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The Effect of Prolonged Culture of Chromosomally Abnormal Human Embryos on The Rate of Diploid Cells

BACKGROUND: A decrease in aneuploidy rate following a prolonged co-culture of human blastocysts has been reported. As co-culture is not routinely used in assisted reproductive technology, the present study aimed to evaluate the effect of the prolonged single culture on the rate of diploid cells in h...

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Autores principales: Bazrgar, Masood, Gourabi, Hamid, Eftekhari-Yazdi, Poopak, Vazirinasab, Hamed, Fakhri, Mostafa, Hassani, Fatemeh, Chehrazi, Mohamad, Valojerdi, Mojtaba Rezazadeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4793179/
https://www.ncbi.nlm.nih.gov/pubmed/26985346
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author Bazrgar, Masood
Gourabi, Hamid
Eftekhari-Yazdi, Poopak
Vazirinasab, Hamed
Fakhri, Mostafa
Hassani, Fatemeh
Chehrazi, Mohamad
Valojerdi, Mojtaba Rezazadeh
author_facet Bazrgar, Masood
Gourabi, Hamid
Eftekhari-Yazdi, Poopak
Vazirinasab, Hamed
Fakhri, Mostafa
Hassani, Fatemeh
Chehrazi, Mohamad
Valojerdi, Mojtaba Rezazadeh
author_sort Bazrgar, Masood
collection PubMed
description BACKGROUND: A decrease in aneuploidy rate following a prolonged co-culture of human blastocysts has been reported. As co-culture is not routinely used in assisted reproductive technology, the present study aimed to evaluate the effect of the prolonged single culture on the rate of diploid cells in human embryos with aneuploidies. MATERIALS AND METHODS: In this cohort study, we used fluorescence in situ hybridi- zation (FISH) to reanalyze surplus blastocysts undergoing preimplantation genetic diagnosis (PGD) on day 3 postfertilization. They were randomly studied on days 6 or 7 following fertilization. RESULTS: Of the 30 analyzed blastocysts, mosaicism was observed in 26(86.6%), while 2(6.7%) were diploid, and 2(6.7%) were triploid. Of those with mosaicism, 23(88.5%) were determined to be diploid-aneuploid and 3(11.5%) were aneuploid mosaic. The total frequency of embryos with more than 50% diploid cells was 33.3% that was lower on day 7 in comparison with the related value on day 6 (P<0.05); however, there were no differences when the embryos were classified according to maternal age, blastocyst developmental stage, total cell number on day 3, and embryo quality. CONCLUSION: Although mosaicism is frequently observed in blastocysts, the prolonged single culture of blastocysts does not seem to increase the rate of normal cells.
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spelling pubmed-47931792016-03-16 The Effect of Prolonged Culture of Chromosomally Abnormal Human Embryos on The Rate of Diploid Cells Bazrgar, Masood Gourabi, Hamid Eftekhari-Yazdi, Poopak Vazirinasab, Hamed Fakhri, Mostafa Hassani, Fatemeh Chehrazi, Mohamad Valojerdi, Mojtaba Rezazadeh Int J Fertil Steril Original Article BACKGROUND: A decrease in aneuploidy rate following a prolonged co-culture of human blastocysts has been reported. As co-culture is not routinely used in assisted reproductive technology, the present study aimed to evaluate the effect of the prolonged single culture on the rate of diploid cells in human embryos with aneuploidies. MATERIALS AND METHODS: In this cohort study, we used fluorescence in situ hybridi- zation (FISH) to reanalyze surplus blastocysts undergoing preimplantation genetic diagnosis (PGD) on day 3 postfertilization. They were randomly studied on days 6 or 7 following fertilization. RESULTS: Of the 30 analyzed blastocysts, mosaicism was observed in 26(86.6%), while 2(6.7%) were diploid, and 2(6.7%) were triploid. Of those with mosaicism, 23(88.5%) were determined to be diploid-aneuploid and 3(11.5%) were aneuploid mosaic. The total frequency of embryos with more than 50% diploid cells was 33.3% that was lower on day 7 in comparison with the related value on day 6 (P<0.05); however, there were no differences when the embryos were classified according to maternal age, blastocyst developmental stage, total cell number on day 3, and embryo quality. CONCLUSION: Although mosaicism is frequently observed in blastocysts, the prolonged single culture of blastocysts does not seem to increase the rate of normal cells. Royan Institute 2016 2015-12-23 /pmc/articles/PMC4793179/ /pubmed/26985346 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Bazrgar, Masood
Gourabi, Hamid
Eftekhari-Yazdi, Poopak
Vazirinasab, Hamed
Fakhri, Mostafa
Hassani, Fatemeh
Chehrazi, Mohamad
Valojerdi, Mojtaba Rezazadeh
The Effect of Prolonged Culture of Chromosomally Abnormal Human Embryos on The Rate of Diploid Cells
title The Effect of Prolonged Culture of Chromosomally Abnormal Human Embryos on The Rate of Diploid Cells
title_full The Effect of Prolonged Culture of Chromosomally Abnormal Human Embryos on The Rate of Diploid Cells
title_fullStr The Effect of Prolonged Culture of Chromosomally Abnormal Human Embryos on The Rate of Diploid Cells
title_full_unstemmed The Effect of Prolonged Culture of Chromosomally Abnormal Human Embryos on The Rate of Diploid Cells
title_short The Effect of Prolonged Culture of Chromosomally Abnormal Human Embryos on The Rate of Diploid Cells
title_sort effect of prolonged culture of chromosomally abnormal human embryos on the rate of diploid cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4793179/
https://www.ncbi.nlm.nih.gov/pubmed/26985346
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