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The Effect of Prolonged Culture of Chromosomally Abnormal Human Embryos on The Rate of Diploid Cells
BACKGROUND: A decrease in aneuploidy rate following a prolonged co-culture of human blastocysts has been reported. As co-culture is not routinely used in assisted reproductive technology, the present study aimed to evaluate the effect of the prolonged single culture on the rate of diploid cells in h...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Royan Institute
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4793179/ https://www.ncbi.nlm.nih.gov/pubmed/26985346 |
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author | Bazrgar, Masood Gourabi, Hamid Eftekhari-Yazdi, Poopak Vazirinasab, Hamed Fakhri, Mostafa Hassani, Fatemeh Chehrazi, Mohamad Valojerdi, Mojtaba Rezazadeh |
author_facet | Bazrgar, Masood Gourabi, Hamid Eftekhari-Yazdi, Poopak Vazirinasab, Hamed Fakhri, Mostafa Hassani, Fatemeh Chehrazi, Mohamad Valojerdi, Mojtaba Rezazadeh |
author_sort | Bazrgar, Masood |
collection | PubMed |
description | BACKGROUND: A decrease in aneuploidy rate following a prolonged co-culture of human blastocysts has been reported. As co-culture is not routinely used in assisted reproductive technology, the present study aimed to evaluate the effect of the prolonged single culture on the rate of diploid cells in human embryos with aneuploidies. MATERIALS AND METHODS: In this cohort study, we used fluorescence in situ hybridi- zation (FISH) to reanalyze surplus blastocysts undergoing preimplantation genetic diagnosis (PGD) on day 3 postfertilization. They were randomly studied on days 6 or 7 following fertilization. RESULTS: Of the 30 analyzed blastocysts, mosaicism was observed in 26(86.6%), while 2(6.7%) were diploid, and 2(6.7%) were triploid. Of those with mosaicism, 23(88.5%) were determined to be diploid-aneuploid and 3(11.5%) were aneuploid mosaic. The total frequency of embryos with more than 50% diploid cells was 33.3% that was lower on day 7 in comparison with the related value on day 6 (P<0.05); however, there were no differences when the embryos were classified according to maternal age, blastocyst developmental stage, total cell number on day 3, and embryo quality. CONCLUSION: Although mosaicism is frequently observed in blastocysts, the prolonged single culture of blastocysts does not seem to increase the rate of normal cells. |
format | Online Article Text |
id | pubmed-4793179 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Royan Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-47931792016-03-16 The Effect of Prolonged Culture of Chromosomally Abnormal Human Embryos on The Rate of Diploid Cells Bazrgar, Masood Gourabi, Hamid Eftekhari-Yazdi, Poopak Vazirinasab, Hamed Fakhri, Mostafa Hassani, Fatemeh Chehrazi, Mohamad Valojerdi, Mojtaba Rezazadeh Int J Fertil Steril Original Article BACKGROUND: A decrease in aneuploidy rate following a prolonged co-culture of human blastocysts has been reported. As co-culture is not routinely used in assisted reproductive technology, the present study aimed to evaluate the effect of the prolonged single culture on the rate of diploid cells in human embryos with aneuploidies. MATERIALS AND METHODS: In this cohort study, we used fluorescence in situ hybridi- zation (FISH) to reanalyze surplus blastocysts undergoing preimplantation genetic diagnosis (PGD) on day 3 postfertilization. They were randomly studied on days 6 or 7 following fertilization. RESULTS: Of the 30 analyzed blastocysts, mosaicism was observed in 26(86.6%), while 2(6.7%) were diploid, and 2(6.7%) were triploid. Of those with mosaicism, 23(88.5%) were determined to be diploid-aneuploid and 3(11.5%) were aneuploid mosaic. The total frequency of embryos with more than 50% diploid cells was 33.3% that was lower on day 7 in comparison with the related value on day 6 (P<0.05); however, there were no differences when the embryos were classified according to maternal age, blastocyst developmental stage, total cell number on day 3, and embryo quality. CONCLUSION: Although mosaicism is frequently observed in blastocysts, the prolonged single culture of blastocysts does not seem to increase the rate of normal cells. Royan Institute 2016 2015-12-23 /pmc/articles/PMC4793179/ /pubmed/26985346 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Bazrgar, Masood Gourabi, Hamid Eftekhari-Yazdi, Poopak Vazirinasab, Hamed Fakhri, Mostafa Hassani, Fatemeh Chehrazi, Mohamad Valojerdi, Mojtaba Rezazadeh The Effect of Prolonged Culture of Chromosomally Abnormal Human Embryos on The Rate of Diploid Cells |
title | The Effect of Prolonged Culture of Chromosomally
Abnormal Human Embryos on The Rate
of Diploid Cells |
title_full | The Effect of Prolonged Culture of Chromosomally
Abnormal Human Embryos on The Rate
of Diploid Cells |
title_fullStr | The Effect of Prolonged Culture of Chromosomally
Abnormal Human Embryos on The Rate
of Diploid Cells |
title_full_unstemmed | The Effect of Prolonged Culture of Chromosomally
Abnormal Human Embryos on The Rate
of Diploid Cells |
title_short | The Effect of Prolonged Culture of Chromosomally
Abnormal Human Embryos on The Rate
of Diploid Cells |
title_sort | effect of prolonged culture of chromosomally
abnormal human embryos on the rate
of diploid cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4793179/ https://www.ncbi.nlm.nih.gov/pubmed/26985346 |
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