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Sensitivity of human pleural mesothelioma to oncolytic measles virus depends on defects of the type I interferon response
Attenuated measles virus (MV) is currently being evaluated as an oncolytic virus in clinical trials and could represent a new therapeutic approach for malignant pleural mesothelioma (MPM). Herein, we screened the sensitivity to MV infection and replication of twenty-two human MPM cell lines and some...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4792599/ https://www.ncbi.nlm.nih.gov/pubmed/26539644 |
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author | Achard, Carole Boisgerault, Nicolas Delaunay, Tiphaine Roulois, David Nedellec, Steven Royer, Pierre-Joseph Pain, Mallory Combredet, Chantal Mesel-Lemoine, Mariana Cellerin, Laurent Magnan, Antoine Tangy, Frédéric Grégoire, Marc Fonteneau, Jean-François |
author_facet | Achard, Carole Boisgerault, Nicolas Delaunay, Tiphaine Roulois, David Nedellec, Steven Royer, Pierre-Joseph Pain, Mallory Combredet, Chantal Mesel-Lemoine, Mariana Cellerin, Laurent Magnan, Antoine Tangy, Frédéric Grégoire, Marc Fonteneau, Jean-François |
author_sort | Achard, Carole |
collection | PubMed |
description | Attenuated measles virus (MV) is currently being evaluated as an oncolytic virus in clinical trials and could represent a new therapeutic approach for malignant pleural mesothelioma (MPM). Herein, we screened the sensitivity to MV infection and replication of twenty-two human MPM cell lines and some healthy primary cells. We show that MV replicates in fifteen of the twenty-two MPM cell lines. Despite overexpression of CD46 by a majority of MPM cell lines compared to healthy cells, we found that the sensitivity to MV replication did not correlate with this overexpression. We then evaluated the antiviral type I interferon (IFN) responses of MPM cell lines and healthy cells. We found that healthy cells and the seven insensitive MPM cell lines developed a type I IFN response in presence of the virus, thereby inhibiting replication. In contrast, eleven of the fifteen sensitive MPM cell lines were unable to develop a complete type I IFN response in presence of MV. Finally, we show that addition of type I IFN onto MV sensitive tumor cell lines inhibits replication. These results demonstrate that defects in type I IFN response are frequent in MPM and that MV takes advantage of these defects to exert oncolytic activity. |
format | Online Article Text |
id | pubmed-4792599 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-47925992016-03-29 Sensitivity of human pleural mesothelioma to oncolytic measles virus depends on defects of the type I interferon response Achard, Carole Boisgerault, Nicolas Delaunay, Tiphaine Roulois, David Nedellec, Steven Royer, Pierre-Joseph Pain, Mallory Combredet, Chantal Mesel-Lemoine, Mariana Cellerin, Laurent Magnan, Antoine Tangy, Frédéric Grégoire, Marc Fonteneau, Jean-François Oncotarget Research Paper Attenuated measles virus (MV) is currently being evaluated as an oncolytic virus in clinical trials and could represent a new therapeutic approach for malignant pleural mesothelioma (MPM). Herein, we screened the sensitivity to MV infection and replication of twenty-two human MPM cell lines and some healthy primary cells. We show that MV replicates in fifteen of the twenty-two MPM cell lines. Despite overexpression of CD46 by a majority of MPM cell lines compared to healthy cells, we found that the sensitivity to MV replication did not correlate with this overexpression. We then evaluated the antiviral type I interferon (IFN) responses of MPM cell lines and healthy cells. We found that healthy cells and the seven insensitive MPM cell lines developed a type I IFN response in presence of the virus, thereby inhibiting replication. In contrast, eleven of the fifteen sensitive MPM cell lines were unable to develop a complete type I IFN response in presence of MV. Finally, we show that addition of type I IFN onto MV sensitive tumor cell lines inhibits replication. These results demonstrate that defects in type I IFN response are frequent in MPM and that MV takes advantage of these defects to exert oncolytic activity. Impact Journals LLC 2015-11-02 /pmc/articles/PMC4792599/ /pubmed/26539644 Text en Copyright: © 2015 Achard et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Achard, Carole Boisgerault, Nicolas Delaunay, Tiphaine Roulois, David Nedellec, Steven Royer, Pierre-Joseph Pain, Mallory Combredet, Chantal Mesel-Lemoine, Mariana Cellerin, Laurent Magnan, Antoine Tangy, Frédéric Grégoire, Marc Fonteneau, Jean-François Sensitivity of human pleural mesothelioma to oncolytic measles virus depends on defects of the type I interferon response |
title | Sensitivity of human pleural mesothelioma to oncolytic measles virus depends on defects of the type I interferon response |
title_full | Sensitivity of human pleural mesothelioma to oncolytic measles virus depends on defects of the type I interferon response |
title_fullStr | Sensitivity of human pleural mesothelioma to oncolytic measles virus depends on defects of the type I interferon response |
title_full_unstemmed | Sensitivity of human pleural mesothelioma to oncolytic measles virus depends on defects of the type I interferon response |
title_short | Sensitivity of human pleural mesothelioma to oncolytic measles virus depends on defects of the type I interferon response |
title_sort | sensitivity of human pleural mesothelioma to oncolytic measles virus depends on defects of the type i interferon response |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4792599/ https://www.ncbi.nlm.nih.gov/pubmed/26539644 |
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