Double‐strand break repair based on short‐homology regions is suppressed under terminal deoxynucleotidyltransferase expression, as revealed by a novel vector system for analysing DNA repair by nonhomologous end joining

We have constructed a novel, nonhomologous end‐joining (NHEJ) assay vector (NAV), containing mKate2, Venus and ccdB genes. Cotransfection of NAV with a construct expressing the restriction enzyme I‐SceI generated a double‐strand break (DSB) in NAV that excised mKate2 and ccdB. Repair of this DSB pro...

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Detalles Bibliográficos
Autores principales: Maezawa, So, Nakano, Saori, Kuniya, Takaaki, Koiwai, Osamu, Koiwai, Kotaro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4794791/
https://www.ncbi.nlm.nih.gov/pubmed/27047738
http://dx.doi.org/10.1002/2211-5463.12001

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4794791/
https://www.ncbi.nlm.nih.gov/pubmed/27047738
http://dx.doi.org/10.1002/2211-5463.12001

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