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Directed surface attachment of nanomaterials via coiled-coil-driven self-assembly

Numerous nanoscale devices and materials have been fabricated in recent years using a variety of biological scaffolds. However, the interfacing of these devices and materials into existing circuits and ordered arrays has proved problematic. Here, we describe a simple solution to this problem using s...

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Autores principales: White, Simon J, Johnson, Steven, Szymonik, Michal, Wardingley, Richard A, Pye, Douglas, Davies, A Giles, Wälti, Christoph, Stockley, Peter G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4785676/
https://www.ncbi.nlm.nih.gov/pubmed/23154792
http://dx.doi.org/10.1088/0957-4484/23/49/495304
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author White, Simon J
Johnson, Steven
Szymonik, Michal
Wardingley, Richard A
Pye, Douglas
Davies, A Giles
Wälti, Christoph
Stockley, Peter G
author_facet White, Simon J
Johnson, Steven
Szymonik, Michal
Wardingley, Richard A
Pye, Douglas
Davies, A Giles
Wälti, Christoph
Stockley, Peter G
author_sort White, Simon J
collection PubMed
description Numerous nanoscale devices and materials have been fabricated in recent years using a variety of biological scaffolds. However, the interfacing of these devices and materials into existing circuits and ordered arrays has proved problematic. Here, we describe a simple solution to this problem using self-assembly of the peptide coiled-coil heterodimer ACID:BASE to immobilize M13 bacteriophage particles to specific locations on a patterned gold surface. Surface plasmon resonance demonstrated that free ACID peptides will assemble onto a surface derivatized with BASE. We then displayed the ACID peptide on the pIX coat protein of M13 and showed that these phage particles permit formation of the coiled-coil resulting in specific surface attachment. The ACID:immobilized BASE affinities appear to be similar for free peptide and phage-displayed ACID. Finally, we fabricated two gold electrodes, separated by a 200 nm gap, coated one of them with BASE and showed that this allows localization of the M13:ACID onto the functionalized electrode.
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spelling pubmed-47856762016-03-10 Directed surface attachment of nanomaterials via coiled-coil-driven self-assembly White, Simon J Johnson, Steven Szymonik, Michal Wardingley, Richard A Pye, Douglas Davies, A Giles Wälti, Christoph Stockley, Peter G Nanotechnology Article Numerous nanoscale devices and materials have been fabricated in recent years using a variety of biological scaffolds. However, the interfacing of these devices and materials into existing circuits and ordered arrays has proved problematic. Here, we describe a simple solution to this problem using self-assembly of the peptide coiled-coil heterodimer ACID:BASE to immobilize M13 bacteriophage particles to specific locations on a patterned gold surface. Surface plasmon resonance demonstrated that free ACID peptides will assemble onto a surface derivatized with BASE. We then displayed the ACID peptide on the pIX coat protein of M13 and showed that these phage particles permit formation of the coiled-coil resulting in specific surface attachment. The ACID:immobilized BASE affinities appear to be similar for free peptide and phage-displayed ACID. Finally, we fabricated two gold electrodes, separated by a 200 nm gap, coated one of them with BASE and showed that this allows localization of the M13:ACID onto the functionalized electrode. 2012-11-16 2012-12-14 /pmc/articles/PMC4785676/ /pubmed/23154792 http://dx.doi.org/10.1088/0957-4484/23/49/495304 Text en Content from this work may be used under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 licence (http://creativecommons.org/licenses/by-nc-sa/3.0/) . Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
spellingShingle Article
White, Simon J
Johnson, Steven
Szymonik, Michal
Wardingley, Richard A
Pye, Douglas
Davies, A Giles
Wälti, Christoph
Stockley, Peter G
Directed surface attachment of nanomaterials via coiled-coil-driven self-assembly
title Directed surface attachment of nanomaterials via coiled-coil-driven self-assembly
title_full Directed surface attachment of nanomaterials via coiled-coil-driven self-assembly
title_fullStr Directed surface attachment of nanomaterials via coiled-coil-driven self-assembly
title_full_unstemmed Directed surface attachment of nanomaterials via coiled-coil-driven self-assembly
title_short Directed surface attachment of nanomaterials via coiled-coil-driven self-assembly
title_sort directed surface attachment of nanomaterials via coiled-coil-driven self-assembly
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4785676/
https://www.ncbi.nlm.nih.gov/pubmed/23154792
http://dx.doi.org/10.1088/0957-4484/23/49/495304
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