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Brief Communication: Maternal Plasma Autoantibodies Screening in Fetal Down Syndrome

Imbalance in the metabolites levels which can potentially be related to certain fetal chromosomal abnormalities can stimulate mother's immune response to produce autoantibodies directed against proteins. The aim of the study was to determine the concentration of 9000 autoantibodies in maternal...

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Autores principales: Charkiewicz, Karol, Zbucka-Kretowska, Monika, Goscik, Joanna, Wolczynski, Slawomir, Lemancewicz, Adam, Laudanski, Piotr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4799815/
https://www.ncbi.nlm.nih.gov/pubmed/27042674
http://dx.doi.org/10.1155/2016/9362169
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author Charkiewicz, Karol
Zbucka-Kretowska, Monika
Goscik, Joanna
Wolczynski, Slawomir
Lemancewicz, Adam
Laudanski, Piotr
author_facet Charkiewicz, Karol
Zbucka-Kretowska, Monika
Goscik, Joanna
Wolczynski, Slawomir
Lemancewicz, Adam
Laudanski, Piotr
author_sort Charkiewicz, Karol
collection PubMed
description Imbalance in the metabolites levels which can potentially be related to certain fetal chromosomal abnormalities can stimulate mother's immune response to produce autoantibodies directed against proteins. The aim of the study was to determine the concentration of 9000 autoantibodies in maternal plasma to detect fetal Down syndrome. Method. We performed 190 amniocenteses and found 10 patients with confirmed fetal Down syndrome (15th–18th weeks of gestation). For the purpose of our control we chose 11 women without confirmed chromosomal aberration. To assess the expression of autoantibodies in the blood plasma, we used a protein microarray, which allows for simultaneous determination of 9000 proteins per sample. Results. We revealed 213 statistically significant autoantibodies, whose expression decreased or increased in the study group with fetal Down syndrome. The second step was to create a classifier of Down syndrome pregnancy, which includes 14 antibodies. The predictive value of the classifier (specificity and sensitivity) is 100%, classification errors, 0%, cross-validation errors, 0%. Conclusion. Our findings suggest that the autoantibodies may play a role in the pathophysiology of Down syndrome pregnancy. Defining their potential as biochemical markers of Down syndrome pregnancy requires further investigation on larger group of patients.
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spelling pubmed-47998152016-04-03 Brief Communication: Maternal Plasma Autoantibodies Screening in Fetal Down Syndrome Charkiewicz, Karol Zbucka-Kretowska, Monika Goscik, Joanna Wolczynski, Slawomir Lemancewicz, Adam Laudanski, Piotr J Immunol Res Research Article Imbalance in the metabolites levels which can potentially be related to certain fetal chromosomal abnormalities can stimulate mother's immune response to produce autoantibodies directed against proteins. The aim of the study was to determine the concentration of 9000 autoantibodies in maternal plasma to detect fetal Down syndrome. Method. We performed 190 amniocenteses and found 10 patients with confirmed fetal Down syndrome (15th–18th weeks of gestation). For the purpose of our control we chose 11 women without confirmed chromosomal aberration. To assess the expression of autoantibodies in the blood plasma, we used a protein microarray, which allows for simultaneous determination of 9000 proteins per sample. Results. We revealed 213 statistically significant autoantibodies, whose expression decreased or increased in the study group with fetal Down syndrome. The second step was to create a classifier of Down syndrome pregnancy, which includes 14 antibodies. The predictive value of the classifier (specificity and sensitivity) is 100%, classification errors, 0%, cross-validation errors, 0%. Conclusion. Our findings suggest that the autoantibodies may play a role in the pathophysiology of Down syndrome pregnancy. Defining their potential as biochemical markers of Down syndrome pregnancy requires further investigation on larger group of patients. Hindawi Publishing Corporation 2016 2016-03-06 /pmc/articles/PMC4799815/ /pubmed/27042674 http://dx.doi.org/10.1155/2016/9362169 Text en Copyright © 2016 Karol Charkiewicz et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Charkiewicz, Karol
Zbucka-Kretowska, Monika
Goscik, Joanna
Wolczynski, Slawomir
Lemancewicz, Adam
Laudanski, Piotr
Brief Communication: Maternal Plasma Autoantibodies Screening in Fetal Down Syndrome
title Brief Communication: Maternal Plasma Autoantibodies Screening in Fetal Down Syndrome
title_full Brief Communication: Maternal Plasma Autoantibodies Screening in Fetal Down Syndrome
title_fullStr Brief Communication: Maternal Plasma Autoantibodies Screening in Fetal Down Syndrome
title_full_unstemmed Brief Communication: Maternal Plasma Autoantibodies Screening in Fetal Down Syndrome
title_short Brief Communication: Maternal Plasma Autoantibodies Screening in Fetal Down Syndrome
title_sort brief communication: maternal plasma autoantibodies screening in fetal down syndrome
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4799815/
https://www.ncbi.nlm.nih.gov/pubmed/27042674
http://dx.doi.org/10.1155/2016/9362169
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