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Enriching CRISPR-Cas9 targeted cells by co-targeting the HPRT gene

The CRISPR-Cas9 system uses guide RNAs to direct the Cas9 endonuclease to cleave target sequences. It can, in theory, target essentially any sequence in a genome, but the efficiency of the predicted guide RNAs varies dramatically. If no targeted cells are obtained, it is also difficult to know why t...

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Detalles Bibliográficos
Autores principales:	Liao, Shuren, Tammaro, Margaret, Yan, Hong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2015
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4787791/ https://www.ncbi.nlm.nih.gov/pubmed/26130722 http://dx.doi.org/10.1093/nar/gkv675

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