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Efficient and simple generation of multiple unmarked gene deletions in Mycobacterium smegmatis
Research on mycobacterial genetics relies heavily on techniques for directed gene mutation, but genetic studies are often hampered by the difficulty of generating gene deletions in mycobacteria. We developed an efficient and improved deletion system, described here in detail, which can be used to co...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4789726/ https://www.ncbi.nlm.nih.gov/pubmed/26972108 http://dx.doi.org/10.1038/srep22922 |