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Metabolism-related enzyme alterations identified by proteomic analysis in human renal cell carcinoma

The renal cell carcinoma (RCC) is one of the most common types of kidney neoplasia in Western countries; it is relatively resistant to conventional chemotherapy and radiotherapy. Metabolic disorders have a profound effect on the degree of malignancy and treatment resistance of the tumor. However, th...

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Autores principales: Lu, Zejun, Yao, Yuqin, Song, Qi, Yang, Jinliang, Zhao, Xiangfei, Yang, Ping, Kang, Jingbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4790526/
https://www.ncbi.nlm.nih.gov/pubmed/27022288
http://dx.doi.org/10.2147/OTT.S91953
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author Lu, Zejun
Yao, Yuqin
Song, Qi
Yang, Jinliang
Zhao, Xiangfei
Yang, Ping
Kang, Jingbo
author_facet Lu, Zejun
Yao, Yuqin
Song, Qi
Yang, Jinliang
Zhao, Xiangfei
Yang, Ping
Kang, Jingbo
author_sort Lu, Zejun
collection PubMed
description The renal cell carcinoma (RCC) is one of the most common types of kidney neoplasia in Western countries; it is relatively resistant to conventional chemotherapy and radiotherapy. Metabolic disorders have a profound effect on the degree of malignancy and treatment resistance of the tumor. However, the molecular characteristics related to impaired metabolism leading to the initiation of RCC are still not very clear. In this study, two-dimensional electrophoresis (2-DE) and mass spectra (MS) technologies were utilized to identify the proteins involved in energy metabolism of RCC. A total of 73 proteins that were differentially expressed in conventional RCC, in comparison with the corresponding normal kidney tissues, were identified. Bioinformatics analysis has shown that these proteins are involved in glycolysis, urea cycle, and the metabolic pathways of pyruvate, propanoate, and arginine/proline. In addition, some were also involved in the signaling network of p53 and FAS. These results provide some clues for new therapeutic targets and treatment strategies of RCC.
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spelling pubmed-47905262016-03-28 Metabolism-related enzyme alterations identified by proteomic analysis in human renal cell carcinoma Lu, Zejun Yao, Yuqin Song, Qi Yang, Jinliang Zhao, Xiangfei Yang, Ping Kang, Jingbo Onco Targets Ther Original Research The renal cell carcinoma (RCC) is one of the most common types of kidney neoplasia in Western countries; it is relatively resistant to conventional chemotherapy and radiotherapy. Metabolic disorders have a profound effect on the degree of malignancy and treatment resistance of the tumor. However, the molecular characteristics related to impaired metabolism leading to the initiation of RCC are still not very clear. In this study, two-dimensional electrophoresis (2-DE) and mass spectra (MS) technologies were utilized to identify the proteins involved in energy metabolism of RCC. A total of 73 proteins that were differentially expressed in conventional RCC, in comparison with the corresponding normal kidney tissues, were identified. Bioinformatics analysis has shown that these proteins are involved in glycolysis, urea cycle, and the metabolic pathways of pyruvate, propanoate, and arginine/proline. In addition, some were also involved in the signaling network of p53 and FAS. These results provide some clues for new therapeutic targets and treatment strategies of RCC. Dove Medical Press 2016-03-09 /pmc/articles/PMC4790526/ /pubmed/27022288 http://dx.doi.org/10.2147/OTT.S91953 Text en © 2016 Lu et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Lu, Zejun
Yao, Yuqin
Song, Qi
Yang, Jinliang
Zhao, Xiangfei
Yang, Ping
Kang, Jingbo
Metabolism-related enzyme alterations identified by proteomic analysis in human renal cell carcinoma
title Metabolism-related enzyme alterations identified by proteomic analysis in human renal cell carcinoma
title_full Metabolism-related enzyme alterations identified by proteomic analysis in human renal cell carcinoma
title_fullStr Metabolism-related enzyme alterations identified by proteomic analysis in human renal cell carcinoma
title_full_unstemmed Metabolism-related enzyme alterations identified by proteomic analysis in human renal cell carcinoma
title_short Metabolism-related enzyme alterations identified by proteomic analysis in human renal cell carcinoma
title_sort metabolism-related enzyme alterations identified by proteomic analysis in human renal cell carcinoma
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4790526/
https://www.ncbi.nlm.nih.gov/pubmed/27022288
http://dx.doi.org/10.2147/OTT.S91953
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