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Quantification of a peptide standard using the intrinsic fluorescence of tyrosine

Absolute quantification of peptides is typically achieved using amino acid analysis, elemental analysis or derivatisation chemistry. Impurities, if present, may be accounted for using analytical high-performance liquid chromatography (HPLC) with detection of the peptide bond ultraviolet (UV) absorba...

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Detalles Bibliográficos
Autores principales:	Preston, George W., Phillips, David H.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Berlin Heidelberg 2016
Materias:	Rapid Communication
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4799795/ https://www.ncbi.nlm.nih.gov/pubmed/26879647 http://dx.doi.org/10.1007/s00216-016-9334-1

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