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Spacer-free BODIPY fluorogens in antimicrobial peptides for direct imaging of fungal infection in human tissue
Fluorescent antimicrobial peptides are promising structures for in situ, real-time imaging of fungal infection. Here we report a fluorogenic probe to image Aspergillus fumigatus directly in human pulmonary tissue. We have developed a fluorogenic Trp-BODIPY amino acid with a spacer-free C-C linkage b...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4786873/ https://www.ncbi.nlm.nih.gov/pubmed/26956772 http://dx.doi.org/10.1038/ncomms10940 |
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author | Mendive-Tapia, Lorena Zhao, Can Akram, Ahsan R. Preciado, Sara Albericio, Fernando Lee, Martin Serrels, Alan Kielland, Nicola Read, Nick D Lavilla, Rodolfo Vendrell, Marc |
author_facet | Mendive-Tapia, Lorena Zhao, Can Akram, Ahsan R. Preciado, Sara Albericio, Fernando Lee, Martin Serrels, Alan Kielland, Nicola Read, Nick D Lavilla, Rodolfo Vendrell, Marc |
author_sort | Mendive-Tapia, Lorena |
collection | PubMed |
description | Fluorescent antimicrobial peptides are promising structures for in situ, real-time imaging of fungal infection. Here we report a fluorogenic probe to image Aspergillus fumigatus directly in human pulmonary tissue. We have developed a fluorogenic Trp-BODIPY amino acid with a spacer-free C-C linkage between Trp and a BODIPY fluorogen, which shows remarkable fluorescence enhancement in hydrophobic microenvironments. The incorporation of our fluorogenic amino acid in short antimicrobial peptides does not impair their selectivity for fungal cells, and enables rapid and direct fungal imaging without any washing steps. We have optimized the stability of our probes in human samples to perform multi-photon imaging of A. fumigatus in ex vivo human tissue. The incorporation of our unique BODIPY fluorogen in biologically relevant peptides will accelerate the development of novel imaging probes with high sensitivity and specificity. |
format | Online Article Text |
id | pubmed-4786873 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-47868732016-03-16 Spacer-free BODIPY fluorogens in antimicrobial peptides for direct imaging of fungal infection in human tissue Mendive-Tapia, Lorena Zhao, Can Akram, Ahsan R. Preciado, Sara Albericio, Fernando Lee, Martin Serrels, Alan Kielland, Nicola Read, Nick D Lavilla, Rodolfo Vendrell, Marc Nat Commun Article Fluorescent antimicrobial peptides are promising structures for in situ, real-time imaging of fungal infection. Here we report a fluorogenic probe to image Aspergillus fumigatus directly in human pulmonary tissue. We have developed a fluorogenic Trp-BODIPY amino acid with a spacer-free C-C linkage between Trp and a BODIPY fluorogen, which shows remarkable fluorescence enhancement in hydrophobic microenvironments. The incorporation of our fluorogenic amino acid in short antimicrobial peptides does not impair their selectivity for fungal cells, and enables rapid and direct fungal imaging without any washing steps. We have optimized the stability of our probes in human samples to perform multi-photon imaging of A. fumigatus in ex vivo human tissue. The incorporation of our unique BODIPY fluorogen in biologically relevant peptides will accelerate the development of novel imaging probes with high sensitivity and specificity. Nature Publishing Group 2016-03-09 /pmc/articles/PMC4786873/ /pubmed/26956772 http://dx.doi.org/10.1038/ncomms10940 Text en Copyright © 2016, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Mendive-Tapia, Lorena Zhao, Can Akram, Ahsan R. Preciado, Sara Albericio, Fernando Lee, Martin Serrels, Alan Kielland, Nicola Read, Nick D Lavilla, Rodolfo Vendrell, Marc Spacer-free BODIPY fluorogens in antimicrobial peptides for direct imaging of fungal infection in human tissue |
title | Spacer-free BODIPY fluorogens in antimicrobial peptides for direct imaging of fungal infection in human tissue |
title_full | Spacer-free BODIPY fluorogens in antimicrobial peptides for direct imaging of fungal infection in human tissue |
title_fullStr | Spacer-free BODIPY fluorogens in antimicrobial peptides for direct imaging of fungal infection in human tissue |
title_full_unstemmed | Spacer-free BODIPY fluorogens in antimicrobial peptides for direct imaging of fungal infection in human tissue |
title_short | Spacer-free BODIPY fluorogens in antimicrobial peptides for direct imaging of fungal infection in human tissue |
title_sort | spacer-free bodipy fluorogens in antimicrobial peptides for direct imaging of fungal infection in human tissue |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4786873/ https://www.ncbi.nlm.nih.gov/pubmed/26956772 http://dx.doi.org/10.1038/ncomms10940 |
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