Cargando…
DNA isolation from teeth by organic extraction and identification of sex of the individual by analyzing the AMEL gene marker using PCR
BACKGROUND: To identify the sex of the deceased individual from dental hard tissue such as enamel and dentine. OBJECTIVE: To isolate the DNA from dental hard tissue (enamel and dentin) from teeth extracted for prophylactic purpose, to assess the quality and purity of DNA and to identify the sex usin...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4799513/ https://www.ncbi.nlm.nih.gov/pubmed/27051218 http://dx.doi.org/10.4103/0975-1475.176953 |
_version_ | 1782422361195151360 |
---|---|
author | Praveen Kumar, Subramanian Thangaraj Aswath, Nalini |
author_facet | Praveen Kumar, Subramanian Thangaraj Aswath, Nalini |
author_sort | Praveen Kumar, Subramanian Thangaraj |
collection | PubMed |
description | BACKGROUND: To identify the sex of the deceased individual from dental hard tissue such as enamel and dentine. OBJECTIVE: To isolate the DNA from dental hard tissue (enamel and dentin) from teeth extracted for prophylactic purpose, to assess the quality and purity of DNA and to identify the sex using polymerized chain reactor (PCR). MATERIALS AND METHODS: DNA was extracted following phenol/chloroform (organic) extraction from 20 male and 20 female teeth. The samples that contain the amelogenin gene (amel) were amplified by PCR. The products of the PCR were run on agarose gel with ethidium bromide staining on gel documentation system. RESULTS: The results on the gel showed the presence of X-specific bands at 212 bp and Y-specific bands at 218 bp. Males were distinguished from females by the presence of two bands whereas female samples showed only one, that is, X-specific band on the gel. The gender from the known samples was determined with complete accuracy, and the results were analyzed statistically by the Chi-square test. CONCLUSION: In our study, the PCR-based method showed 100% specificity and sensitivity. |
format | Online Article Text |
id | pubmed-4799513 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-47995132016-04-05 DNA isolation from teeth by organic extraction and identification of sex of the individual by analyzing the AMEL gene marker using PCR Praveen Kumar, Subramanian Thangaraj Aswath, Nalini J Forensic Dent Sci Original Article BACKGROUND: To identify the sex of the deceased individual from dental hard tissue such as enamel and dentine. OBJECTIVE: To isolate the DNA from dental hard tissue (enamel and dentin) from teeth extracted for prophylactic purpose, to assess the quality and purity of DNA and to identify the sex using polymerized chain reactor (PCR). MATERIALS AND METHODS: DNA was extracted following phenol/chloroform (organic) extraction from 20 male and 20 female teeth. The samples that contain the amelogenin gene (amel) were amplified by PCR. The products of the PCR were run on agarose gel with ethidium bromide staining on gel documentation system. RESULTS: The results on the gel showed the presence of X-specific bands at 212 bp and Y-specific bands at 218 bp. Males were distinguished from females by the presence of two bands whereas female samples showed only one, that is, X-specific band on the gel. The gender from the known samples was determined with complete accuracy, and the results were analyzed statistically by the Chi-square test. CONCLUSION: In our study, the PCR-based method showed 100% specificity and sensitivity. Medknow Publications & Media Pvt Ltd 2016 /pmc/articles/PMC4799513/ /pubmed/27051218 http://dx.doi.org/10.4103/0975-1475.176953 Text en Copyright: © Journal of Forensic Dental Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Praveen Kumar, Subramanian Thangaraj Aswath, Nalini DNA isolation from teeth by organic extraction and identification of sex of the individual by analyzing the AMEL gene marker using PCR |
title | DNA isolation from teeth by organic extraction and identification of sex of the individual by analyzing the AMEL gene marker using PCR |
title_full | DNA isolation from teeth by organic extraction and identification of sex of the individual by analyzing the AMEL gene marker using PCR |
title_fullStr | DNA isolation from teeth by organic extraction and identification of sex of the individual by analyzing the AMEL gene marker using PCR |
title_full_unstemmed | DNA isolation from teeth by organic extraction and identification of sex of the individual by analyzing the AMEL gene marker using PCR |
title_short | DNA isolation from teeth by organic extraction and identification of sex of the individual by analyzing the AMEL gene marker using PCR |
title_sort | dna isolation from teeth by organic extraction and identification of sex of the individual by analyzing the amel gene marker using pcr |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4799513/ https://www.ncbi.nlm.nih.gov/pubmed/27051218 http://dx.doi.org/10.4103/0975-1475.176953 |
work_keys_str_mv | AT praveenkumarsubramanianthangaraj dnaisolationfromteethbyorganicextractionandidentificationofsexoftheindividualbyanalyzingtheamelgenemarkerusingpcr AT aswathnalini dnaisolationfromteethbyorganicextractionandidentificationofsexoftheindividualbyanalyzingtheamelgenemarkerusingpcr |