In silico assessment of primers for eDNA studies using PrimerTree and application to characterize the biodiversity surrounding the Cuyahoga River

Analysis of environmental DNA (eDNA) enables the detection of species of interest from water and soil samples, typically using species-specific PCR. Here, we describe a method to characterize the biodiversity of a given environment by amplifying eDNA using primer pairs targeting a wide range of taxa...

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Detalles Bibliográficos
Autores principales: Cannon, M. V., Hester, J., Shalkhauser, A., Chan, E. R., Logue, K., Small, S. T., Serre, D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4786790/
https://www.ncbi.nlm.nih.gov/pubmed/26965911
http://dx.doi.org/10.1038/srep22908

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