Intracellular nanoparticles mass quantification by near-edge absorption soft X-ray nanotomography

We used soft X-ray three-dimensional imaging to quantify the mass of superparamagnetic iron oxide nanoparticles (SPION) within whole cells, by exploiting the iron oxide differential absorption contrast. Near-edge absorption soft X-ray nanotomography (NEASXT) combines whole-cell 3D structure determin...

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Detalles Bibliográficos
Autores principales: Conesa, Jose Javier, Otón, Joaquín, Chiappi, Michele, Carazo, Jose María, Pereiro, Eva, Chichón, Francisco Javier, Carrascosa, José L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4785355/
https://www.ncbi.nlm.nih.gov/pubmed/26960695
http://dx.doi.org/10.1038/srep22354
Descripción
Sumario:We used soft X-ray three-dimensional imaging to quantify the mass of superparamagnetic iron oxide nanoparticles (SPION) within whole cells, by exploiting the iron oxide differential absorption contrast. Near-edge absorption soft X-ray nanotomography (NEASXT) combines whole-cell 3D structure determination at 50 nm resolution, with 3D elemental mapping and high throughput. We detected three-dimensional distribution of SPIONs within cells with 0.3 g/cm(3) sensitivity, sufficient for detecting the density corresponding to a single nanoparticle.

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