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Hepatitis B virus core antigen as a carrier for Chlamydia trachomatis MOMP multi-epitope peptide enhances protection against genital chlamydial infection

Chlamydia trachomatis (Ct) is the leading cause of sexually transmitted diseases worldwide. There is no safe and effective vaccine to control the spread of Ct. In development of Ct vaccine, selection of appropriate candidate antigens and an effective delivery system may be the main challenges. Multi...

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Autores principales: Jiang, Pengfei, Du, Wangqi, Xiong, Yirong, Lv, Yan, Feng, Juan, Zhu, Shanli, Xue, Xiangyang, Chen, Shao, Zhang, Lifang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4791232/
https://www.ncbi.nlm.nih.gov/pubmed/26657117
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author Jiang, Pengfei
Du, Wangqi
Xiong, Yirong
Lv, Yan
Feng, Juan
Zhu, Shanli
Xue, Xiangyang
Chen, Shao
Zhang, Lifang
author_facet Jiang, Pengfei
Du, Wangqi
Xiong, Yirong
Lv, Yan
Feng, Juan
Zhu, Shanli
Xue, Xiangyang
Chen, Shao
Zhang, Lifang
author_sort Jiang, Pengfei
collection PubMed
description Chlamydia trachomatis (Ct) is the leading cause of sexually transmitted diseases worldwide. There is no safe and effective vaccine to control the spread of Ct. In development of Ct vaccine, selection of appropriate candidate antigens and an effective delivery system may be the main challenges. Multi-epitope of major outer membrane protein (MOMPm) is the most suitable candidate for a Ct vaccine, while hepatitis B virus core antigen (HBcAg) has unique advantages as vaccine delivery system. Therefore, in this study, we evaluated the immunogenicity and protective immune response of a novel candidate vaccine in a murine model of chlamydial genital infection. This candidate vaccine comprises MOMPm peptide delivered with HBcAg. Our results of Ct-specific serum IgG and secretory IgA assay, cytokine assay, and cytotoxic T-lymphocyte assay revealed that immunogenicity of the candidate vaccine was much better than that of the corresponding synthetic MOMPm peptide. Furthermore, the protective effect of the candidate vaccine was also shown much better than that of the synthetic peptide by calculating the isolation of Chlamydia from vaginal swabs and histopathological analysis. Taken together, our results indicate that HBcAg carrying Ct MOMPm could be an effective immune prophylactic for chlamydial infection.
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spelling pubmed-47912322016-03-28 Hepatitis B virus core antigen as a carrier for Chlamydia trachomatis MOMP multi-epitope peptide enhances protection against genital chlamydial infection Jiang, Pengfei Du, Wangqi Xiong, Yirong Lv, Yan Feng, Juan Zhu, Shanli Xue, Xiangyang Chen, Shao Zhang, Lifang Oncotarget Research Paper: Immunology Chlamydia trachomatis (Ct) is the leading cause of sexually transmitted diseases worldwide. There is no safe and effective vaccine to control the spread of Ct. In development of Ct vaccine, selection of appropriate candidate antigens and an effective delivery system may be the main challenges. Multi-epitope of major outer membrane protein (MOMPm) is the most suitable candidate for a Ct vaccine, while hepatitis B virus core antigen (HBcAg) has unique advantages as vaccine delivery system. Therefore, in this study, we evaluated the immunogenicity and protective immune response of a novel candidate vaccine in a murine model of chlamydial genital infection. This candidate vaccine comprises MOMPm peptide delivered with HBcAg. Our results of Ct-specific serum IgG and secretory IgA assay, cytokine assay, and cytotoxic T-lymphocyte assay revealed that immunogenicity of the candidate vaccine was much better than that of the corresponding synthetic MOMPm peptide. Furthermore, the protective effect of the candidate vaccine was also shown much better than that of the synthetic peptide by calculating the isolation of Chlamydia from vaginal swabs and histopathological analysis. Taken together, our results indicate that HBcAg carrying Ct MOMPm could be an effective immune prophylactic for chlamydial infection. Impact Journals LLC 2015-12-09 /pmc/articles/PMC4791232/ /pubmed/26657117 Text en Copyright: © 2015 Jiang et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper: Immunology
Jiang, Pengfei
Du, Wangqi
Xiong, Yirong
Lv, Yan
Feng, Juan
Zhu, Shanli
Xue, Xiangyang
Chen, Shao
Zhang, Lifang
Hepatitis B virus core antigen as a carrier for Chlamydia trachomatis MOMP multi-epitope peptide enhances protection against genital chlamydial infection
title Hepatitis B virus core antigen as a carrier for Chlamydia trachomatis MOMP multi-epitope peptide enhances protection against genital chlamydial infection
title_full Hepatitis B virus core antigen as a carrier for Chlamydia trachomatis MOMP multi-epitope peptide enhances protection against genital chlamydial infection
title_fullStr Hepatitis B virus core antigen as a carrier for Chlamydia trachomatis MOMP multi-epitope peptide enhances protection against genital chlamydial infection
title_full_unstemmed Hepatitis B virus core antigen as a carrier for Chlamydia trachomatis MOMP multi-epitope peptide enhances protection against genital chlamydial infection
title_short Hepatitis B virus core antigen as a carrier for Chlamydia trachomatis MOMP multi-epitope peptide enhances protection against genital chlamydial infection
title_sort hepatitis b virus core antigen as a carrier for chlamydia trachomatis momp multi-epitope peptide enhances protection against genital chlamydial infection
topic Research Paper: Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4791232/
https://www.ncbi.nlm.nih.gov/pubmed/26657117
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