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Validation of a multicolor staining to monitor (phospho)STAT5 levels in regulatory T-cell subsets

BACKGROUND: Regulatory T cells (T(regs)) are key players in immune tolerance. They express the transcription factor FOXP3 and are dependent of the STAT5 signaling for their homeostasis. So far, the study of phosphorylated epitopes by flow cytometry required treating the cells with methanol, which is...

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Detalles Bibliográficos
Autores principales:	Ehx, Grégory, Hannon, Muriel, Beguin, Yves, Humblet-Baron, Stéphanie, Baron, Frédéric
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Impact Journals LLC 2015
Materias:	Research Paper: Immunology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4791230/ https://www.ncbi.nlm.nih.gov/pubmed/26657728

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