Cargando…
Soil-Derived Microbial Consortia Enriched with Different Plant Biomass Reveal Distinct Players Acting in Lignocellulose Degradation
Here, we investigated how different plant biomass, and—for one substrate—pH, drive the composition of degrader microbial consortia. We bred such consortia from forest soil, incubated along nine aerobic sequential - batch enrichments with wheat straw (WS1, pH 7.2; WS2, pH 9.0), switchgrass (SG, pH 7....
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4788684/ https://www.ncbi.nlm.nih.gov/pubmed/26487437 http://dx.doi.org/10.1007/s00248-015-0683-7 |
_version_ | 1782420752198270976 |
---|---|
author | de Lima Brossi, Maria Julia Jiménez, Diego Javier Cortes-Tolalpa, Larisa van Elsas, Jan Dirk |
author_facet | de Lima Brossi, Maria Julia Jiménez, Diego Javier Cortes-Tolalpa, Larisa van Elsas, Jan Dirk |
author_sort | de Lima Brossi, Maria Julia |
collection | PubMed |
description | Here, we investigated how different plant biomass, and—for one substrate—pH, drive the composition of degrader microbial consortia. We bred such consortia from forest soil, incubated along nine aerobic sequential - batch enrichments with wheat straw (WS1, pH 7.2; WS2, pH 9.0), switchgrass (SG, pH 7.2), and corn stover (CS, pH 7.2) as carbon sources. Lignocellulosic compounds (lignin, cellulose and xylan) were best degraded in treatment SG, followed by CS, WS1 and WS2. In terms of composition, the consortia became relatively stable after transfers 4 to 6, as evidenced by PCR-DGGE profiles obtained from each consortium DNA. The final consortia differed by ~40 % (bacteria) and ~60 % (fungi) across treatments. A ‘core’ community represented by 5/16 (bacteria) and 3/14 (fungi) bands was discerned, next to a variable part. The composition of the final microbial consortia was strongly driven by the substrate, as taxonomically-diverse consortia appeared in the different substrate treatments, but not in the (WS) different pH one. Biodegradative strains affiliated to Sphingobacterium kitahiroshimense, Raoultella terrigena, Pseudomonas putida, Stenotrophomonas rhizophila (bacteria), Coniochaeta ligniaria and Acremonium sp. (fungi) were recovered in at least three treatments, whereas strains affiliated to Delftia tsuruhatensis, Paenibacillus xylanexedens, Sanguibacter inulus and Comamonas jiangduensis were treatment-specific. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00248-015-0683-7) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4788684 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-47886842016-04-09 Soil-Derived Microbial Consortia Enriched with Different Plant Biomass Reveal Distinct Players Acting in Lignocellulose Degradation de Lima Brossi, Maria Julia Jiménez, Diego Javier Cortes-Tolalpa, Larisa van Elsas, Jan Dirk Microb Ecol Environmental Microbiology Here, we investigated how different plant biomass, and—for one substrate—pH, drive the composition of degrader microbial consortia. We bred such consortia from forest soil, incubated along nine aerobic sequential - batch enrichments with wheat straw (WS1, pH 7.2; WS2, pH 9.0), switchgrass (SG, pH 7.2), and corn stover (CS, pH 7.2) as carbon sources. Lignocellulosic compounds (lignin, cellulose and xylan) were best degraded in treatment SG, followed by CS, WS1 and WS2. In terms of composition, the consortia became relatively stable after transfers 4 to 6, as evidenced by PCR-DGGE profiles obtained from each consortium DNA. The final consortia differed by ~40 % (bacteria) and ~60 % (fungi) across treatments. A ‘core’ community represented by 5/16 (bacteria) and 3/14 (fungi) bands was discerned, next to a variable part. The composition of the final microbial consortia was strongly driven by the substrate, as taxonomically-diverse consortia appeared in the different substrate treatments, but not in the (WS) different pH one. Biodegradative strains affiliated to Sphingobacterium kitahiroshimense, Raoultella terrigena, Pseudomonas putida, Stenotrophomonas rhizophila (bacteria), Coniochaeta ligniaria and Acremonium sp. (fungi) were recovered in at least three treatments, whereas strains affiliated to Delftia tsuruhatensis, Paenibacillus xylanexedens, Sanguibacter inulus and Comamonas jiangduensis were treatment-specific. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00248-015-0683-7) contains supplementary material, which is available to authorized users. Springer US 2015-10-20 2016 /pmc/articles/PMC4788684/ /pubmed/26487437 http://dx.doi.org/10.1007/s00248-015-0683-7 Text en © The Author(s) 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Environmental Microbiology de Lima Brossi, Maria Julia Jiménez, Diego Javier Cortes-Tolalpa, Larisa van Elsas, Jan Dirk Soil-Derived Microbial Consortia Enriched with Different Plant Biomass Reveal Distinct Players Acting in Lignocellulose Degradation |
title | Soil-Derived Microbial Consortia Enriched with Different Plant Biomass Reveal Distinct Players Acting in Lignocellulose Degradation |
title_full | Soil-Derived Microbial Consortia Enriched with Different Plant Biomass Reveal Distinct Players Acting in Lignocellulose Degradation |
title_fullStr | Soil-Derived Microbial Consortia Enriched with Different Plant Biomass Reveal Distinct Players Acting in Lignocellulose Degradation |
title_full_unstemmed | Soil-Derived Microbial Consortia Enriched with Different Plant Biomass Reveal Distinct Players Acting in Lignocellulose Degradation |
title_short | Soil-Derived Microbial Consortia Enriched with Different Plant Biomass Reveal Distinct Players Acting in Lignocellulose Degradation |
title_sort | soil-derived microbial consortia enriched with different plant biomass reveal distinct players acting in lignocellulose degradation |
topic | Environmental Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4788684/ https://www.ncbi.nlm.nih.gov/pubmed/26487437 http://dx.doi.org/10.1007/s00248-015-0683-7 |
work_keys_str_mv | AT delimabrossimariajulia soilderivedmicrobialconsortiaenrichedwithdifferentplantbiomassrevealdistinctplayersactinginlignocellulosedegradation AT jimenezdiegojavier soilderivedmicrobialconsortiaenrichedwithdifferentplantbiomassrevealdistinctplayersactinginlignocellulosedegradation AT cortestolalpalarisa soilderivedmicrobialconsortiaenrichedwithdifferentplantbiomassrevealdistinctplayersactinginlignocellulosedegradation AT vanelsasjandirk soilderivedmicrobialconsortiaenrichedwithdifferentplantbiomassrevealdistinctplayersactinginlignocellulosedegradation |