Cargando…

Miniaturized Antimicrobial Susceptibility Test by Combining Concentration Gradient Generation and Rapid Cell Culturing

Effective treatment of bacterial infection relies on timely diagnosis and proper prescription of antibiotic drugs. The antimicrobial susceptibility test (AST) is one of the most crucial experimental procedures, providing the baseline information for choosing effective antibiotic agents and their dos...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Samuel C., Cestellos-Blanco, Stefano, Inoue, Keisuke, Zare, Richard N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4790307/
https://www.ncbi.nlm.nih.gov/pubmed/27025635
http://dx.doi.org/10.3390/antibiotics4040455
_version_ 1782420970633428992
author Kim, Samuel C.
Cestellos-Blanco, Stefano
Inoue, Keisuke
Zare, Richard N.
author_facet Kim, Samuel C.
Cestellos-Blanco, Stefano
Inoue, Keisuke
Zare, Richard N.
author_sort Kim, Samuel C.
collection PubMed
description Effective treatment of bacterial infection relies on timely diagnosis and proper prescription of antibiotic drugs. The antimicrobial susceptibility test (AST) is one of the most crucial experimental procedures, providing the baseline information for choosing effective antibiotic agents and their dosages. Conventional methods, however, require long incubation times or significant instrumentation costs to obtain test results. We propose a lab-on-a-chip approach to perform AST in a simple, economic, and rapid manner. Our assay platform miniaturizes the standard broth microdilution method on a microfluidic device (20 × 20 mm) that generates an antibiotic concentration gradient and delivers antibiotic-containing culture media to eight 30-nL chambers for cell culture. When tested with 20 μL samples of a model bacterial strain (E. coli ATCC 25922) treated with ampicillin or streptomycin, our method allows for the determination of minimum inhibitory concentrations consistent with the microdilution test in three hours, which is almost a factor of ten more rapid than the standard method.
format Online
Article
Text
id pubmed-4790307
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-47903072016-03-24 Miniaturized Antimicrobial Susceptibility Test by Combining Concentration Gradient Generation and Rapid Cell Culturing Kim, Samuel C. Cestellos-Blanco, Stefano Inoue, Keisuke Zare, Richard N. Antibiotics (Basel) Article Effective treatment of bacterial infection relies on timely diagnosis and proper prescription of antibiotic drugs. The antimicrobial susceptibility test (AST) is one of the most crucial experimental procedures, providing the baseline information for choosing effective antibiotic agents and their dosages. Conventional methods, however, require long incubation times or significant instrumentation costs to obtain test results. We propose a lab-on-a-chip approach to perform AST in a simple, economic, and rapid manner. Our assay platform miniaturizes the standard broth microdilution method on a microfluidic device (20 × 20 mm) that generates an antibiotic concentration gradient and delivers antibiotic-containing culture media to eight 30-nL chambers for cell culture. When tested with 20 μL samples of a model bacterial strain (E. coli ATCC 25922) treated with ampicillin or streptomycin, our method allows for the determination of minimum inhibitory concentrations consistent with the microdilution test in three hours, which is almost a factor of ten more rapid than the standard method. MDPI 2015-10-29 /pmc/articles/PMC4790307/ /pubmed/27025635 http://dx.doi.org/10.3390/antibiotics4040455 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Samuel C.
Cestellos-Blanco, Stefano
Inoue, Keisuke
Zare, Richard N.
Miniaturized Antimicrobial Susceptibility Test by Combining Concentration Gradient Generation and Rapid Cell Culturing
title Miniaturized Antimicrobial Susceptibility Test by Combining Concentration Gradient Generation and Rapid Cell Culturing
title_full Miniaturized Antimicrobial Susceptibility Test by Combining Concentration Gradient Generation and Rapid Cell Culturing
title_fullStr Miniaturized Antimicrobial Susceptibility Test by Combining Concentration Gradient Generation and Rapid Cell Culturing
title_full_unstemmed Miniaturized Antimicrobial Susceptibility Test by Combining Concentration Gradient Generation and Rapid Cell Culturing
title_short Miniaturized Antimicrobial Susceptibility Test by Combining Concentration Gradient Generation and Rapid Cell Culturing
title_sort miniaturized antimicrobial susceptibility test by combining concentration gradient generation and rapid cell culturing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4790307/
https://www.ncbi.nlm.nih.gov/pubmed/27025635
http://dx.doi.org/10.3390/antibiotics4040455
work_keys_str_mv AT kimsamuelc miniaturizedantimicrobialsusceptibilitytestbycombiningconcentrationgradientgenerationandrapidcellculturing
AT cestellosblancostefano miniaturizedantimicrobialsusceptibilitytestbycombiningconcentrationgradientgenerationandrapidcellculturing
AT inouekeisuke miniaturizedantimicrobialsusceptibilitytestbycombiningconcentrationgradientgenerationandrapidcellculturing
AT zarerichardn miniaturizedantimicrobialsusceptibilitytestbycombiningconcentrationgradientgenerationandrapidcellculturing