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Kinetic mechanism and fidelity of nick sealing by Escherichia coli NAD(+)-dependent DNA ligase (LigA)
Escherichia coli DNA ligase (EcoLigA) repairs 3′-OH/5′-PO(4) nicks in duplex DNA via reaction of LigA with NAD(+) to form a covalent LigA-(lysyl-Nζ)–AMP intermediate (step 1); transfer of AMP to the nick 5′-PO(4) to form an AppDNA intermediate (step 2); and attack of the nick 3′-OH on AppDNA to form...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Oxford University Press
2016
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4797296/ https://www.ncbi.nlm.nih.gov/pubmed/26857547 http://dx.doi.org/10.1093/nar/gkw049 |