Cargando…

Specific Detection of Two Divergent Simian Arteriviruses Using RNAscope In Situ Hybridization

Simian hemorrhagic fever (SHF) is an often lethal disease of Asian macaques. Simian hemorrhagic fever virus (SHFV) is one of at least three distinct simian arteriviruses that can cause SHF, but pathogenesis studies using modern methods have been scarce. Even seemingly straightforward studies, such a...

Descripción completa

Detalles Bibliográficos
Autores principales: Yú, Shuǐqìng, Caì, Yíngyún, Lyons, Cassandra, Johnson, Reed F., Postnikova, Elena, Mazur, Steven, Johnson, Joshua C., Radoshitzky, Sheli R., Bailey, Adam L., Lauck, Michael, Goldberg, Tony L., O’Connor, David H., Jahrling, Peter B., Friedrich, Thomas C., Kuhn, Jens H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4786270/
https://www.ncbi.nlm.nih.gov/pubmed/26963736
http://dx.doi.org/10.1371/journal.pone.0151313
_version_ 1782420528312614912
author Yú, Shuǐqìng
Caì, Yíngyún
Lyons, Cassandra
Johnson, Reed F.
Postnikova, Elena
Mazur, Steven
Johnson, Joshua C.
Radoshitzky, Sheli R.
Bailey, Adam L.
Lauck, Michael
Goldberg, Tony L.
O’Connor, David H.
Jahrling, Peter B.
Friedrich, Thomas C.
Kuhn, Jens H.
author_facet Yú, Shuǐqìng
Caì, Yíngyún
Lyons, Cassandra
Johnson, Reed F.
Postnikova, Elena
Mazur, Steven
Johnson, Joshua C.
Radoshitzky, Sheli R.
Bailey, Adam L.
Lauck, Michael
Goldberg, Tony L.
O’Connor, David H.
Jahrling, Peter B.
Friedrich, Thomas C.
Kuhn, Jens H.
author_sort Yú, Shuǐqìng
collection PubMed
description Simian hemorrhagic fever (SHF) is an often lethal disease of Asian macaques. Simian hemorrhagic fever virus (SHFV) is one of at least three distinct simian arteriviruses that can cause SHF, but pathogenesis studies using modern methods have been scarce. Even seemingly straightforward studies, such as examining viral tissue and cell tropism in vivo, have been difficult to conduct due to the absence of standardized SHFV-specific reagents. Here we report the establishment of an in situ hybridization assay for the detection of SHFV and distantly related Kibale red colobus virus 1 (KRCV-1) RNA in cell culture. In addition, we detected SHFV RNA in formalin-fixed, paraffin-embedded tissues from an infected rhesus monkey (Macaca mulatta). The assay is easily performed and can clearly distinguish between SHFV and KRCV-1. Thus, if further developed, this assay may be useful during future studies evaluating the mechanisms by which a simian arterivirus with a restricted cell tropism can cause a lethal nonhuman primate disease similar in clinical presentation to human viral hemorrhagic fevers.
format Online
Article
Text
id pubmed-4786270
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-47862702016-03-23 Specific Detection of Two Divergent Simian Arteriviruses Using RNAscope In Situ Hybridization Yú, Shuǐqìng Caì, Yíngyún Lyons, Cassandra Johnson, Reed F. Postnikova, Elena Mazur, Steven Johnson, Joshua C. Radoshitzky, Sheli R. Bailey, Adam L. Lauck, Michael Goldberg, Tony L. O’Connor, David H. Jahrling, Peter B. Friedrich, Thomas C. Kuhn, Jens H. PLoS One Research Article Simian hemorrhagic fever (SHF) is an often lethal disease of Asian macaques. Simian hemorrhagic fever virus (SHFV) is one of at least three distinct simian arteriviruses that can cause SHF, but pathogenesis studies using modern methods have been scarce. Even seemingly straightforward studies, such as examining viral tissue and cell tropism in vivo, have been difficult to conduct due to the absence of standardized