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Micro-computed tomography scan and virtual histological slide data for the land planarian Obama otavioi (Platyhelminthes)
BACKGROUND: We investigated whether images obtained through X-ray micro-computed tomography (μCT) can be used in conjunction with traditional methods for morphological studies of soft-bodied land planarians. μCT is non-invasive and provides true-to-scale three-dimensional imagery at high resolution....
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4793757/ https://www.ncbi.nlm.nih.gov/pubmed/26985364 http://dx.doi.org/10.1186/s13742-016-0119-4 |
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author | Carbayo, Fernando Lenihan, Jennifer Winifred |
author_facet | Carbayo, Fernando Lenihan, Jennifer Winifred |
author_sort | Carbayo, Fernando |
collection | PubMed |
description | BACKGROUND: We investigated whether images obtained through X-ray micro-computed tomography (μCT) can be used in conjunction with traditional methods for morphological studies of soft-bodied land planarians. μCT is non-invasive and provides true-to-scale three-dimensional imagery at high resolution. We compared μCT-based images of a recently described land planarian species of Obama otavioi (Platyhelminthes) with those obtained from light microphotography of histological sections, most of which were also digitized at high magnification. FINDINGS: The specimens studied were collected in 2012. Subsequent μCT-based images of the stained body of a paratype show nearly all morphological features provided by traditional histology, with the exception of particularly minute structures, smaller than 5 μm, such as the sensory pits and single muscle fibers, which are best visible on traditional histological sections. Because the technique is non-destructive, the scanned specimen is preserved without damage. The raw and derivative μCT data and virtual histological sections are freely available in GigaDB. CONCLUSIONS: The μCT datasets of these stained soft-bodied organisms reveal images of external and internal structures that support previous taxonomic studies. This technique can be particularly important for non-destructively revealing internal details of whole museum specimens at a faster rate than histology alone. High-resolution virtual histological slides also allow further searches for new, previously unstudied morphological features. The use of X-ray equipment with higher resolution can enable smaller sensory organ and muscle fiber details to be seen. The image sets, μCT-based images and digitized histological slides can be disseminated without the constraints of specimen loans. |
format | Online Article Text |
id | pubmed-4793757 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-47937572016-03-17 Micro-computed tomography scan and virtual histological slide data for the land planarian Obama otavioi (Platyhelminthes) Carbayo, Fernando Lenihan, Jennifer Winifred Gigascience Data Note BACKGROUND: We investigated whether images obtained through X-ray micro-computed tomography (μCT) can be used in conjunction with traditional methods for morphological studies of soft-bodied land planarians. μCT is non-invasive and provides true-to-scale three-dimensional imagery at high resolution. We compared μCT-based images of a recently described land planarian species of Obama otavioi (Platyhelminthes) with those obtained from light microphotography of histological sections, most of which were also digitized at high magnification. FINDINGS: The specimens studied were collected in 2012. Subsequent μCT-based images of the stained body of a paratype show nearly all morphological features provided by traditional histology, with the exception of particularly minute structures, smaller than 5 μm, such as the sensory pits and single muscle fibers, which are best visible on traditional histological sections. Because the technique is non-destructive, the scanned specimen is preserved without damage. The raw and derivative μCT data and virtual histological sections are freely available in GigaDB. CONCLUSIONS: The μCT datasets of these stained soft-bodied organisms reveal images of external and internal structures that support previous taxonomic studies. This technique can be particularly important for non-destructively revealing internal details of whole museum specimens at a faster rate than histology alone. High-resolution virtual histological slides also allow further searches for new, previously unstudied morphological features. The use of X-ray equipment with higher resolution can enable smaller sensory organ and muscle fiber details to be seen. The image sets, μCT-based images and digitized histological slides can be disseminated without the constraints of specimen loans. BioMed Central 2016-03-16 /pmc/articles/PMC4793757/ /pubmed/26985364 http://dx.doi.org/10.1186/s13742-016-0119-4 Text en © Carbayo and Lenihan. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Data Note Carbayo, Fernando Lenihan, Jennifer Winifred Micro-computed tomography scan and virtual histological slide data for the land planarian Obama otavioi (Platyhelminthes) |
title | Micro-computed tomography scan and virtual histological slide data for the land planarian Obama otavioi (Platyhelminthes) |
title_full | Micro-computed tomography scan and virtual histological slide data for the land planarian Obama otavioi (Platyhelminthes) |
title_fullStr | Micro-computed tomography scan and virtual histological slide data for the land planarian Obama otavioi (Platyhelminthes) |
title_full_unstemmed | Micro-computed tomography scan and virtual histological slide data for the land planarian Obama otavioi (Platyhelminthes) |
title_short | Micro-computed tomography scan and virtual histological slide data for the land planarian Obama otavioi (Platyhelminthes) |
title_sort | micro-computed tomography scan and virtual histological slide data for the land planarian obama otavioi (platyhelminthes) |
topic | Data Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4793757/ https://www.ncbi.nlm.nih.gov/pubmed/26985364 http://dx.doi.org/10.1186/s13742-016-0119-4 |
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