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Essential Oil from the Resin of Protium heptaphyllum: Chemical Composition, Cytotoxicity, Antimicrobial Activity, and Antimutagenicity

BACKGROUND: Protium heptaphyllum (Aubl.) March is popularly used as an analgesic and anti-inflammatory agent. OBJECTIVE: This study aimed to evaluate the chemical composition of P. heptaphyllum essential oil, its cytotoxicity in a breast cancer cell line (MCF-7), antimicrobial activity, and its anti...

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Autores principales: de Lima, Ewelyne Miranda, Cazelli, Didley Sâmia Paiva, Pinto, Fernanda Endringer, Mazuco, Renata Alves, Kalil, Ieda Carneiro, Lenz, Dominik, Scherer, Rodrigo, de Andrade, Tadeu Uggere, Endringer, Denise Coutinho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4791998/
https://www.ncbi.nlm.nih.gov/pubmed/27041857
http://dx.doi.org/10.4103/0973-1296.176113
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author de Lima, Ewelyne Miranda
Cazelli, Didley Sâmia Paiva
Pinto, Fernanda Endringer
Mazuco, Renata Alves
Kalil, Ieda Carneiro
Lenz, Dominik
Scherer, Rodrigo
de Andrade, Tadeu Uggere
Endringer, Denise Coutinho
author_facet de Lima, Ewelyne Miranda
Cazelli, Didley Sâmia Paiva
Pinto, Fernanda Endringer
Mazuco, Renata Alves
Kalil, Ieda Carneiro
Lenz, Dominik
Scherer, Rodrigo
de Andrade, Tadeu Uggere
Endringer, Denise Coutinho
author_sort de Lima, Ewelyne Miranda
collection PubMed
description BACKGROUND: Protium heptaphyllum (Aubl.) March is popularly used as an analgesic and anti-inflammatory agent. OBJECTIVE: This study aimed to evaluate the chemical composition of P. heptaphyllum essential oil, its cytotoxicity in a breast cancer cell line (MCF-7), antimicrobial activity, and its antimutagenicity in vivo. MATERIALS AND METHODS: The chemical composition of the essential oil collected in three 3 years was determined by gas chromatography-mass spectrometry. The cytotoxicity was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Annexin V conjugated with fluorescein isothiocyanate, caspase-3, and tumor necrosis factor-alpha (TNF-α) assays were performed to evaluate apoptosis and inflammatory events. The antimutagenic activity at doses of 25, 50, and 100 mg/kg was determined using a micronucleus test in murine bone marrow. RESULTS: The essential oil showed a predominance of monoterpene compounds, being the terpinolene, p-cymene-8-ol, and p-cymene, present in the essential oil extracted in the 3 years. The essential oil showed a protection against cyclophosphamide-induced genotoxicity, and the cytotoxicity index polychromatic erythrocytes/normochromatic erythrocytes ratio in animals treated with oil at all doses (1.34 ± 0.33; 1.15 ± 0.1; 1.11 ± 0.13) did not differ from the negative control animal (1.31 ± 0.33), but from the cyclophosphamide group (0.61 ± 0.12). Cytotoxicity, at a concentration of 40.0 μg/mL, and antimicrobial activity were not observed for the essential oil (minimum inhibitory concentration ≥0.5 mg/mL). The essential oil did not change the levels of caspase-3 in the TNF-α level. CONCLUSION: The essential oil showed antimutagenic activity due to its chemical composition. SUMMARY: Terpinolene, p-cymene-8-ol, and p-cymene are the main constituents of the essential oil of P. heptaphyllum collected within 3-years. The essential oil of P. heptaphyllum did not show antimicrobial activity (MIC >0.5 mg/mL) against E. coli, S. aureus, E. faecalis, and C. albicans. The essential oil of P. heptaphyllum has activity against S. mutans (MIC = 0.5 mg/mL). The essential oil showed a protection against cyclophosphamide-induced genotoxicity in the micronuclei assay. Abbreviations used: GC–MS: Gas Chromatography–Mass Spectrometry, MTT: 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Annexin V-FITC: Annexin V conjugated with fluorescein isothiocyanate, TNF-α: Tumor necrosis factor alpha, MIC: Minimum Inhibitory Concentration
