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A Next-Generation Sequencing Data Analysis Pipeline for Detecting Unknown Pathogens from Mixed Clinical Samples and Revealing Their Genetic Diversity

Forty-two cytopathic effect (CPE)-positive isolates were collected from 2008 to 2012. All isolates could not be identified for known viral pathogens by routine diagnostic assays. They were pooled into 8 groups of 5–6 isolates to reduce the sequencing cost. Next-generation sequencing (NGS) was conduc...

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Detalles Bibliográficos
Autores principales: Gong, Yu-Nong, Chen, Guang-Wu, Yang, Shu-Li, Lee, Ching-Ju, Shih, Shin-Ru, Tsao, Kuo-Chien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4795770/
https://www.ncbi.nlm.nih.gov/pubmed/26986479
http://dx.doi.org/10.1371/journal.pone.0151495

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