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A subunit of the oligosaccharyltransferase complex is required for interspecific gametophyte recognition in Arabidopsis

Species-specific gamete recognition is a key premise to ensure reproductive success and the maintenance of species boundaries. During plant pollen tube (PT) reception, gametophyte interactions likely allow the species-specific recognition of signals from the PT (male gametophyte) by the embryo sac (...

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Autores principales: Müller, Lena M., Lindner, Heike, Pires, Nuno D., Gagliardini, Valeria, Grossniklaus, Ueli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4792959/
https://www.ncbi.nlm.nih.gov/pubmed/26964640
http://dx.doi.org/10.1038/ncomms10826
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author Müller, Lena M.
Lindner, Heike
Pires, Nuno D.
Gagliardini, Valeria
Grossniklaus, Ueli
author_facet Müller, Lena M.
Lindner, Heike
Pires, Nuno D.
Gagliardini, Valeria
Grossniklaus, Ueli
author_sort Müller, Lena M.
collection PubMed
description Species-specific gamete recognition is a key premise to ensure reproductive success and the maintenance of species boundaries. During plant pollen tube (PT) reception, gametophyte interactions likely allow the species-specific recognition of signals from the PT (male gametophyte) by the embryo sac (female gametophyte), resulting in PT rupture, sperm release, and double fertilization. This process is impaired in interspecific crosses between Arabidopsis thaliana and related species, leading to PT overgrowth and a failure to deliver the sperm cells. Here we show that ARTUMES (ARU) specifically regulates the recognition of interspecific PTs in A. thaliana. ARU, identified in a genome-wide association study (GWAS), exclusively influences interspecific—but not intraspecific—gametophyte interactions. ARU encodes the OST3/6 subunit of the oligosaccharyltransferase complex conferring protein N-glycosylation. Our results suggest that glycosylation patterns of cell surface proteins may represent an important mechanism of gametophyte recognition and thus speciation.
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spelling pubmed-47929592016-03-21 A subunit of the oligosaccharyltransferase complex is required for interspecific gametophyte recognition in Arabidopsis Müller, Lena M. Lindner, Heike Pires, Nuno D. Gagliardini, Valeria Grossniklaus, Ueli Nat Commun Article Species-specific gamete recognition is a key premise to ensure reproductive success and the maintenance of species boundaries. During plant pollen tube (PT) reception, gametophyte interactions likely allow the species-specific recognition of signals from the PT (male gametophyte) by the embryo sac (female gametophyte), resulting in PT rupture, sperm release, and double fertilization. This process is impaired in interspecific crosses between Arabidopsis thaliana and related species, leading to PT overgrowth and a failure to deliver the sperm cells. Here we show that ARTUMES (ARU) specifically regulates the recognition of interspecific PTs in A. thaliana. ARU, identified in a genome-wide association study (GWAS), exclusively influences interspecific—but not intraspecific—gametophyte interactions. ARU encodes the OST3/6 subunit of the oligosaccharyltransferase complex conferring protein N-glycosylation. Our results suggest that glycosylation patterns of cell surface proteins may represent an important mechanism of gametophyte recognition and thus speciation. Nature Publishing Group 2016-03-11 /pmc/articles/PMC4792959/ /pubmed/26964640 http://dx.doi.org/10.1038/ncomms10826 Text en Copyright © 2016, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Müller, Lena M.
Lindner, Heike
Pires, Nuno D.
Gagliardini, Valeria
Grossniklaus, Ueli
A subunit of the oligosaccharyltransferase complex is required for interspecific gametophyte recognition in Arabidopsis
title A subunit of the oligosaccharyltransferase complex is required for interspecific gametophyte recognition in Arabidopsis
title_full A subunit of the oligosaccharyltransferase complex is required for interspecific gametophyte recognition in Arabidopsis
title_fullStr A subunit of the oligosaccharyltransferase complex is required for interspecific gametophyte recognition in Arabidopsis
title_full_unstemmed A subunit of the oligosaccharyltransferase complex is required for interspecific gametophyte recognition in Arabidopsis
title_short A subunit of the oligosaccharyltransferase complex is required for interspecific gametophyte recognition in Arabidopsis
title_sort subunit of the oligosaccharyltransferase complex is required for interspecific gametophyte recognition in arabidopsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4792959/
https://www.ncbi.nlm.nih.gov/pubmed/26964640
http://dx.doi.org/10.1038/ncomms10826
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