Participation of S. Typhimurium cysJIH Operon in the H(2)S-mediated Ciprofloxacin Resistance in Presence of Sulfate as Sulfur Source

H(2)S production has been proposed as a mechanism to explain bacterial resistance to antibiotics. In this work, we present evidence for the role of the cysJIH operon in resistance to ciprofloxacin mediated by H(2)S production with different sulfate as the only sulfur source. We found that the produc...

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Detalles Bibliográficos
Autores principales: Álvarez, Ricardo, Frávega, Jorge, Rodas, Paula I., Fuentes, Juan A., Paredes-Sabja, Daniel, Calderón, Iván L., Gil, Fernando
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4790288/
http://dx.doi.org/10.3390/antibiotics4030321
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author Álvarez, Ricardo
Frávega, Jorge
Rodas, Paula I.
Fuentes, Juan A.
Paredes-Sabja, Daniel
Calderón, Iván L.
Gil, Fernando
author_facet Álvarez, Ricardo
Frávega, Jorge
Rodas, Paula I.
Fuentes, Juan A.
Paredes-Sabja, Daniel
Calderón, Iván L.
Gil, Fernando
author_sort Álvarez, Ricardo
collection PubMed
description H(2)S production has been proposed as a mechanism to explain bacterial resistance to antibiotics. In this work, we present evidence for the role of the cysJIH operon in resistance to ciprofloxacin mediated by H(2)S production with different sulfate as the only sulfur source. We found that the products of the cysJIH operon are involved in ciprofloxacin resistance by increasing both, the levels of H(2)S and reduced thiols apparently counteracting antimicrobial-induced reactive oxygen species (ROS). This protective effect was observed only when bacteria were cultured in the presence of sulfate, but not with cysteine, as the sole sulfur source.
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spelling pubmed-47902882016-03-24 Participation of S. Typhimurium cysJIH Operon in the H(2)S-mediated Ciprofloxacin Resistance in Presence of Sulfate as Sulfur Source Álvarez, Ricardo Frávega, Jorge Rodas, Paula I. Fuentes, Juan A. Paredes-Sabja, Daniel Calderón, Iván L. Gil, Fernando Antibiotics (Basel) Communication H(2)S production has been proposed as a mechanism to explain bacterial resistance to antibiotics. In this work, we present evidence for the role of the cysJIH operon in resistance to ciprofloxacin mediated by H(2)S production with different sulfate as the only sulfur source. We found that the products of the cysJIH operon are involved in ciprofloxacin resistance by increasing both, the levels of H(2)S and reduced thiols apparently counteracting antimicrobial-induced reactive oxygen species (ROS). This protective effect was observed only when bacteria were cultured in the presence of sulfate, but not with cysteine, as the sole sulfur source. MDPI 2015-07-31 /pmc/articles/PMC4790288/ http://dx.doi.org/10.3390/antibiotics4030321 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Álvarez, Ricardo
Frávega, Jorge
Rodas, Paula I.
Fuentes, Juan A.
Paredes-Sabja, Daniel
Calderón, Iván L.
Gil, Fernando
Participation of S. Typhimurium cysJIH Operon in the H(2)S-mediated Ciprofloxacin Resistance in Presence of Sulfate as Sulfur Source
title Participation of S. Typhimurium cysJIH Operon in the H(2)S-mediated Ciprofloxacin Resistance in Presence of Sulfate as Sulfur Source
title_full Participation of S. Typhimurium cysJIH Operon in the H(2)S-mediated Ciprofloxacin Resistance in Presence of Sulfate as Sulfur Source
title_fullStr Participation of S. Typhimurium cysJIH Operon in the H(2)S-mediated Ciprofloxacin Resistance in Presence of Sulfate as Sulfur Source
title_full_unstemmed Participation of S. Typhimurium cysJIH Operon in the H(2)S-mediated Ciprofloxacin Resistance in Presence of Sulfate as Sulfur Source
title_short Participation of S. Typhimurium cysJIH Operon in the H(2)S-mediated Ciprofloxacin Resistance in Presence of Sulfate as Sulfur Source
title_sort participation of s. typhimurium cysjih operon in the h(2)s-mediated ciprofloxacin resistance in presence of sulfate as sulfur source
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4790288/
http://dx.doi.org/10.3390/antibiotics4030321
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