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Components and Anti-HepG2 Activity Comparison of Lycopodium Alkaloids from Four Geographic Origins

Lycopodium japonicum Thunb. has attracted great interests due to its rich alkaloids with significant anticancer activity. However, significant chemical differences often exist in a plant species from different geographic origins and affect its quality and bioactivities. Thus, it is urgent to reveal...

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Autores principales: Tian, Yong-Qiang, Hu, Guang-Wan, Guo, Ming-Quan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4789059/
https://www.ncbi.nlm.nih.gov/pubmed/27022402
http://dx.doi.org/10.1155/2016/4631843
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author Tian, Yong-Qiang
Hu, Guang-Wan
Guo, Ming-Quan
author_facet Tian, Yong-Qiang
Hu, Guang-Wan
Guo, Ming-Quan
author_sort Tian, Yong-Qiang
collection PubMed
description Lycopodium japonicum Thunb. has attracted great interests due to its rich alkaloids with significant anticancer activity. However, significant chemical differences often exist in a plant species from different geographic origins and affect its quality and bioactivities. Thus, it is urgent to reveal their chemical and biological distinctions at the molecular level. In this context, a comparative chemical analysis of LAs using HPLC-UV-ESI-MS/MS was firstly conducted and resulted in the detection of 46 LAs, 28 of which were identified, and a series of unique LAs markers, such as peaks 2, 9, 10, and 11, were further found to be characteristic LAs and selected as markers from four different origins for their quality control. In parallel, the comparative bioactivity assay revealed that the total LAs from Hubei province exhibited much higher inhibitory rate at 65.95% against HepG2 cells than those at 26.72%, 20.26%, and 33.62% for Kenya, Guangxi province, and Zhejiang province in China, respectively. To this end, significant chemical fingerprinting differences and discrepancies in bioactivity of LAs were explored firstly, which could provide valuable information for quality control and further activity studies on LAs from different sources and promote their better pharmaceutical applications in the future as well.
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spelling pubmed-47890592016-03-28 Components and Anti-HepG2 Activity Comparison of Lycopodium Alkaloids from Four Geographic Origins Tian, Yong-Qiang Hu, Guang-Wan Guo, Ming-Quan Evid Based Complement Alternat Med Research Article Lycopodium japonicum Thunb. has attracted great interests due to its rich alkaloids with significant anticancer activity. However, significant chemical differences often exist in a plant species from different geographic origins and affect its quality and bioactivities. Thus, it is urgent to reveal their chemical and biological distinctions at the molecular level. In this context, a comparative chemical analysis of LAs using HPLC-UV-ESI-MS/MS was firstly conducted and resulted in the detection of 46 LAs, 28 of which were identified, and a series of unique LAs markers, such as peaks 2, 9, 10, and 11, were further found to be characteristic LAs and selected as markers from four different origins for their quality control. In parallel, the comparative bioactivity assay revealed that the total LAs from Hubei province exhibited much higher inhibitory rate at 65.95% against HepG2 cells than those at 26.72%, 20.26%, and 33.62% for Kenya, Guangxi province, and Zhejiang province in China, respectively. To this end, significant chemical fingerprinting differences and discrepancies in bioactivity of LAs were explored firstly, which could provide valuable information for quality control and further activity studies on LAs from different sources and promote their better pharmaceutical applications in the future as well. Hindawi Publishing Corporation 2016 2016-02-28 /pmc/articles/PMC4789059/ /pubmed/27022402 http://dx.doi.org/10.1155/2016/4631843 Text en Copyright © 2016 Yong-Qiang Tian et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tian, Yong-Qiang
Hu, Guang-Wan
Guo, Ming-Quan
Components and Anti-HepG2 Activity Comparison of Lycopodium Alkaloids from Four Geographic Origins
title Components and Anti-HepG2 Activity Comparison of Lycopodium Alkaloids from Four Geographic Origins
title_full Components and Anti-HepG2 Activity Comparison of Lycopodium Alkaloids from Four Geographic Origins
title_fullStr Components and Anti-HepG2 Activity Comparison of Lycopodium Alkaloids from Four Geographic Origins
title_full_unstemmed Components and Anti-HepG2 Activity Comparison of Lycopodium Alkaloids from Four Geographic Origins
title_short Components and Anti-HepG2 Activity Comparison of Lycopodium Alkaloids from Four Geographic Origins
title_sort components and anti-hepg2 activity comparison of lycopodium alkaloids from four geographic origins
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4789059/
https://www.ncbi.nlm.nih.gov/pubmed/27022402
http://dx.doi.org/10.1155/2016/4631843
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