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Mesenchymal Stem Cell-Derived Microvesicles Support Ex Vivo Expansion of Cord Blood-Derived CD34(+) Cells

Mesenchymal stem cells (MSCs) are known to support the characteristic properties of hematopoietic stem and progenitor cells (HSPCs) in the bone marrow hematopoietic microenvironment. MSCs are used in coculture systems as a feeder layer for the ex vivo expansion of umbilical cord blood (CB) to increa...

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Autores principales: Xie, Hui, Sun, Li, Zhang, Liming, Liu, Teng, Chen, Li, Zhao, Aiqi, Lei, Qian, Gao, Fei, Zou, Ping, Li, Qiubai, Guo, An-yuan, Chen, Zhichao, Wang, Hongxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4799819/
https://www.ncbi.nlm.nih.gov/pubmed/27042183
http://dx.doi.org/10.1155/2016/6493241
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author Xie, Hui
Sun, Li
Zhang, Liming
Liu, Teng
Chen, Li
Zhao, Aiqi
Lei, Qian
Gao, Fei
Zou, Ping
Li, Qiubai
Guo, An-yuan
Chen, Zhichao
Wang, Hongxiang
author_facet Xie, Hui
Sun, Li
Zhang, Liming
Liu, Teng
Chen, Li
Zhao, Aiqi
Lei, Qian
Gao, Fei
Zou, Ping
Li, Qiubai
Guo, An-yuan
Chen, Zhichao
Wang, Hongxiang
author_sort Xie, Hui
collection PubMed
description Mesenchymal stem cells (MSCs) are known to support the characteristic properties of hematopoietic stem and progenitor cells (HSPCs) in the bone marrow hematopoietic microenvironment. MSCs are used in coculture systems as a feeder layer for the ex vivo expansion of umbilical cord blood (CB) to increase the relatively low number of HSPCs in CB. Findings increasingly suggest that MSC-derived microvesicles (MSC-MVs) play an important role in the biological functions of their parent cells. We speculate that MSC-MVs may recapitulate the hematopoiesis-supporting effects of their parent cells. In the current study, we found MSC-MVs containing microRNAs that are involved in the regulation of hematopoiesis. We also demonstrated that MSC-MVs could improve the expansion of CB-derived mononuclear cells and CD34(+) cells and generate a greater number of primitive progenitor cells in vitro. Additionally, when MSC-MVs were added to the CB-MSC coculture system, they could improve the hematopoiesis-supporting effects of MSCs. These findings highlight the role of MSC-MVs in the ex vivo expansion of CB, which may offer a promising therapeutic approach in CB transplantation.
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spelling pubmed-47998192016-04-03 Mesenchymal Stem Cell-Derived Microvesicles Support Ex Vivo Expansion of Cord Blood-Derived CD34(+) Cells Xie, Hui Sun, Li Zhang, Liming Liu, Teng Chen, Li Zhao, Aiqi Lei, Qian Gao, Fei Zou, Ping Li, Qiubai Guo, An-yuan Chen, Zhichao Wang, Hongxiang Stem Cells Int Research Article Mesenchymal stem cells (MSCs) are known to support the characteristic properties of hematopoietic stem and progenitor cells (HSPCs) in the bone marrow hematopoietic microenvironment. MSCs are used in coculture systems as a feeder layer for the ex vivo expansion of umbilical cord blood (CB) to increase the relatively low number of HSPCs in CB. Findings increasingly suggest that MSC-derived microvesicles (MSC-MVs) play an important role in the biological functions of their parent cells. We speculate that MSC-MVs may recapitulate the hematopoiesis-supporting effects of their parent cells. In the current study, we found MSC-MVs containing microRNAs that are involved in the regulation of hematopoiesis. We also demonstrated that MSC-MVs could improve the expansion of CB-derived mononuclear cells and CD34(+) cells and generate a greater number of primitive progenitor cells in vitro. Additionally, when MSC-MVs were added to the CB-MSC coculture system, they could improve the hematopoiesis-supporting effects of MSCs. These findings highlight the role of MSC-MVs in the ex vivo expansion of CB, which may offer a promising therapeutic approach in CB transplantation. Hindawi Publishing Corporation 2016 2016-03-06 /pmc/articles/PMC4799819/ /pubmed/27042183 http://dx.doi.org/10.1155/2016/6493241 Text en Copyright © 2016 Hui Xie et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Xie, Hui
Sun, Li
Zhang, Liming
Liu, Teng
Chen, Li
Zhao, Aiqi
Lei, Qian
Gao, Fei
Zou, Ping
Li, Qiubai
Guo, An-yuan
Chen, Zhichao
Wang, Hongxiang
Mesenchymal Stem Cell-Derived Microvesicles Support Ex Vivo Expansion of Cord Blood-Derived CD34(+) Cells
title Mesenchymal Stem Cell-Derived Microvesicles Support Ex Vivo Expansion of Cord Blood-Derived CD34(+) Cells
title_full Mesenchymal Stem Cell-Derived Microvesicles Support Ex Vivo Expansion of Cord Blood-Derived CD34(+) Cells
title_fullStr Mesenchymal Stem Cell-Derived Microvesicles Support Ex Vivo Expansion of Cord Blood-Derived CD34(+) Cells
title_full_unstemmed Mesenchymal Stem Cell-Derived Microvesicles Support Ex Vivo Expansion of Cord Blood-Derived CD34(+) Cells
title_short Mesenchymal Stem Cell-Derived Microvesicles Support Ex Vivo Expansion of Cord Blood-Derived CD34(+) Cells
title_sort mesenchymal stem cell-derived microvesicles support ex vivo expansion of cord blood-derived cd34(+) cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4799819/
https://www.ncbi.nlm.nih.gov/pubmed/27042183
http://dx.doi.org/10.1155/2016/6493241
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