Cargando…

Ultrastructure of a Mobile Threadlike Tissue Floating in a Lymph Vessel

Observations of the primo vascular system (PVS) floating in lymph ducts were reported by various groups. There have been, however, no studies on the ultrastructure of the entire cross section of a primo vessel (PV) inside a lymph vessel with a transmission electron microscope (TEM). In the current s...

Descripción completa

Detalles Bibliográficos
Autores principales: Jung, Sharon Jiyoon, Gil, HyunJi, Kim, Dong-Hyun, Kim, Hong-Lim, Kim, Sungchul, Soh, Kwang-Sup
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4791497/
https://www.ncbi.nlm.nih.gov/pubmed/27034687
http://dx.doi.org/10.1155/2016/3064072
_version_ 1782421101626785792
author Jung, Sharon Jiyoon
Gil, HyunJi
Kim, Dong-Hyun
Kim, Hong-Lim
Kim, Sungchul
Soh, Kwang-Sup
author_facet Jung, Sharon Jiyoon
Gil, HyunJi
Kim, Dong-Hyun
Kim, Hong-Lim
Kim, Sungchul
Soh, Kwang-Sup
author_sort Jung, Sharon Jiyoon
collection PubMed
description Observations of the primo vascular system (PVS) floating in lymph ducts were reported by various groups. There have been, however, no studies on the ultrastructure of the entire cross section of a primo vessel (PV) inside a lymph vessel with a transmission electron microscope (TEM). In the current study we took the TEM images of a cross section of the PV inside a lymph vessel. We used the Alcian blue staining method for the finding of the target PV in a lymphatic vessel by injecting the dye into the inguinal lymph nodes. The stained PV was harvested together with the lymph vessel and some parts of the specimens were used for studying with optical microscopes. Some other parts were treated according to a standard protocol for TEM. As the results the TEM study revealed the loosely distributed collagen fibers with plenty of empty spaces and the lumens with the endothelial nuclei. It turned out to be very similar to the ultrastructure of the PVs observed on the surfaces of internal organs. It also showed how compactly the PV is surrounded with lymphocytes. In conclusion, the detailed morphological features like the distribution of fibers in the PV were revealed and shown to be similar to another kind of the PV on the surfaces of internal organs.
format Online
Article
Text
id pubmed-4791497
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-47914972016-03-31 Ultrastructure of a Mobile Threadlike Tissue Floating in a Lymph Vessel Jung, Sharon Jiyoon Gil, HyunJi Kim, Dong-Hyun Kim, Hong-Lim Kim, Sungchul Soh, Kwang-Sup Evid Based Complement Alternat Med Research Article Observations of the primo vascular system (PVS) floating in lymph ducts were reported by various groups. There have been, however, no studies on the ultrastructure of the entire cross section of a primo vessel (PV) inside a lymph vessel with a transmission electron microscope (TEM). In the current study we took the TEM images of a cross section of the PV inside a lymph vessel. We used the Alcian blue staining method for the finding of the target PV in a lymphatic vessel by injecting the dye into the inguinal lymph nodes. The stained PV was harvested together with the lymph vessel and some parts of the specimens were used for studying with optical microscopes. Some other parts were treated according to a standard protocol for TEM. As the results the TEM study revealed the loosely distributed collagen fibers with plenty of empty spaces and the lumens with the endothelial nuclei. It turned out to be very similar to the ultrastructure of the PVs observed on the surfaces of internal organs. It also showed how compactly the PV is surrounded with lymphocytes. In conclusion, the detailed morphological features like the distribution of fibers in the PV were revealed and shown to be similar to another kind of the PV on the surfaces of internal organs. Hindawi Publishing Corporation 2016 2016-03-01 /pmc/articles/PMC4791497/ /pubmed/27034687 http://dx.doi.org/10.1155/2016/3064072 Text en Copyright © 2016 Sharon Jiyoon Jung et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jung, Sharon Jiyoon
Gil, HyunJi
Kim, Dong-Hyun
Kim, Hong-Lim
Kim, Sungchul
Soh, Kwang-Sup
Ultrastructure of a Mobile Threadlike Tissue Floating in a Lymph Vessel
title Ultrastructure of a Mobile Threadlike Tissue Floating in a Lymph Vessel
title_full Ultrastructure of a Mobile Threadlike Tissue Floating in a Lymph Vessel
title_fullStr Ultrastructure of a Mobile Threadlike Tissue Floating in a Lymph Vessel
title_full_unstemmed Ultrastructure of a Mobile Threadlike Tissue Floating in a Lymph Vessel
title_short Ultrastructure of a Mobile Threadlike Tissue Floating in a Lymph Vessel
title_sort ultrastructure of a mobile threadlike tissue floating in a lymph vessel
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4791497/
https://www.ncbi.nlm.nih.gov/pubmed/27034687
http://dx.doi.org/10.1155/2016/3064072
work_keys_str_mv AT jungsharonjiyoon ultrastructureofamobilethreadliketissuefloatinginalymphvessel
AT gilhyunji ultrastructureofamobilethreadliketissuefloatinginalymphvessel
AT kimdonghyun ultrastructureofamobilethreadliketissuefloatinginalymphvessel
AT kimhonglim ultrastructureofamobilethreadliketissuefloatinginalymphvessel
AT kimsungchul ultrastructureofamobilethreadliketissuefloatinginalymphvessel
AT sohkwangsup ultrastructureofamobilethreadliketissuefloatinginalymphvessel