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Effect of Temperature Downshift on the Transcriptomic Responses of Chinese Hamster Ovary Cells Using Recombinant Human Tissue Plasminogen Activator Production Culture

Recombinant proteins are widely used as biopharmaceuticals, but their production by mammalian cell culture is expensive. Hence, improvement of bioprocess productivity is greatly needed. A temperature downshift (TDS) from 37°C to 28–34°C is an effective strategy to expand the productive life period o...

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Detalles Bibliográficos
Autores principales:	Bedoya-López, Andrea, Estrada, Karel, Sanchez-Flores, Alejandro, Ramírez, Octavio T., Altamirano, Claudia, Segovia, Lorenzo, Miranda-Ríos, Juan, Trujillo-Roldán, Mauricio A., Valdez-Cruz, Norma A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2016
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4798216/ https://www.ncbi.nlm.nih.gov/pubmed/26991106 http://dx.doi.org/10.1371/journal.pone.0151529

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