Germ cell‐specific expression of Cre recombinase using the VASA promoter in the pig

The Cre–loxP system is a powerful tool for genetic analysis of distinct cell lineages and tissue‐specific gene knockout in animal models. VASA is specifically expressed in reproductive tissues, and is known to play important roles in spermatogenesis and germ‐cell growth. In this study, Cre recombina...

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Detalles Bibliográficos
Autores principales: Song, Yuning, Lai, Liangxue, Li, Li, Huang, Yongye, Wang, Anfeng, Tang, Xiaochun, Pang, Daxin, Li, Zhanjun, Ouyang, Hongsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2015
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4794798/
https://www.ncbi.nlm.nih.gov/pubmed/27047735
http://dx.doi.org/10.1002/2211-5463.12005
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author Song, Yuning
Lai, Liangxue
Li, Li
Huang, Yongye
Wang, Anfeng
Tang, Xiaochun
Pang, Daxin
Li, Zhanjun
Ouyang, Hongsheng
author_facet Song, Yuning
Lai, Liangxue
Li, Li
Huang, Yongye
Wang, Anfeng
Tang, Xiaochun
Pang, Daxin
Li, Zhanjun
Ouyang, Hongsheng
author_sort Song, Yuning
collection PubMed
description The Cre–loxP system is a powerful tool for genetic analysis of distinct cell lineages and tissue‐specific gene knockout in animal models. VASA is specifically expressed in reproductive tissues, and is known to play important roles in spermatogenesis and germ‐cell growth. In this study, Cre recombinase transgenic pigs under the control of the VASA promoter were generated by somatic cell nuclear transfer. Germ cell‐specific expression of Cre recombinase in VASA‐Cre transgenic pigs was shown by western blotting and immunohistochemistry. VASA‐Cre transgenic pigs will be a useful tool for germ cell‐specific gene knockout and a disease model for disorders of the reproductive system.
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spelling pubmed-47947982016-04-04 Germ cell‐specific expression of Cre recombinase using the VASA promoter in the pig Song, Yuning Lai, Liangxue Li, Li Huang, Yongye Wang, Anfeng Tang, Xiaochun Pang, Daxin Li, Zhanjun Ouyang, Hongsheng FEBS Open Bio Research Articles The Cre–loxP system is a powerful tool for genetic analysis of distinct cell lineages and tissue‐specific gene knockout in animal models. VASA is specifically expressed in reproductive tissues, and is known to play important roles in spermatogenesis and germ‐cell growth. In this study, Cre recombinase transgenic pigs under the control of the VASA promoter were generated by somatic cell nuclear transfer. Germ cell‐specific expression of Cre recombinase in VASA‐Cre transgenic pigs was shown by western blotting and immunohistochemistry. VASA‐Cre transgenic pigs will be a useful tool for germ cell‐specific gene knockout and a disease model for disorders of the reproductive system. John Wiley and Sons Inc. 2015-12-29 /pmc/articles/PMC4794798/ /pubmed/27047735 http://dx.doi.org/10.1002/2211-5463.12005 Text en © 2015 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Song, Yuning
Lai, Liangxue
Li, Li
Huang, Yongye
Wang, Anfeng
Tang, Xiaochun
Pang, Daxin
Li, Zhanjun
Ouyang, Hongsheng
Germ cell‐specific expression of Cre recombinase using the VASA promoter in the pig
title Germ cell‐specific expression of Cre recombinase using the VASA promoter in the pig
title_full Germ cell‐specific expression of Cre recombinase using the VASA promoter in the pig
title_fullStr Germ cell‐specific expression of Cre recombinase using the VASA promoter in the pig
title_full_unstemmed Germ cell‐specific expression of Cre recombinase using the VASA promoter in the pig
title_short Germ cell‐specific expression of Cre recombinase using the VASA promoter in the pig
title_sort germ cell‐specific expression of cre recombinase using the vasa promoter in the pig
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4794798/
https://www.ncbi.nlm.nih.gov/pubmed/27047735
http://dx.doi.org/10.1002/2211-5463.12005
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