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Lutzomyia migonei is a permissive vector competent for Leishmania infantum
BACKGROUND: Leishmania infantum is the most widespread etiological agent of visceral leishmaniasis (VL) in the world, with significant mortality rates in human cases. In Latin America, this parasite is primarily transmitted by Lutzomyia longipalpis, but the role of Lutzomyia migonei as a potential v...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4797322/ https://www.ncbi.nlm.nih.gov/pubmed/26988559 http://dx.doi.org/10.1186/s13071-016-1444-2 |
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author | Guimarães, Vanessa Cristina Fitipaldi Veloso Pruzinova, Katerina Sadlova, Jovana Volfova, Vera Myskova, Jitka Filho, Sinval Pinto Brandão Volf, Petr |
author_facet | Guimarães, Vanessa Cristina Fitipaldi Veloso Pruzinova, Katerina Sadlova, Jovana Volfova, Vera Myskova, Jitka Filho, Sinval Pinto Brandão Volf, Petr |
author_sort | Guimarães, Vanessa Cristina Fitipaldi Veloso |
collection | PubMed |
description | BACKGROUND: Leishmania infantum is the most widespread etiological agent of visceral leishmaniasis (VL) in the world, with significant mortality rates in human cases. In Latin America, this parasite is primarily transmitted by Lutzomyia longipalpis, but the role of Lutzomyia migonei as a potential vector for this protozoan has been discussed. Laboratory and field investigations have contributed to this hypothesis; however, proof of the vector competence of L. migonei has not yet been provided. In this study, we evaluate for the first time the susceptibility of L. migonei to L. infantum. METHODS: Females of laboratory-reared L. migonei were fed through a chick-skin membrane on rabbit blood containing L. infantum promastigotes, dissected at 1, 5 and 8 days post-infection (PI) and checked microscopically for the presence, intensity and localisation of Leishmania infections. In addition, morphometric analysis of L. infantum promastigotes was performed. RESULTS: High infection rates of both L. infantum strains tested were observed in L. migonei, with colonisation of the stomodeal valve already on day 5 PI. At the late-stage infection, most L. migonei females had their cardia and stomodeal valve colonised by high numbers of parasites, and no significant differences were found compared to the development in L. longipalpis. Metacyclic forms were found in all parasite-vector combinations since day 5 PI. CONCLUSIONS: We propose that Lutzomyia migonei belongs to sand fly species permissive to various Leishmania spp. Here we demonstrate that L. migonei is highly susceptible to the development of L. infantum. This, together with its known anthropophily, abundance in VL foci and natural infection by L. infantum, constitute important evidence that L. migonei is another vector of this parasite in Latin America. |
format | Online Article Text |
id | pubmed-4797322 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-47973222016-03-19 Lutzomyia migonei is a permissive vector competent for Leishmania infantum Guimarães, Vanessa Cristina Fitipaldi Veloso Pruzinova, Katerina Sadlova, Jovana Volfova, Vera Myskova, Jitka Filho, Sinval Pinto Brandão Volf, Petr Parasit Vectors Research BACKGROUND: Leishmania infantum is the most widespread etiological agent of visceral leishmaniasis (VL) in the world, with significant mortality rates in human cases. In Latin America, this parasite is primarily transmitted by Lutzomyia longipalpis, but the role of Lutzomyia migonei as a potential vector for this protozoan has been discussed. Laboratory and field investigations have contributed to this hypothesis; however, proof of the vector competence of L. migonei has not yet been provided. In this study, we evaluate for the first time the susceptibility of L. migonei to L. infantum. METHODS: Females of laboratory-reared L. migonei were fed through a chick-skin membrane on rabbit blood containing L. infantum promastigotes, dissected at 1, 5 and 8 days post-infection (PI) and checked microscopically for the presence, intensity and localisation of Leishmania infections. In addition, morphometric analysis of L. infantum promastigotes was performed. RESULTS: High infection rates of both L. infantum strains tested were observed in L. migonei, with colonisation of the stomodeal valve already on day 5 PI. At the late-stage infection, most L. migonei females had their cardia and stomodeal valve colonised by high numbers of parasites, and no significant differences were found compared to the development in L. longipalpis. Metacyclic forms were found in all parasite-vector combinations since day 5 PI. CONCLUSIONS: We propose that Lutzomyia migonei belongs to sand fly species permissive to various Leishmania spp. Here we demonstrate that L. migonei is highly susceptible to the development of L. infantum. This, together with its known anthropophily, abundance in VL foci and natural infection by L. infantum, constitute important evidence that L. migonei is another vector of this parasite in Latin America. BioMed Central 2016-03-17 /pmc/articles/PMC4797322/ /pubmed/26988559 http://dx.doi.org/10.1186/s13071-016-1444-2 Text en © Guimarães et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Guimarães, Vanessa Cristina Fitipaldi Veloso Pruzinova, Katerina Sadlova, Jovana Volfova, Vera Myskova, Jitka Filho, Sinval Pinto Brandão Volf, Petr Lutzomyia migonei is a permissive vector competent for Leishmania infantum |
title | Lutzomyia migonei is a permissive vector competent for Leishmania infantum |
title_full | Lutzomyia migonei is a permissive vector competent for Leishmania infantum |
title_fullStr | Lutzomyia migonei is a permissive vector competent for Leishmania infantum |
title_full_unstemmed | Lutzomyia migonei is a permissive vector competent for Leishmania infantum |
title_short | Lutzomyia migonei is a permissive vector competent for Leishmania infantum |
title_sort | lutzomyia migonei is a permissive vector competent for leishmania infantum |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4797322/ https://www.ncbi.nlm.nih.gov/pubmed/26988559 http://dx.doi.org/10.1186/s13071-016-1444-2 |
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