Identification of the Hydrogen Uptake Gene Cluster for Chemolithoautotrophic Growth and Symbiosis Hydrogen Uptake in Bradyrhizobium Diazoefficiens

The hydrogen uptake (Hup) system of Bradyrhizobium diazoefficiens recycles the H(2) released by nitrogenase in soybean nodule symbiosis, and is responsible for H(2)-dependent chemolithoautotrophic growth. The strain USDA110 has two hup gene clusters located outside (locus I) and inside (locus II) a...

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Autores principales: Masuda, Sachiko, Saito, Masaki, Sugawara, Chiaki, Itakura, Manabu, Eda, Shima, Minamisawa, Kiwamu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2016
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4791120/
https://www.ncbi.nlm.nih.gov/pubmed/26911707
http://dx.doi.org/10.1264/jsme2.ME15182
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author Masuda, Sachiko
Saito, Masaki
Sugawara, Chiaki
Itakura, Manabu
Eda, Shima
Minamisawa, Kiwamu
author_facet Masuda, Sachiko
Saito, Masaki
Sugawara, Chiaki
Itakura, Manabu
Eda, Shima
Minamisawa, Kiwamu
author_sort Masuda, Sachiko
collection PubMed
description The hydrogen uptake (Hup) system of Bradyrhizobium diazoefficiens recycles the H(2) released by nitrogenase in soybean nodule symbiosis, and is responsible for H(2)-dependent chemolithoautotrophic growth. The strain USDA110 has two hup gene clusters located outside (locus I) and inside (locus II) a symbiosis island. Bacterial growth under H(2)-dependent chemolithoautotrophic conditions was markedly weaker and H(2) production by soybean nodules was markedly stronger for the mutant of hup locus I (ΔhupS(1)L(1)) than for the mutant of hup locus II (ΔhupS(2)L(2)). These results indicate that locus I is primarily responsible for Hup activity.
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spelling pubmed-47911202016-03-21 Identification of the Hydrogen Uptake Gene Cluster for Chemolithoautotrophic Growth and Symbiosis Hydrogen Uptake in Bradyrhizobium Diazoefficiens Masuda, Sachiko Saito, Masaki Sugawara, Chiaki Itakura, Manabu Eda, Shima Minamisawa, Kiwamu Microbes Environ Short Communication The hydrogen uptake (Hup) system of Bradyrhizobium diazoefficiens recycles the H(2) released by nitrogenase in soybean nodule symbiosis, and is responsible for H(2)-dependent chemolithoautotrophic growth. The strain USDA110 has two hup gene clusters located outside (locus I) and inside (locus II) a symbiosis island. Bacterial growth under H(2)-dependent chemolithoautotrophic conditions was markedly weaker and H(2) production by soybean nodules was markedly stronger for the mutant of hup locus I (ΔhupS(1)L(1)) than for the mutant of hup locus II (ΔhupS(2)L(2)). These results indicate that locus I is primarily responsible for Hup activity. the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2016-03 2016-02-25 /pmc/articles/PMC4791120/ /pubmed/26911707 http://dx.doi.org/10.1264/jsme2.ME15182 Text en Copyright © 2016 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Masuda, Sachiko
Saito, Masaki
Sugawara, Chiaki
Itakura, Manabu
Eda, Shima
Minamisawa, Kiwamu
Identification of the Hydrogen Uptake Gene Cluster for Chemolithoautotrophic Growth and Symbiosis Hydrogen Uptake in Bradyrhizobium Diazoefficiens
title Identification of the Hydrogen Uptake Gene Cluster for Chemolithoautotrophic Growth and Symbiosis Hydrogen Uptake in Bradyrhizobium Diazoefficiens
title_full Identification of the Hydrogen Uptake Gene Cluster for Chemolithoautotrophic Growth and Symbiosis Hydrogen Uptake in Bradyrhizobium Diazoefficiens
title_fullStr Identification of the Hydrogen Uptake Gene Cluster for Chemolithoautotrophic Growth and Symbiosis Hydrogen Uptake in Bradyrhizobium Diazoefficiens
title_full_unstemmed Identification of the Hydrogen Uptake Gene Cluster for Chemolithoautotrophic Growth and Symbiosis Hydrogen Uptake in Bradyrhizobium Diazoefficiens
title_short Identification of the Hydrogen Uptake Gene Cluster for Chemolithoautotrophic Growth and Symbiosis Hydrogen Uptake in Bradyrhizobium Diazoefficiens
title_sort identification of the hydrogen uptake gene cluster for chemolithoautotrophic growth and symbiosis hydrogen uptake in bradyrhizobium diazoefficiens
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4791120/
https://www.ncbi.nlm.nih.gov/pubmed/26911707
http://dx.doi.org/10.1264/jsme2.ME15182
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