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H(2)O(2) Improves Quality of Radix scutellariae Through Anti-oxidant Effect

INTRODUCTION: The correlation between the quality and geographical origin of herbal medicine was traced back to Tang Dynasty in China, more than 1200 years, and the effects of ecological environments on the secondary metabolites such as flavonoids have been confirmed. However, little is known about...

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Autores principales: Qi, Song, Wu-lin, Cao, Hua, Jiang, Ai-hua, Zhang, Xiang-cai, Meng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4787343/
https://www.ncbi.nlm.nih.gov/pubmed/27019566
http://dx.doi.org/10.4103/0973-1296.176063
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author Qi, Song
Wu-lin, Cao
Hua, Jiang
Ai-hua, Zhang
Xiang-cai, Meng
author_facet Qi, Song
Wu-lin, Cao
Hua, Jiang
Ai-hua, Zhang
Xiang-cai, Meng
author_sort Qi, Song
collection PubMed
description INTRODUCTION: The correlation between the quality and geographical origin of herbal medicine was traced back to Tang Dynasty in China, more than 1200 years, and the effects of ecological environments on the secondary metabolites such as flavonoids have been confirmed. However, little is known about how the adversity impacts on the quality. Reactive oxygen species (ROS) may be medium between the ecological environment and the secondary metabolism. MATERIALS AND METHODS: The fresh roots of Scutellaria baicalensis Georgi were treated with 0.002 μmol/L, 0.2 μmol/L, and 20 μmol/L H(2)O(2), respectively. A stress model was established to elucidate the change of secondary metabolism, anti-oxidant enzyme system, and enzymes relating to flavonoids. RESULTS: The activities of superoxide dismutase, catalase and peroxidase decreased. Too much H(2)O(2), firstly, boosted transformation of flavonoids glycoside into aglucon with the most remarkable activities through UDP-glucuronate baicalein 7-O-glucuronosyltransferase (UBGAT), and β-glucuronidase (GUS), then regulated the gene expression of phenylalanine ammonialyase, GUS, and UBGAT, and increased the contents of flavones, motivated the flavonoid glycoside converting into aglucon. With this action, the flavones displaced the anti-oxidant enzymes. The higher the dosage, the more baicalein and wogonin increased, the later they took action. CONCLUSION: The plant secondary metabolites to keep ROS constant are identical to the effective materials in clinic. They are closely linked. H(2)O(2) can improve flavones, especially the aglucon, and further increased the quality of herbal medicine, which possesses very important value in medical practice. SUMMARY: H(2)O(2) decreasing the activities of CAT and POD lead to accumulation of more H(2)O(2). Excess of H(2)O(2) up-regulated PAL, BUG, promote biosynthesis of flavones, and enhance the nonenzyme system. “↑” and “↓” represent activity or content “up” and “down” respectively.
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spelling pubmed-47873432016-03-25 H(2)O(2) Improves Quality of Radix scutellariae Through Anti-oxidant Effect Qi, Song Wu-lin, Cao Hua, Jiang Ai-hua, Zhang Xiang-cai, Meng Pharmacogn Mag Original Article INTRODUCTION: The correlation between the quality and geographical origin of herbal medicine was traced back to Tang Dynasty in China, more than 1200 years, and the effects of ecological environments on the secondary metabolites such as flavonoids have been confirmed. However, little is known about how the adversity impacts on the quality. Reactive oxygen species (ROS) may be medium between the ecological environment and the secondary metabolism. MATERIALS AND METHODS: The fresh roots of Scutellaria baicalensis Georgi were treated with 0.002 μmol/L, 0.2 μmol/L, and 20 μmol/L H(2)O(2), respectively. A stress model was established to elucidate the change of secondary metabolism, anti-oxidant enzyme system, and enzymes relating to flavonoids. RESULTS: The activities of superoxide dismutase, catalase and peroxidase decreased. Too much H(2)O(2), firstly, boosted transformation of flavonoids glycoside into aglucon with the most remarkable activities through UDP-glucuronate baicalein 7-O-glucuronosyltransferase (UBGAT), and β-glucuronidase (GUS), then regulated the gene expression of phenylalanine ammonialyase, GUS, and UBGAT, and increased the contents of flavones, motivated the flavonoid glycoside converting into aglucon. With this action, the flavones displaced the anti-oxidant enzymes. The higher the dosage, the more baicalein and wogonin increased, the later they took action. CONCLUSION: The plant secondary metabolites to keep ROS constant are identical to the effective materials in clinic. They are closely linked. H(2)O(2) can improve flavones, especially the aglucon, and further increased the quality of herbal medicine, which possesses very important value in medical practice. SUMMARY: H(2)O(2) decreasing the activities of CAT and POD lead to accumulation of more H(2)O(2). Excess of H(2)O(2) up-regulated PAL, BUG, promote biosynthesis of flavones, and enhance the nonenzyme system. “↑” and “↓” represent activity or content “up” and “down” respectively. Medknow Publications & Media Pvt Ltd 2016 /pmc/articles/PMC4787343/ /pubmed/27019566 http://dx.doi.org/10.4103/0973-1296.176063 Text en Copyright: © Pharmacognosy Magazine http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution NonCommercial ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Qi, Song
Wu-lin, Cao
Hua, Jiang
Ai-hua, Zhang
Xiang-cai, Meng
H(2)O(2) Improves Quality of Radix scutellariae Through Anti-oxidant Effect
title H(2)O(2) Improves Quality of Radix scutellariae Through Anti-oxidant Effect
title_full H(2)O(2) Improves Quality of Radix scutellariae Through Anti-oxidant Effect
title_fullStr H(2)O(2) Improves Quality of Radix scutellariae Through Anti-oxidant Effect
title_full_unstemmed H(2)O(2) Improves Quality of Radix scutellariae Through Anti-oxidant Effect
title_short H(2)O(2) Improves Quality of Radix scutellariae Through Anti-oxidant Effect
title_sort h(2)o(2) improves quality of radix scutellariae through anti-oxidant effect
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4787343/
https://www.ncbi.nlm.nih.gov/pubmed/27019566
http://dx.doi.org/10.4103/0973-1296.176063
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