Intracellular nanoparticles mass quantification by near-edge absorption soft X-ray nanotomography

We used soft X-ray three-dimensional imaging to quantify the mass of superparamagnetic iron oxide nanoparticles (SPION) within whole cells, by exploiting the iron oxide differential absorption contrast. Near-edge absorption soft X-ray nanotomography (NEASXT) combines whole-cell 3D structure determin...

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Detalles Bibliográficos
Autores principales: Conesa, Jose Javier, Otón, Joaquín, Chiappi, Michele, Carazo, Jose María, Pereiro, Eva, Chichón, Francisco Javier, Carrascosa, José L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4785355/
https://www.ncbi.nlm.nih.gov/pubmed/26960695
http://dx.doi.org/10.1038/srep22354
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author Conesa, Jose Javier
Otón, Joaquín
Chiappi, Michele
Carazo, Jose María
Pereiro, Eva
Chichón, Francisco Javier
Carrascosa, José L.
author_facet Conesa, Jose Javier
Otón, Joaquín
Chiappi, Michele
Carazo, Jose María
Pereiro, Eva
Chichón, Francisco Javier
Carrascosa, José L.
author_sort Conesa, Jose Javier
collection PubMed
description We used soft X-ray three-dimensional imaging to quantify the mass of superparamagnetic iron oxide nanoparticles (SPION) within whole cells, by exploiting the iron oxide differential absorption contrast. Near-edge absorption soft X-ray nanotomography (NEASXT) combines whole-cell 3D structure determination at 50 nm resolution, with 3D elemental mapping and high throughput. We detected three-dimensional distribution of SPIONs within cells with 0.3 g/cm(3) sensitivity, sufficient for detecting the density corresponding to a single nanoparticle.
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spelling pubmed-47853552016-03-11 Intracellular nanoparticles mass quantification by near-edge absorption soft X-ray nanotomography Conesa, Jose Javier Otón, Joaquín Chiappi, Michele Carazo, Jose María Pereiro, Eva Chichón, Francisco Javier Carrascosa, José L. Sci Rep Article We used soft X-ray three-dimensional imaging to quantify the mass of superparamagnetic iron oxide nanoparticles (SPION) within whole cells, by exploiting the iron oxide differential absorption contrast. Near-edge absorption soft X-ray nanotomography (NEASXT) combines whole-cell 3D structure determination at 50 nm resolution, with 3D elemental mapping and high throughput. We detected three-dimensional distribution of SPIONs within cells with 0.3 g/cm(3) sensitivity, sufficient for detecting the density corresponding to a single nanoparticle. Nature Publishing Group 2016-03-10 /pmc/articles/PMC4785355/ /pubmed/26960695 http://dx.doi.org/10.1038/srep22354 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Conesa, Jose Javier
Otón, Joaquín
Chiappi, Michele
Carazo, Jose María
Pereiro, Eva
Chichón, Francisco Javier
Carrascosa, José L.
Intracellular nanoparticles mass quantification by near-edge absorption soft X-ray nanotomography
title Intracellular nanoparticles mass quantification by near-edge absorption soft X-ray nanotomography
title_full Intracellular nanoparticles mass quantification by near-edge absorption soft X-ray nanotomography
title_fullStr Intracellular nanoparticles mass quantification by near-edge absorption soft X-ray nanotomography
title_full_unstemmed Intracellular nanoparticles mass quantification by near-edge absorption soft X-ray nanotomography
title_short Intracellular nanoparticles mass quantification by near-edge absorption soft X-ray nanotomography
title_sort intracellular nanoparticles mass quantification by near-edge absorption soft x-ray nanotomography
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4785355/
https://www.ncbi.nlm.nih.gov/pubmed/26960695
http://dx.doi.org/10.1038/srep22354
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