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Transcriptome Analysis for Abnormal Spike Development of the Wheat Mutant dms

BACKGROUND: Wheat (Triticum aestivum L.) spike development is the foundation for grain yield. We obtained a novel wheat mutant, dms, characterized as dwarf, multi-pistil and sterility. Although the genetic changes are not clear, the heredity of traits suggests that a recessive gene locus controls th...

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Autores principales: Zhu, Xin-Xin, Li, Qiao-Yun, Shen, Chun-Cai, Duan, Zong-Biao, Yu, Dong-Yan, Niu, Ji-Shan, Ni, Yong-Jing, Jiang, Yu-Mei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4794226/
https://www.ncbi.nlm.nih.gov/pubmed/26982202
http://dx.doi.org/10.1371/journal.pone.0149287
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author Zhu, Xin-Xin
Li, Qiao-Yun
Shen, Chun-Cai
Duan, Zong-Biao
Yu, Dong-Yan
Niu, Ji-Shan
Ni, Yong-Jing
Jiang, Yu-Mei
author_facet Zhu, Xin-Xin
Li, Qiao-Yun
Shen, Chun-Cai
Duan, Zong-Biao
Yu, Dong-Yan
Niu, Ji-Shan
Ni, Yong-Jing
Jiang, Yu-Mei
author_sort Zhu, Xin-Xin
collection PubMed
description BACKGROUND: Wheat (Triticum aestivum L.) spike development is the foundation for grain yield. We obtained a novel wheat mutant, dms, characterized as dwarf, multi-pistil and sterility. Although the genetic changes are not clear, the heredity of traits suggests that a recessive gene locus controls the two traits of multi-pistil and sterility in self-pollinating populations of the medium plants (M), such that the dwarf genotype (D) and tall genotype (T) in the progeny of the mutant are ideal lines for studies regarding wheat spike development. The objective of this study was to explore the molecular basis for spike abnormalities of dwarf genotype. RESULTS: Four unigene libraries were assembled by sequencing the mRNAs of the super-bulked differentiating spikes and stem tips of the D and T plants. Using integrative analysis, we identified 419 genes highly expressed in spikes, including nine typical homeotic genes of the MADS-box family and the genes TaAP2, TaFL and TaDL. We also identified 143 genes that were significantly different between young spikes of T and D, and 26 genes that were putatively involved in spike differentiation. The result showed that the expression levels of TaAP1-2, TaAP2, and other genes involved in the majority of biological processes such as transcription, translation, cell division, photosynthesis, carbohydrate transport and metabolism, and energy production and conversion were significantly lower in D than in T. CONCLUSIONS: We identified a set of genes related to wheat floral organ differentiation, including typical homeotic genes. Our results showed that the major causal factors resulting in the spike abnormalities of dms were the lower expression homeotic genes, hormonal imbalance, repressed biological processes, and deficiency of construction materials and energy. We performed a series of studies on the homeotic genes, however the other three causal factors for spike abnormal phenotype of dms need further study.
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spelling pubmed-47942262016-03-23 Transcriptome Analysis for Abnormal Spike Development of the Wheat Mutant dms Zhu, Xin-Xin Li, Qiao-Yun Shen, Chun-Cai Duan, Zong-Biao Yu, Dong-Yan Niu, Ji-Shan Ni, Yong-Jing Jiang, Yu-Mei PLoS One Research Article BACKGROUND: Wheat (Triticum aestivum L.) spike development is the foundation for grain yield. We obtained a novel wheat mutant, dms, characterized as dwarf, multi-pistil and sterility. Although the genetic changes are not clear, the heredity of traits suggests that a recessive gene locus controls the two traits of multi-pistil and sterility in self-pollinating populations of the medium plants (M), such that the dwarf genotype (D) and tall genotype (T) in the progeny of the mutant are ideal lines for studies regarding wheat spike development. The objective of this study was to explore the molecular basis for spike abnormalities of dwarf genotype. RESULTS: Four unigene libraries were assembled by sequencing the mRNAs of the super-bulked differentiating spikes and stem tips of the D and T plants. Using integrative analysis, we identified 419 genes highly expressed in spikes, including nine typical homeotic genes of the MADS-box family and the genes TaAP2, TaFL and TaDL. We also identified 143 genes that were significantly different between young spikes of T and D, and 26 genes that were putatively involved in spike differentiation. The result showed that the expression levels of TaAP1-2, TaAP2, and other genes involved in the majority of biological processes such as transcription, translation, cell division, photosynthesis, carbohydrate transport and metabolism, and energy production and conversion were significantly lower in D than in T. CONCLUSIONS: We identified a set of genes related to wheat floral organ differentiation, including typical homeotic genes. Our results showed that the major causal factors resulting in the spike abnormalities of dms were the lower expression homeotic genes, hormonal imbalance, repressed biological processes, and deficiency of construction materials and energy. We performed a series of studies on the homeotic genes, however the other three causal factors for spike abnormal phenotype of dms need further study. Public Library of Science 2016-03-16 /pmc/articles/PMC4794226/ /pubmed/26982202 http://dx.doi.org/10.1371/journal.pone.0149287 Text en © 2016 Zhu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhu, Xin-Xin
Li, Qiao-Yun
Shen, Chun-Cai
Duan, Zong-Biao
Yu, Dong-Yan
Niu, Ji-Shan
Ni, Yong-Jing
Jiang, Yu-Mei
Transcriptome Analysis for Abnormal Spike Development of the Wheat Mutant dms
title Transcriptome Analysis for Abnormal Spike Development of the Wheat Mutant dms
title_full Transcriptome Analysis for Abnormal Spike Development of the Wheat Mutant dms
title_fullStr Transcriptome Analysis for Abnormal Spike Development of the Wheat Mutant dms
title_full_unstemmed Transcriptome Analysis for Abnormal Spike Development of the Wheat Mutant dms
title_short Transcriptome Analysis for Abnormal Spike Development of the Wheat Mutant dms
title_sort transcriptome analysis for abnormal spike development of the wheat mutant dms
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4794226/
https://www.ncbi.nlm.nih.gov/pubmed/26982202
http://dx.doi.org/10.1371/journal.pone.0149287
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