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A Next-Generation Sequencing Data Analysis Pipeline for Detecting Unknown Pathogens from Mixed Clinical Samples and Revealing Their Genetic Diversity
Forty-two cytopathic effect (CPE)-positive isolates were collected from 2008 to 2012. All isolates could not be identified for known viral pathogens by routine diagnostic assays. They were pooled into 8 groups of 5–6 isolates to reduce the sequencing cost. Next-generation sequencing (NGS) was conduc...
Autores principales: | Gong, Yu-Nong, Chen, Guang-Wu, Yang, Shu-Li, Lee, Ching-Ju, Shih, Shin-Ru, Tsao, Kuo-Chien |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4795770/ https://www.ncbi.nlm.nih.gov/pubmed/26986479 http://dx.doi.org/10.1371/journal.pone.0151495 |
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