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An ultra-dense library resource for rapid deconvolution of mutations that cause phenotypes in Escherichia coli

With the wide availability of whole-genome sequencing (WGS), genetic mapping has become the rate-limiting step, inhibiting unbiased forward genetics in even the most tractable model organisms. We introduce a rapid deconvolution resource and method for untagged causative mutations after mutagenesis,...

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Detalles Bibliográficos
Autores principales:	Nehring, Ralf B., Gu, Franklin, Lin, Hsin-Yu, Gibson, Janet L., Blythe, Martin J., Wilson, Ray, Bravo Núñez, María Angélica, Hastings, P. J., Louis, Edward J., Frisch, Ryan L., Hu, James C., Rosenberg, Susan M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2016
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4797258/ https://www.ncbi.nlm.nih.gov/pubmed/26578563 http://dx.doi.org/10.1093/nar/gkv1131

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