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Mycobacterial escape from macrophage phagosomes to the cytoplasm represents an alternate adaptation mechanism
Survival of Mycobacterium tuberculosis (Mtb) within the host macrophage is mediated through pathogen-dependent inhibition of phagosome-lysosome fusion, which enables bacteria to persist within the immature phagosomal compartment. By employing ultrastructural examination of different field isolates s...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4793295/ https://www.ncbi.nlm.nih.gov/pubmed/26980157 http://dx.doi.org/10.1038/srep23089 |
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author | Jamwal, Shilpa V. Mehrotra, Parul Singh, Archana Siddiqui, Zaved Basu, Atanu Rao, Kanury V.S. |
author_facet | Jamwal, Shilpa V. Mehrotra, Parul Singh, Archana Siddiqui, Zaved Basu, Atanu Rao, Kanury V.S. |
author_sort | Jamwal, Shilpa V. |
collection | PubMed |
description | Survival of Mycobacterium tuberculosis (Mtb) within the host macrophage is mediated through pathogen-dependent inhibition of phagosome-lysosome fusion, which enables bacteria to persist within the immature phagosomal compartment. By employing ultrastructural examination of different field isolates supported by biochemical analysis, we found that some of the Mtb strains were in fact poorly adapted for subsistence within endocytic vesicles of infected macrophages. Instead, through a mechanism involving activation of host cytosolic phospholipase A(2), these bacteria rapidly escaped from phagosomes, and established residence in the cytoplasm of the host cell. Interestingly, by facilitating an enhanced suppression of host cellular autophagy, this translocation served as an alternate virulence acquisition mechanism. Thus, our studies reveal plasticity in the adaptation strategies employed by Mtb, for survival in the host macrophage. |
format | Online Article Text |
id | pubmed-4793295 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-47932952016-03-17 Mycobacterial escape from macrophage phagosomes to the cytoplasm represents an alternate adaptation mechanism Jamwal, Shilpa V. Mehrotra, Parul Singh, Archana Siddiqui, Zaved Basu, Atanu Rao, Kanury V.S. Sci Rep Article Survival of Mycobacterium tuberculosis (Mtb) within the host macrophage is mediated through pathogen-dependent inhibition of phagosome-lysosome fusion, which enables bacteria to persist within the immature phagosomal compartment. By employing ultrastructural examination of different field isolates supported by biochemical analysis, we found that some of the Mtb strains were in fact poorly adapted for subsistence within endocytic vesicles of infected macrophages. Instead, through a mechanism involving activation of host cytosolic phospholipase A(2), these bacteria rapidly escaped from phagosomes, and established residence in the cytoplasm of the host cell. Interestingly, by facilitating an enhanced suppression of host cellular autophagy, this translocation served as an alternate virulence acquisition mechanism. Thus, our studies reveal plasticity in the adaptation strategies employed by Mtb, for survival in the host macrophage. Nature Publishing Group 2016-03-16 /pmc/articles/PMC4793295/ /pubmed/26980157 http://dx.doi.org/10.1038/srep23089 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Jamwal, Shilpa V. Mehrotra, Parul Singh, Archana Siddiqui, Zaved Basu, Atanu Rao, Kanury V.S. Mycobacterial escape from macrophage phagosomes to the cytoplasm represents an alternate adaptation mechanism |
title | Mycobacterial escape from macrophage phagosomes to the cytoplasm represents an alternate adaptation mechanism |
title_full | Mycobacterial escape from macrophage phagosomes to the cytoplasm represents an alternate adaptation mechanism |
title_fullStr | Mycobacterial escape from macrophage phagosomes to the cytoplasm represents an alternate adaptation mechanism |
title_full_unstemmed | Mycobacterial escape from macrophage phagosomes to the cytoplasm represents an alternate adaptation mechanism |
title_short | Mycobacterial escape from macrophage phagosomes to the cytoplasm represents an alternate adaptation mechanism |
title_sort | mycobacterial escape from macrophage phagosomes to the cytoplasm represents an alternate adaptation mechanism |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4793295/ https://www.ncbi.nlm.nih.gov/pubmed/26980157 http://dx.doi.org/10.1038/srep23089 |
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