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Development and characterization of two human triple‐negative breast cancer cell lines with highly tumorigenic and metastatic capabilities

Triple‐negative breast cancer (TNBC) is a group of cancer with high diversity, limited therapies, and poor prognosis. TNBC cell lines and animal models provide effective tools for studies and drug discovery. Here, we report the development of two TNBC cell lines (XtMCF and LmMCF) based on our existi...

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Autores principales: Su, Yanrong, Pogash, Thomas J., Nguyen, Theresa D., Russo, Jose
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4799943/
https://www.ncbi.nlm.nih.gov/pubmed/26775583
http://dx.doi.org/10.1002/cam4.616
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author Su, Yanrong
Pogash, Thomas J.
Nguyen, Theresa D.
Russo, Jose
author_facet Su, Yanrong
Pogash, Thomas J.
Nguyen, Theresa D.
Russo, Jose
author_sort Su, Yanrong
collection PubMed
description Triple‐negative breast cancer (TNBC) is a group of cancer with high diversity, limited therapies, and poor prognosis. TNBC cell lines and animal models provide effective tools for studies and drug discovery. Here, we report the development of two TNBC cell lines (XtMCF and LmMCF) based on our existing cell model that consists of normal breast epithelial cell line MCF10F, estradiol‐transformed cells trMCF, and Boyden chamber‐selected tumorigenic cells bsMCF. The XtMCF and LmMCF cell line were derived from xenograft and lung metastasis of bsMCF cells, respectively. The bsMCF, XtMCF, and LmMCF cells have undergone epithelial–mesenchymal transition (EMT), exhibiting a mesenchymal‐like feature. In vivo studies showed XtMCF and LmMCF cells were highly tumorigenic and metastatic. The injection of 5 × 10(4) cells to CB17/SCID mice mammary fat pad produced xenografts in 9/9 mice and tumors reached 10 millimeters in diameter in 5 weeks. The injection of 1 × 10(6) XtMCF or 8 × 10(4) LmMCF cells into the mice tail vein was sufficient to form extensive lung metastases in 4 weeks. The two new cell lines exhibited CD44(+)/CD49f(+) and CD44(+)/EpCAM (+) cancer stem cell (CSC) characteristics, and the EGF‐like domain of EpCAM was cleaved off. Together with the normal and early transformed counterparts, herein we provide a complete cancer model for the study of initiation, evolution, and identification of new therapeutics for TNBC. The finding that EGF‐like domain of EpCAM was cleaved off in cells which have undergone EMT suggests this cleavage may be involved in the EMT process and the cancer stem cell properties of these cells.
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spelling pubmed-47999432016-04-08 Development and characterization of two human triple‐negative breast cancer cell lines with highly tumorigenic and metastatic capabilities Su, Yanrong Pogash, Thomas J. Nguyen, Theresa D. Russo, Jose Cancer Med Cancer Biology Triple‐negative breast cancer (TNBC) is a group of cancer with high diversity, limited therapies, and poor prognosis. TNBC cell lines and animal models provide effective tools for studies and drug discovery. Here, we report the development of two TNBC cell lines (XtMCF and LmMCF) based on our existing cell model that consists of normal breast epithelial cell line MCF10F, estradiol‐transformed cells trMCF, and Boyden chamber‐selected tumorigenic cells bsMCF. The XtMCF and LmMCF cell line were derived from xenograft and lung metastasis of bsMCF cells, respectively. The bsMCF, XtMCF, and LmMCF cells have undergone epithelial–mesenchymal transition (EMT), exhibiting a mesenchymal‐like feature. In vivo studies showed XtMCF and LmMCF cells were highly tumorigenic and metastatic. The injection of 5 × 10(4) cells to CB17/SCID mice mammary fat pad produced xenografts in 9/9 mice and tumors reached 10 millimeters in diameter in 5 weeks. The injection of 1 × 10(6) XtMCF or 8 × 10(4) LmMCF cells into the mice tail vein was sufficient to form extensive lung metastases in 4 weeks. The two new cell lines exhibited CD44(+)/CD49f(+) and CD44(+)/EpCAM (+) cancer stem cell (CSC) characteristics, and the EGF‐like domain of EpCAM was cleaved off. Together with the normal and early transformed counterparts, herein we provide a complete cancer model for the study of initiation, evolution, and identification of new therapeutics for TNBC. The finding that EGF‐like domain of EpCAM was cleaved off in cells which have undergone EMT suggests this cleavage may be involved in the EMT process and the cancer stem cell properties of these cells. John Wiley and Sons Inc. 2016-01-18 /pmc/articles/PMC4799943/ /pubmed/26775583 http://dx.doi.org/10.1002/cam4.616 Text en © 2016 The Authors. Cancer Medicine published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Cancer Biology
Su, Yanrong
Pogash, Thomas J.
Nguyen, Theresa D.
Russo, Jose
Development and characterization of two human triple‐negative breast cancer cell lines with highly tumorigenic and metastatic capabilities
title Development and characterization of two human triple‐negative breast cancer cell lines with highly tumorigenic and metastatic capabilities
title_full Development and characterization of two human triple‐negative breast cancer cell lines with highly tumorigenic and metastatic capabilities
title_fullStr Development and characterization of two human triple‐negative breast cancer cell lines with highly tumorigenic and metastatic capabilities
title_full_unstemmed Development and characterization of two human triple‐negative breast cancer cell lines with highly tumorigenic and metastatic capabilities
title_short Development and characterization of two human triple‐negative breast cancer cell lines with highly tumorigenic and metastatic capabilities
title_sort development and characterization of two human triple‐negative breast cancer cell lines with highly tumorigenic and metastatic capabilities
topic Cancer Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4799943/
https://www.ncbi.nlm.nih.gov/pubmed/26775583
http://dx.doi.org/10.1002/cam4.616
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