDA, Ken
KONDO, Takashi
Improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a synthetic-peptide 24-mer repeat sequence of glycoprotein G as an antigen
title Improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a synthetic-peptide 24-mer repeat sequence of glycoprotein G as an antigen
title_full Improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a synthetic-peptide 24-mer repeat sequence of glycoprotein G as an antigen
title_fullStr Improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a synthetic-peptide 24-mer repeat sequence of glycoprotein G as an antigen
title_full_unstemmed Improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a synthetic-peptide 24-mer repeat sequence of glycoprotein G as an antigen
title_short Improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a synthetic-peptide 24-mer repeat sequence of glycoprotein G as an antigen
title_sort improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a synthetic-peptide 24-mer repeat sequence of glycoprotein g as an antigen
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4785124/
https://www.ncbi.nlm.nih.gov/pubmed/26424485
http://dx.doi.org/10.1292/jvms.15-0275
work_keys_str_mv AT bannaihiroshi improvementofanenzymelinkedimmunosorbentassayforequineherpesvirustype4byusingasyntheticpeptide24merrepeatsequenceofglycoproteingasanantigen
AT nemotomanabu improvementofanenzymelinkedimmunosorbentassayforequineherpesvirustype4byusingasyntheticpeptide24merrepeatsequenceofglycoproteingasanantigen
AT tsujimurakoji improvementofanenzymelinkedimmunosorbentassayforequineherpesvirustype4byusingasyntheticpeptide24merrepeatsequenceofglycoproteingasanantigen
AT yamanakatakashi improvementofanenzymelinkedimmunosorbentassayforequineherpesvirustype4byusingasyntheticpeptide24merrepeatsequenceofglycoproteingasanantigen
AT maedaken improvementofanenzymelinkedimmunosorbentassayforequineherpesvirustype4byusingasyntheticpeptide24merrepeatsequenceofglycoproteingasanantigen
AT kondotakashi improvementofanenzymelinkedimmunosorbentassayforequineherpesvirustype4byusingasyntheticpeptide24merrepeatsequenceofglycoproteingasanantigen

Ejemplares similares