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Directed surface attachment of nanomaterials via coiled-coil-driven self-assembly
Numerous nanoscale devices and materials have been fabricated in recent years using a variety of biological scaffolds. However, the interfacing of these devices and materials into existing circuits and ordered arrays has proved problematic. Here, we describe a simple solution to this problem using s...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4785676/ https://www.ncbi.nlm.nih.gov/pubmed/23154792 http://dx.doi.org/10.1088/0957-4484/23/49/495304 |
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author | White, Simon J Johnson, Steven Szymonik, Michal Wardingley, Richard A Pye, Douglas Davies, A Giles Wälti, Christoph Stockley, Peter G |
author_facet | White, Simon J Johnson, Steven Szymonik, Michal Wardingley, Richard A Pye, Douglas Davies, A Giles Wälti, Christoph Stockley, Peter G |
author_sort | White, Simon J |
collection | PubMed |
description | Numerous nanoscale devices and materials have been fabricated in recent years using a variety of biological scaffolds. However, the interfacing of these devices and materials into existing circuits and ordered arrays has proved problematic. Here, we describe a simple solution to this problem using self-assembly of the peptide coiled-coil heterodimer ACID:BASE to immobilize M13 bacteriophage particles to specific locations on a patterned gold surface. Surface plasmon resonance demonstrated that free ACID peptides will assemble onto a surface derivatized with BASE. We then displayed the ACID peptide on the pIX coat protein of M13 and showed that these phage particles permit formation of the coiled-coil resulting in specific surface attachment. The ACID:immobilized BASE affinities appear to be similar for free peptide and phage-displayed ACID. Finally, we fabricated two gold electrodes, separated by a 200 nm gap, coated one of them with BASE and showed that this allows localization of the M13:ACID onto the functionalized electrode. |
format | Online Article Text |
id | pubmed-4785676 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
record_format | MEDLINE/PubMed |
spelling | pubmed-47856762016-03-10 Directed surface attachment of nanomaterials via coiled-coil-driven self-assembly White, Simon J Johnson, Steven Szymonik, Michal Wardingley, Richard A Pye, Douglas Davies, A Giles Wälti, Christoph Stockley, Peter G Nanotechnology Article Numerous nanoscale devices and materials have been fabricated in recent years using a variety of biological scaffolds. However, the interfacing of these devices and materials into existing circuits and ordered arrays has proved problematic. Here, we describe a simple solution to this problem using self-assembly of the peptide coiled-coil heterodimer ACID:BASE to immobilize M13 bacteriophage particles to specific locations on a patterned gold surface. Surface plasmon resonance demonstrated that free ACID peptides will assemble onto a surface derivatized with BASE. We then displayed the ACID peptide on the pIX coat protein of M13 and showed that these phage particles permit formation of the coiled-coil resulting in specific surface attachment. The ACID:immobilized BASE affinities appear to be similar for free peptide and phage-displayed ACID. Finally, we fabricated two gold electrodes, separated by a 200 nm gap, coated one of them with BASE and showed that this allows localization of the M13:ACID onto the functionalized electrode. 2012-11-16 2012-12-14 /pmc/articles/PMC4785676/ /pubmed/23154792 http://dx.doi.org/10.1088/0957-4484/23/49/495304 Text en Content from this work may be used under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 licence (http://creativecommons.org/licenses/by-nc-sa/3.0/) . Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI. |
spellingShingle | Article White, Simon J Johnson, Steven Szymonik, Michal Wardingley, Richard A Pye, Douglas Davies, A Giles Wälti, Christoph Stockley, Peter G Directed surface attachment of nanomaterials via coiled-coil-driven self-assembly |
title | Directed surface attachment of nanomaterials via coiled-coil-driven self-assembly |
title_full | Directed surface attachment of nanomaterials via coiled-coil-driven self-assembly |
title_fullStr | Directed surface attachment of nanomaterials via coiled-coil-driven self-assembly |
title_full_unstemmed | Directed surface attachment of nanomaterials via coiled-coil-driven self-assembly |
title_short | Directed surface attachment of nanomaterials via coiled-coil-driven self-assembly |
title_sort | directed surface attachment of nanomaterials via coiled-coil-driven self-assembly |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4785676/ https://www.ncbi.nlm.nih.gov/pubmed/23154792 http://dx.doi.org/10.1088/0957-4484/23/49/495304 |
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