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Automation of antimicrobial activity screening
Manual and automated methods were compared for routine screening of compounds for antimicrobial activity. Automation generally accelerated assays and required less user intervention while producing comparable results. Automated protocols were validated for planktonic, biofilm, and agar cultures of t...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4788993/ https://www.ncbi.nlm.nih.gov/pubmed/26970766 http://dx.doi.org/10.1186/s13568-016-0191-2 |
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author | Forry, Samuel P. Madonna, Megan C. López-Pérez, Daneli Lin, Nancy J. Pasco, Madeleine D. |
author_facet | Forry, Samuel P. Madonna, Megan C. López-Pérez, Daneli Lin, Nancy J. Pasco, Madeleine D. |
author_sort | Forry, Samuel P. |
collection | PubMed |
description | Manual and automated methods were compared for routine screening of compounds for antimicrobial activity. Automation generally accelerated assays and required less user intervention while producing comparable results. Automated protocols were validated for planktonic, biofilm, and agar cultures of the oral microbe Streptococcus mutans that is commonly associated with tooth decay. Toxicity assays for the known antimicrobial compound cetylpyridinium chloride (CPC) were validated against planktonic, biofilm forming, and 24 h biofilm culture conditions, and several commonly reported toxicity/antimicrobial activity measures were evaluated: the 50 % inhibitory concentration (IC50), the minimum inhibitory concentration (MIC), and the minimum bactericidal concentration (MBC). Using automated methods, three halide salts of cetylpyridinium (CPC, CPB, CPI) were rapidly screened with no detectable effect of the counter ion on antimicrobial activity. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13568-016-0191-2) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4788993 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-47889932016-04-09 Automation of antimicrobial activity screening Forry, Samuel P. Madonna, Megan C. López-Pérez, Daneli Lin, Nancy J. Pasco, Madeleine D. AMB Express Original Article Manual and automated methods were compared for routine screening of compounds for antimicrobial activity. Automation generally accelerated assays and required less user intervention while producing comparable results. Automated protocols were validated for planktonic, biofilm, and agar cultures of the oral microbe Streptococcus mutans that is commonly associated with tooth decay. Toxicity assays for the known antimicrobial compound cetylpyridinium chloride (CPC) were validated against planktonic, biofilm forming, and 24 h biofilm culture conditions, and several commonly reported toxicity/antimicrobial activity measures were evaluated: the 50 % inhibitory concentration (IC50), the minimum inhibitory concentration (MIC), and the minimum bactericidal concentration (MBC). Using automated methods, three halide salts of cetylpyridinium (CPC, CPB, CPI) were rapidly screened with no detectable effect of the counter ion on antimicrobial activity. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13568-016-0191-2) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2016-03-12 /pmc/articles/PMC4788993/ /pubmed/26970766 http://dx.doi.org/10.1186/s13568-016-0191-2 Text en © Forry et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Forry, Samuel P. Madonna, Megan C. López-Pérez, Daneli Lin, Nancy J. Pasco, Madeleine D. Automation of antimicrobial activity screening |
title | Automation of antimicrobial activity screening |
title_full | Automation of antimicrobial activity screening |
title_fullStr | Automation of antimicrobial activity screening |
title_full_unstemmed | Automation of antimicrobial activity screening |
title_short | Automation of antimicrobial activity screening |
title_sort | automation of antimicrobial activity screening |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4788993/ https://www.ncbi.nlm.nih.gov/pubmed/26970766 http://dx.doi.org/10.1186/s13568-016-0191-2 |
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