Cargando…

An ultra-dense library resource for rapid deconvolution of mutations that cause phenotypes in Escherichia coli

With the wide availability of whole-genome sequencing (WGS), genetic mapping has become the rate-limiting step, inhibiting unbiased forward genetics in even the most tractable model organisms. We introduce a rapid deconvolution resource and method for untagged causative mutations after mutagenesis,...

Descripción completa

Detalles Bibliográficos
Autores principales:	Nehring, Ralf B., Gu, Franklin, Lin, Hsin-Yu, Gibson, Janet L., Blythe, Martin J., Wilson, Ray, Bravo Núñez, María Angélica, Hastings, P. J., Louis, Edward J., Frisch, Ryan L., Hu, James C., Rosenberg, Susan M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2016
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4797258/ https://www.ncbi.nlm.nih.gov/pubmed/26578563 http://dx.doi.org/10.1093/nar/gkv1131

Ejemplares similares

Deconvolution of nucleic-acid length distributions: a gel electrophoresis analysis tool and applications
por: Ziraldo, Riccardo, et al.
Publicado: (2019)

SPOTlight: seeded NMF regression to deconvolute spatial transcriptomics spots with single-cell transcriptomes
por: Elosua-Bayes, Marc, et al.
Publicado: (2021)

DeCompress: tissue compartment deconvolution of targeted mRNA expression panels using compressed sensing
por: Bhattacharya, Arjun, et al.
Publicado: (2021)

An iterative network partition algorithm for accurate identification of dense network modules
por: Sun, Siqi, et al.
Publicado: (2012)

An optimized split-ubiquitin cDNA-library screening system to identify novel interactors of the human Frizzled 1 receptor
por: Dirnberger, Dietmar, et al.
Publicado: (2008)

The use of multiple displacement amplification to amplify complex DNA libraries
por: Fullwood, Melissa J., et al.
Publicado: (2008)

A transposase strategy for creating libraries of circularly permuted proteins
por: Mehta, Manan M., et al.
Publicado: (2012)

Extensive libraries of gene truncation variants generated by in vitro transposition
por: Morelli, Aleardo, et al.
Publicado: (2017)

Selection of functional human antibodies from retroviral display libraries
por: Urban, Johannes H., et al.
Publicado: (2005)

A platform for functional assessment of large variant libraries in mammalian cells
por: Matreyek, Kenneth A., et al.
Publicado: (2017)

Impact of next-generation sequencing error on analysis of barcoded plasmid libraries of known complexity and sequence
por: Deakin, Claire T., et al.
Publicado: (2014)

An improved platform for functional assessment of large protein libraries in mammalian cells
por: Matreyek, Kenneth A, et al.
Publicado: (2020)

Circular permutation profiling by deep sequencing libraries created using transposon mutagenesis
por: Atkinson, Joshua T, et al.
Publicado: (2018)

Covalent antibody display—an in vitro antibody-DNA library selection system
por: Reiersen, Herald, et al.
Publicado: (2005)

Screening of retroviral cDNA libraries for factors involved in protein phosphorylation in signaling cascades
por: Stitz, J., et al.
Publicado: (2005)

Highly cost-efficient genome-wide association studies using DNA pools and dense SNP arrays
por: Macgregor, Stuart, et al.
Publicado: (2008)

Targeting individual cells by barcode in pooled sequence libraries
por: Ranu, Navpreet, et al.
Publicado: (2019)

A novel NGS library preparation method to characterize native termini of fragmented DNA
por: Harkins, Kelly M, et al.
Publicado: (2020)

A low-bias and sensitive small RNA library preparation method using randomized splint ligation
por: Maguire, Sean, et al.
Publicado: (2020)

A genome-wide library of CB4856/N2 introgression lines of Caenorhabditis elegans
por: Doroszuk, Agnieszka, et al.
Publicado: (2009)

A simple and rapid method for enzymatic synthesis of CRISPR-Cas9 sgRNA libraries
por: Yates, Joshua D, et al.
Publicado: (2021)

Development and application of a recombination-based library versus library high- throughput yeast two-hybrid (RLL-Y2H) screening system
por: Yang, Fang, et al.
Publicado: (2018)

A multipurpose vector system for the screening of libraries in bacteria, insect and mammalian cells and expression in vivo
por: Laitinen, Olli H., et al.
Publicado: (2005)

CLUE: a bioinformatic and wet-lab pipeline for multiplexed cloning of custom sgRNA libraries
por: Becker, Martin, et al.
Publicado: (2020)

Integrase-directed recovery of functional genes from genomic libraries
por: Rowe-Magnus, Dean A.
Publicado: (2009)

Phage display peptide libraries: deviations from randomness and correctives
por: Ryvkin, Arie, et al.
Publicado: (2018)

Flexible CRISPR library construction using parallel oligonucleotide retrieval
por: Read, Abigail, et al.
Publicado: (2017)

Targeted insertional mutagenesis libraries for deep domain insertion profiling
por: Coyote-Maestas, Willow, et al.
Publicado: (2020)

Assessing serotonin receptor mRNA editing frequency by a novel ultra high-throughput sequencing method
por: Abbas, Atheir I., et al.
Publicado: (2010)

CRISPR/Cas9-targeted removal of unwanted sequences from small-RNA sequencing libraries
por: Hardigan, Andrew A, et al.
Publicado: (2019)

Accurate SNP and mutation detection by targeted custom microarray-based genomic enrichment of short-fragment sequencing libraries
por: Mokry, Michal, et al.
Publicado: (2010)

An optimized kit-free method for making strand-specific deep sequencing libraries from RNA fragments
por: Heyer, Erin E., et al.
Publicado: (2015)

RNPomics: Defining the ncRNA transcriptome by cDNA library generation from ribonucleo-protein particles
por: Rederstorff, Mathieu, et al.
Publicado: (2010)

Promoter library designed for fine-tuned gene expression in Pichia pastoris
por: Hartner, Franz S., et al.
Publicado: (2008)

An on-bead tailing/ligation approach for sequencing resin-bound RNA libraries
por: Wiesmayr, Anna, et al.
Publicado: (2012)

Synthesis and cell-free cloning of DNA libraries using programmable microfluidics
por: Yehezkel, Tuval Ben, et al.
Publicado: (2016)

Blocking of targeted microRNAs from next-generation sequencing libraries
por: Roberts, Brian S., et al.
Publicado: (2015)

Microfluidic automated plasmid library enrichment for biosynthetic gene cluster discovery
por: Xu, Peng, et al.
Publicado: (2020)

One step construction of PCR mutagenized libraries for genetic analysis by recombination cloning
por: Khalil, Al-Muataz, et al.
Publicado: (2007)

A novel method for generating and screening peptides and libraries displayed on adenovirus fiber
por: Lupold, Shawn E., et al.
Publicado: (2007)

Cannot write session to /tmp/vufind_sessions/sess_t5d6vriohqlebpeou3j8bidbpa