SHFV-specific reagents. Here we report the establishment of an in situ hybridization assay for the detection of SHFV and distantly related Kibale red colobus virus 1 (KRCV-1) RNA in cell culture. In addition, we detected SHFV RNA in formalin-fixed, paraffin-embedded tissues from an infected rhesus monkey (Macaca mulatta). The assay is easily performed and can clearly distinguish between SHFV and KRCV-1. Thus, if further developed, this assay may be useful during future studies evaluating the mechanisms by which a simian arterivirus with a restricted cell tropism can cause a lethal nonhuman primate disease similar in clinical presentation to human viral hemorrhagic fevers. Public Library of Science 2016-03-10 /pmc/articles/PMC4786270/ /pubmed/26963736 http://dx.doi.org/10.1371/journal.pone.0151313 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Yú, Shuǐqìng
Caì, Yíngyún
Lyons, Cassandra
Johnson, Reed F.
Postnikova, Elena
Mazur, Steven
Johnson, Joshua C.
Radoshitzky, Sheli R.
Bailey, Adam L.
Lauck, Michael
Goldberg, Tony L.
O’Connor, David H.
Jahrling, Peter B.
Friedrich, Thomas C.
Kuhn, Jens H.
Specific Detection of Two Divergent Simian Arteriviruses Using RNAscope In Situ Hybridization
title Specific Detection of Two Divergent Simian Arteriviruses Using RNAscope In Situ Hybridization
title_full Specific Detection of Two Divergent Simian Arteriviruses Using RNAscope In Situ Hybridization
title_fullStr Specific Detection of Two Divergent Simian Arteriviruses Using RNAscope In Situ Hybridization
title_full_unstemmed Specific Detection of Two Divergent Simian Arteriviruses Using RNAscope In Situ Hybridization
title_short Specific Detection of Two Divergent Simian Arteriviruses Using RNAscope In Situ Hybridization
title_sort specific detection of two divergent simian arteriviruses using rnascope in situ hybridization
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4786270/
https://www.ncbi.nlm.nih.gov/pubmed/26963736
http://dx.doi.org/10.1371/journal.pone.0151313
work_keys_str_mv AT yushuiqing specificdetectionoftwodivergentsimianarterivirusesusingrnascopeinsituhybridization
AT caiyingyun specificdetectionoftwodivergentsimianarterivirusesusingrnascopeinsituhybridization
AT lyonscassandra specificdetectionoftwodivergentsimianarterivirusesusingrnascopeinsituhybridization
AT johnsonreedf specificdetectionoftwodivergentsimianarterivirusesusingrnascopeinsituhybridization
AT postnikovaelena specificdetectionoftwodivergentsimianarterivirusesusingrnascopeinsituhybridization
AT mazursteven specificdetectionoftwodivergentsimianarterivirusesusingrnascopeinsituhybridization
AT johnsonjoshuac specificdetectionoftwodivergentsimianarterivirusesusingrnascopeinsituhybridization
AT radoshitzkyshelir specificdetectionoftwodivergentsimianarterivirusesusingrnascopeinsituhybridization
AT baileyadaml specificdetectionoftwodivergentsimianarterivirusesusingrnascopeinsituhybridization
AT lauckmichael specificdetectionoftwodivergentsimianarterivirusesusingrnascopeinsituhybridization
AT goldbergtonyl specificdetectionoftwodivergentsimianarterivirusesusingrnascopeinsituhybridization
AT oconnordavidh specificdetectionoftwodivergentsimianarterivirusesusingrnascopeinsituhybridization
AT jahrlingpeterb specificdetectionoftwodivergentsimianarterivirusesusingrnascopeinsituhybridization
AT friedrichthomasc specificdetectionoftwodivergentsimianarterivirusesusingrnascopeinsituhybridization
AT kuhnjensh specificdetectionoftwodivergentsimianarterivirusesusingrnascopeinsituhybridization