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Shenhua Tablet inhibits mesangial cell proliferation in rats with chronic anti-Thy-1 nephritis

BACKGROUND: In China, mesangial proliferative glomerulonephritis (MsPGN) is one of the most common kidney diseases. In this study, we treated a rat model of chronic anti-Thy-1 MsPGN with Shenhua Tablet and evaluated whether the tablet was able to protect the kidney function. Thirty-six Wistar rats w...

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Autores principales: Geng, Wenjia, Wei, Ribao, Liu, Shuwen, Tang, Li, Zhu, Hanyu, Chen, Pu, Wu, Jie, Zhang, Xueguang, Zhu, Fei, Yin, Zhong, Chen, Xiangmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4788853/
https://www.ncbi.nlm.nih.gov/pubmed/26969153
http://dx.doi.org/10.1186/s40659-016-0078-3
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author Geng, Wenjia
Wei, Ribao
Liu, Shuwen
Tang, Li
Zhu, Hanyu
Chen, Pu
Wu, Jie
Zhang, Xueguang
Zhu, Fei
Yin, Zhong
Chen, Xiangmei
author_facet Geng, Wenjia
Wei, Ribao
Liu, Shuwen
Tang, Li
Zhu, Hanyu
Chen, Pu
Wu, Jie
Zhang, Xueguang
Zhu, Fei
Yin, Zhong
Chen, Xiangmei
author_sort Geng, Wenjia
collection PubMed
description BACKGROUND: In China, mesangial proliferative glomerulonephritis (MsPGN) is one of the most common kidney diseases. In this study, we treated a rat model of chronic anti-Thy-1 MsPGN with Shenhua Tablet and evaluated whether the tablet was able to protect the kidney function. Thirty-six Wistar rats were randomly divided into six groups: (1) Sham surgery (Sham); (2) anti-Thy-1 nephritis model (Thy-1); (3) anti-Thy-1 nephritis model + irbesartan-treated (Irb); (4) anti-Thy-1 nephritis model + low-dose of Shenhua Tablet (SHL); (5) anti-Thy-1 nephritis model + medium-dose of Shenhua Tablet (SHM); (6) anti-Thy-1 nephritis model + high-dose of Shenhua Tablet (SHH). RESULTS: Thirteen weeks after drug treatment, urinary proteins were quantified and renal pathological changes were thoroughly examined at the time point of 24 h. Meanwhile, the expression levels of p-Erk1/2, cyclin D1 and p21 at the renal cortex were also tested. The levels of urinary proteins and total cholesterol in the blood were significantly reduced in rats treated with any drug tested in this study. The level of triglyceride was significantly reduced in all three Shenhua Tablet-treated groups. Renal pathomorphological scores were significantly improved in groups of Irb, SHM and SHH. Mesangial cell proliferation was significantly inhibited in any drug-treated group. p-Erk1/2 and cyclin D1 were downregulated whereas p21 was upregulated in the renal cortex. CONCLUSIONS: Our study indicated that Shenhua Tablet is able to inhibit the abnormal proliferation of mesangial cells and to prevent kidney damage, which is likely associated with downregulation of p-Erk1/2 and reduced activity of its downstream target-cyclin D1.
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spelling pubmed-47888532016-03-13 Shenhua Tablet inhibits mesangial cell proliferation in rats with chronic anti-Thy-1 nephritis Geng, Wenjia Wei, Ribao Liu, Shuwen Tang, Li Zhu, Hanyu Chen, Pu Wu, Jie Zhang, Xueguang Zhu, Fei Yin, Zhong Chen, Xiangmei Biol Res Research Article BACKGROUND: In China, mesangial proliferative glomerulonephritis (MsPGN) is one of the most common kidney diseases. In this study, we treated a rat model of chronic anti-Thy-1 MsPGN with Shenhua Tablet and evaluated whether the tablet was able to protect the kidney function. Thirty-six Wistar rats were randomly divided into six groups: (1) Sham surgery (Sham); (2) anti-Thy-1 nephritis model (Thy-1); (3) anti-Thy-1 nephritis model + irbesartan-treated (Irb); (4) anti-Thy-1 nephritis model + low-dose of Shenhua Tablet (SHL); (5) anti-Thy-1 nephritis model + medium-dose of Shenhua Tablet (SHM); (6) anti-Thy-1 nephritis model + high-dose of Shenhua Tablet (SHH). RESULTS: Thirteen weeks after drug treatment, urinary proteins were quantified and renal pathological changes were thoroughly examined at the time point of 24 h. Meanwhile, the expression levels of p-Erk1/2, cyclin D1 and p21 at the renal cortex were also tested. The levels of urinary proteins and total cholesterol in the blood were significantly reduced in rats treated with any drug tested in this study. The level of triglyceride was significantly reduced in all three Shenhua Tablet-treated groups. Renal pathomorphological scores were significantly improved in groups of Irb, SHM and SHH. Mesangial cell proliferation was significantly inhibited in any drug-treated group. p-Erk1/2 and cyclin D1 were downregulated whereas p21 was upregulated in the renal cortex. CONCLUSIONS: Our study indicated that Shenhua Tablet is able to inhibit the abnormal proliferation of mesangial cells and to prevent kidney damage, which is likely associated with downregulation of p-Erk1/2 and reduced activity of its downstream target-cyclin D1. BioMed Central 2016-03-11 /pmc/articles/PMC4788853/ /pubmed/26969153 http://dx.doi.org/10.1186/s40659-016-0078-3 Text en © Geng et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Geng, Wenjia
Wei, Ribao
Liu, Shuwen
Tang, Li
Zhu, Hanyu
Chen, Pu
Wu, Jie
Zhang, Xueguang
Zhu, Fei
Yin, Zhong
Chen, Xiangmei
Shenhua Tablet inhibits mesangial cell proliferation in rats with chronic anti-Thy-1 nephritis
title Shenhua Tablet inhibits mesangial cell proliferation in rats with chronic anti-Thy-1 nephritis
title_full Shenhua Tablet inhibits mesangial cell proliferation in rats with chronic anti-Thy-1 nephritis
title_fullStr Shenhua Tablet inhibits mesangial cell proliferation in rats with chronic anti-Thy-1 nephritis
title_full_unstemmed Shenhua Tablet inhibits mesangial cell proliferation in rats with chronic anti-Thy-1 nephritis
title_short Shenhua Tablet inhibits mesangial cell proliferation in rats with chronic anti-Thy-1 nephritis
title_sort shenhua tablet inhibits mesangial cell proliferation in rats with chronic anti-thy-1 nephritis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4788853/
https://www.ncbi.nlm.nih.gov/pubmed/26969153
http://dx.doi.org/10.1186/s40659-016-0078-3
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