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Metagenomic Survey of Viral Diversity Obtained from Feces of Subantarctic and South American Fur Seals

The Brazilian South coast seasonally hosts numerous marine species, observed particularly during winter months. Some animals, including fur seals, are found dead or debilitated along the shore and may harbor potential pathogens within their microbiota. In the present study, a metagenomic approach wa...

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Autores principales: Kluge, Mariana, Campos, Fabrício Souza, Tavares, Maurício, de Amorim, Derek Blaese, Valdez, Fernanda Pedone, Giongo, Adriana, Roehe, Paulo Michel, Franco, Ana Claudia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4795697/
https://www.ncbi.nlm.nih.gov/pubmed/26986573
http://dx.doi.org/10.1371/journal.pone.0151921
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author Kluge, Mariana
Campos, Fabrício Souza
Tavares, Maurício
de Amorim, Derek Blaese
Valdez, Fernanda Pedone
Giongo, Adriana
Roehe, Paulo Michel
Franco, Ana Claudia
author_facet Kluge, Mariana
Campos, Fabrício Souza
Tavares, Maurício
de Amorim, Derek Blaese
Valdez, Fernanda Pedone
Giongo, Adriana
Roehe, Paulo Michel
Franco, Ana Claudia
author_sort Kluge, Mariana
collection PubMed
description The Brazilian South coast seasonally hosts numerous marine species, observed particularly during winter months. Some animals, including fur seals, are found dead or debilitated along the shore and may harbor potential pathogens within their microbiota. In the present study, a metagenomic approach was performed to evaluate the viral diversity in feces of fur seals found deceased along the coast of the state of Rio Grande do Sul. The fecal virome of two fur seal species was characterized: the South American fur seal (Arctocephalus australis) and the Subantarctic fur seal (Arctocephalus tropicalis). Fecal samples from 10 specimens (A. australis, n = 5; A. tropicalis, n = 5) were collected and viral particles were purified, extracted and amplified with a random PCR. The products were sequenced through Ion Torrent and Illumina platforms and assembled reads were submitted to BLASTx searches. Both viromes were dominated by bacteriophages and included a number of potentially novel virus genomes. Sequences of picobirnaviruses, picornaviruses and a hepevirus-like were identified in A. australis. A rotavirus related to group C, a novel member of the Sakobuvirus and a sapovirus very similar to California sea lion sapovirus 1 were found in A. tropicalis. Additionally, sequences of members of the Anelloviridae and Parvoviridae families were detected in both fur seal species. This is the first metagenomic study to screen the fecal virome of fur seals, contributing to a better understanding of the complexity of the viral community present in the intestinal microbiota of these animals.
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spelling pubmed-47956972016-03-23 Metagenomic Survey of Viral Diversity Obtained from Feces of Subantarctic and South American Fur Seals Kluge, Mariana Campos, Fabrício Souza Tavares, Maurício de Amorim, Derek Blaese Valdez, Fernanda Pedone Giongo, Adriana Roehe, Paulo Michel Franco, Ana Claudia PLoS One Research Article The Brazilian South coast seasonally hosts numerous marine species, observed particularly during winter months. Some animals, including fur seals, are found dead or debilitated along the shore and may harbor potential pathogens within their microbiota. In the present study, a metagenomic approach was performed to evaluate the viral diversity in feces of fur seals found deceased along the coast of the state of Rio Grande do Sul. The fecal virome of two fur seal species was characterized: the South American fur seal (Arctocephalus australis) and the Subantarctic fur seal (Arctocephalus tropicalis). Fecal samples from 10 specimens (A. australis, n = 5; A. tropicalis, n = 5) were collected and viral particles were purified, extracted and amplified with a random PCR. The products were sequenced through Ion Torrent and Illumina platforms and assembled reads were submitted to BLASTx searches. Both viromes were dominated by bacteriophages and included a number of potentially novel virus genomes. Sequences of picobirnaviruses, picornaviruses and a hepevirus-like were identified in A. australis. A rotavirus related to group C, a novel member of the Sakobuvirus and a sapovirus very similar to California sea lion sapovirus 1 were found in A. tropicalis. Additionally, sequences of members of the Anelloviridae and Parvoviridae families were detected in both fur seal species. This is the first metagenomic study to screen the fecal virome of fur seals, contributing to a better understanding of the complexity of the viral community present in the intestinal microbiota of these animals. Public Library of Science 2016-03-17 /pmc/articles/PMC4795697/ /pubmed/26986573 http://dx.doi.org/10.1371/journal.pone.0151921 Text en © 2016 Kluge et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kluge, Mariana
Campos, Fabrício Souza
Tavares, Maurício
de Amorim, Derek Blaese
Valdez, Fernanda Pedone
Giongo, Adriana
Roehe, Paulo Michel
Franco, Ana Claudia
Metagenomic Survey of Viral Diversity Obtained from Feces of Subantarctic and South American Fur Seals
title Metagenomic Survey of Viral Diversity Obtained from Feces of Subantarctic and South American Fur Seals
title_full Metagenomic Survey of Viral Diversity Obtained from Feces of Subantarctic and South American Fur Seals
title_fullStr Metagenomic Survey of Viral Diversity Obtained from Feces of Subantarctic and South American Fur Seals
title_full_unstemmed Metagenomic Survey of Viral Diversity Obtained from Feces of Subantarctic and South American Fur Seals
title_short Metagenomic Survey of Viral Diversity Obtained from Feces of Subantarctic and South American Fur Seals
title_sort metagenomic survey of viral diversity obtained from feces of subantarctic and south american fur seals
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4795697/
https://www.ncbi.nlm.nih.gov/pubmed/26986573
http://dx.doi.org/10.1371/journal.pone.0151921
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