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Specific Detection of Two Divergent Simian Arteriviruses Using RNAscope In Situ Hybridization
Simian hemorrhagic fever (SHF) is an often lethal disease of Asian macaques. Simian hemorrhagic fever virus (SHFV) is one of at least three distinct simian arteriviruses that can cause SHF, but pathogenesis studies using modern methods have been scarce. Even seemingly straightforward studies, such a...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4786270/ https://www.ncbi.nlm.nih.gov/pubmed/26963736 http://dx.doi.org/10.1371/journal.pone.0151313 |
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author | Yú, Shuǐqìng Caì, Yíngyún Lyons, Cassandra Johnson, Reed F. Postnikova, Elena Mazur, Steven Johnson, Joshua C. Radoshitzky, Sheli R. Bailey, Adam L. Lauck, Michael Goldberg, Tony L. O’Connor, David H. Jahrling, Peter B. Friedrich, Thomas C. Kuhn, Jens H. |
author_facet | Yú, Shuǐqìng Caì, Yíngyún Lyons, Cassandra Johnson, Reed F. Postnikova, Elena Mazur, Steven Johnson, Joshua C. Radoshitzky, Sheli R. Bailey, Adam L. Lauck, Michael Goldberg, Tony L. O’Connor, David H. Jahrling, Peter B. Friedrich, Thomas C. Kuhn, Jens H. |
author_sort | Yú, Shuǐqìng |
collection | PubMed |
description | Simian hemorrhagic fever (SHF) is an often lethal disease of Asian macaques. Simian hemorrhagic fever virus (SHFV) is one of at least three distinct simian arteriviruses that can cause SHF, but pathogenesis studies using modern methods have been scarce. Even seemingly straightforward studies, such as examining viral tissue and cell tropism in vivo, have been difficult to conduct due to the absence of standardized SHFV-specific reagents. Here we report the establishment of an in situ hybridization assay for the detection of SHFV and distantly related Kibale red colobus virus 1 (KRCV-1) RNA in cell culture. In addition, we detected SHFV RNA in formalin-fixed, paraffin-embedded tissues from an infected rhesus monkey (Macaca mulatta). The assay is easily performed and can clearly distinguish between SHFV and KRCV-1. Thus, if further developed, this assay may be useful during future studies evaluating the mechanisms by which a simian arterivirus with a restricted cell tropism can cause a lethal nonhuman primate disease similar in clinical presentation to human viral hemorrhagic fevers. |
format | Online Article Text |
id | pubmed-4786270 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-47862702016-03-23 Specific Detection of Two Divergent Simian Arteriviruses Using RNAscope In Situ Hybridization Yú, Shuǐqìng Caì, Yíngyún Lyons, Cassandra Johnson, Reed F. Postnikova, Elena Mazur, Steven Johnson, Joshua C. Radoshitzky, Sheli R. Bailey, Adam L. Lauck, Michael Goldberg, Tony L. O’Connor, David H. Jahrling, Peter B. Friedrich, Thomas C. Kuhn, Jens H. PLoS One Research Article Simian hemorrhagic fever (SHF) is an often lethal disease of Asian macaques. Simian hemorrhagic fever virus (SHFV) is one of at least three distinct simian arteriviruses that can cause SHF, but pathogenesis studies using modern methods have been scarce. Even seemingly straightforward studies, such as examining viral tissue and cell tropism in vivo, have been difficult to conduct due to the absence of standardized SHFV-specific reagents. Here we report the establishment of an in situ hybridization assay for the detection of SHFV and distantly related Kibale red colobus virus 1 (KRCV-1) RNA in cell culture. In addition, we detected SHFV RNA in formalin-fixed, paraffin-embedded tissues from an infected rhesus monkey (Macaca mulatta). The assay is easily performed and can clearly distinguish between SHFV and KRCV-1. Thus, if further developed, this assay may be useful during future studies evaluating the mechanisms by which a simian arterivirus with a restricted cell tropism can cause a lethal nonhuman primate disease similar in clinical presentation to human viral hemorrhagic fevers. Public Library of Science 2016-03-10 /pmc/articles/PMC4786270/ /pubmed/26963736 http://dx.doi.org/10.1371/journal.pone.0151313 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Yú, Shuǐqìng Caì, Yíngyún Lyons, Cassandra Johnson, Reed F. Postnikova, Elena Mazur, Steven Johnson, Joshua C. Radoshitzky, Sheli R. Bailey, Adam L. Lauck, Michael Goldberg, Tony L. O’Connor, David H. Jahrling, Peter B. Friedrich, Thomas C. Kuhn, Jens H. Specific Detection of Two Divergent Simian Arteriviruses Using RNAscope In Situ Hybridization |
title | Specific Detection of Two Divergent Simian Arteriviruses Using RNAscope In Situ Hybridization |
title_full | Specific Detection of Two Divergent Simian Arteriviruses Using RNAscope In Situ Hybridization |
title_fullStr | Specific Detection of Two Divergent Simian Arteriviruses Using RNAscope In Situ Hybridization |
title_full_unstemmed | Specific Detection of Two Divergent Simian Arteriviruses Using RNAscope In Situ Hybridization |
title_short | Specific Detection of Two Divergent Simian Arteriviruses Using RNAscope In Situ Hybridization |
title_sort | specific detection of two divergent simian arteriviruses using rnascope in situ hybridization |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4786270/ https://www.ncbi.nlm.nih.gov/pubmed/26963736 http://dx.doi.org/10.1371/journal.pone.0151313 |
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