Identification of the Hydrogen Uptake Gene Cluster for Chemolithoautotrophic Growth and Symbiosis Hydrogen Uptake in Bradyrhizobium Diazoefficiens

The hydrogen uptake (Hup) system of Bradyrhizobium diazoefficiens recycles the H(2) released by nitrogenase in soybean nodule symbiosis, and is responsible for H(2)-dependent chemolithoautotrophic growth. The strain USDA110 has two hup gene clusters located outside (locus I) and inside (locus II) a...

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Detalles Bibliográficos
Autores principales: Masuda, Sachiko, Saito, Masaki, Sugawara, Chiaki, Itakura, Manabu, Eda, Shima, Minamisawa, Kiwamu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2016
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4791120/
https://www.ncbi.nlm.nih.gov/pubmed/26911707
http://dx.doi.org/10.1264/jsme2.ME15182
Descripción
Sumario:The hydrogen uptake (Hup) system of Bradyrhizobium diazoefficiens recycles the H(2) released by nitrogenase in soybean nodule symbiosis, and is responsible for H(2)-dependent chemolithoautotrophic growth. The strain USDA110 has two hup gene clusters located outside (locus I) and inside (locus II) a symbiosis island. Bacterial growth under H(2)-dependent chemolithoautotrophic conditions was markedly weaker and H(2) production by soybean nodules was markedly stronger for the mutant of hup locus I (ΔhupS(1)L(1)) than for the mutant of hup locus II (ΔhupS(2)L(2)). These results indicate that locus I is primarily responsible for Hup activity.

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