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spelling pubmed-47919982016-04-01 Essential Oil from the Resin of Protium heptaphyllum: Chemical Composition, Cytotoxicity, Antimicrobial Activity, and Antimutagenicity de Lima, Ewelyne Miranda Cazelli, Didley Sâmia Paiva Pinto, Fernanda Endringer Mazuco, Renata Alves Kalil, Ieda Carneiro Lenz, Dominik Scherer, Rodrigo de Andrade, Tadeu Uggere Endringer, Denise Coutinho Pharmacogn Mag Original Article BACKGROUND: Protium heptaphyllum (Aubl.) March is popularly used as an analgesic and anti-inflammatory agent. OBJECTIVE: This study aimed to evaluate the chemical composition of P. heptaphyllum essential oil, its cytotoxicity in a breast cancer cell line (MCF-7), antimicrobial activity, and its antimutagenicity in vivo. MATERIALS AND METHODS: The chemical composition of the essential oil collected in three 3 years was determined by gas chromatography-mass spectrometry. The cytotoxicity was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Annexin V conjugated with fluorescein isothiocyanate, caspase-3, and tumor necrosis factor-alpha (TNF-α) assays were performed to evaluate apoptosis and inflammatory events. The antimutagenic activity at doses of 25, 50, and 100 mg/kg was determined using a micronucleus test in murine bone marrow. RESULTS: The essential oil showed a predominance of monoterpene compounds, being the terpinolene, p-cymene-8-ol, and p-cymene, present in the essential oil extracted in the 3 years. The essential oil showed a protection against cyclophosphamide-induced genotoxicity, and the cytotoxicity index polychromatic erythrocytes/normochromatic erythrocytes ratio in animals treated with oil at all doses (1.34 ± 0.33; 1.15 ± 0.1; 1.11 ± 0.13) did not differ from the negative control animal (1.31 ± 0.33), but from the cyclophosphamide group (0.61 ± 0.12). Cytotoxicity, at a concentration of 40.0 μg/mL, and antimicrobial activity were not observed for the essential oil (minimum inhibitory concentration ≥0.5 mg/mL). The essential oil did not change the levels of caspase-3 in the TNF-α level. CONCLUSION: The essential oil showed antimutagenic activity due to its chemical composition. SUMMARY: Terpinolene, p-cymene-8-ol, and p-cymene are the main constituents of the essential oil of P. heptaphyllum collected within 3-years. The essential oil of P. heptaphyllum did not show antimicrobial activity (MIC >0.5 mg/mL) against E. coli, S. aureus, E. faecalis, and C. albicans. The essential oil of P. heptaphyllum has activity against S. mutans (MIC = 0.5 mg/mL). The essential oil showed a protection against cyclophosphamide-induced genotoxicity in the micronuclei assay. Abbreviations used: GC–MS: Gas Chromatography–Mass Spectrometry, MTT: 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Annexin V-FITC: Annexin V conjugated with fluorescein isothiocyanate, TNF-α: Tumor necrosis factor alpha, MIC: Minimum Inhibitory Concentration Medknow Publications & Media Pvt Ltd 2016-01 /pmc/articles/PMC4791998/ /pubmed/27041857 http://dx.doi.org/10.4103/0973-1296.176113 Text en Copyright: © Pharmacognosy Magazine http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
de Lima, Ewelyne Miranda
Cazelli, Didley Sâmia Paiva
Pinto, Fernanda Endringer
Mazuco, Renata Alves
Kalil, Ieda Carneiro
Lenz, Dominik
Scherer, Rodrigo
de Andrade, Tadeu Uggere
Endringer, Denise Coutinho
Essential Oil from the Resin of Protium heptaphyllum: Chemical Composition, Cytotoxicity, Antimicrobial Activity, and Antimutagenicity
title Essential Oil from the Resin of Protium heptaphyllum: Chemical Composition, Cytotoxicity, Antimicrobial Activity, and Antimutagenicity
title_full Essential Oil from the Resin of Protium heptaphyllum: Chemical Composition, Cytotoxicity, Antimicrobial Activity, and Antimutagenicity
title_fullStr Essential Oil from the Resin of Protium heptaphyllum: Chemical Composition, Cytotoxicity, Antimicrobial Activity, and Antimutagenicity
title_full_unstemmed Essential Oil from the Resin of Protium heptaphyllum: Chemical Composition, Cytotoxicity, Antimicrobial Activity, and Antimutagenicity
title_short Essential Oil from the Resin of Protium heptaphyllum: Chemical Composition, Cytotoxicity, Antimicrobial Activity, and Antimutagenicity
title_sort essential oil from the resin of protium heptaphyllum: chemical composition, cytotoxicity, antimicrobial activity, and antimutagenicity
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4791998/
https://www.ncbi.nlm.nih.gov/pubmed/27041857
http://dx.doi.org/10.4103/0973-1296.176113
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