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Cyclic Stretch Facilitates Myogenesis in C2C12 Myoblasts and Rescues Thiazolidinedione-Inhibited Myotube Formation

Thiazolidinedione (TZD), a specific peroxisome proliferator-activated receptor γ (PPARγ) agonist, was developed to control blood glucose in diabetes patients. However, several side effects were reported that increased the risk of heart failure. We used C2C12 myoblasts to investigate the role of PPAR...

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Autores principales: Chang, Ya-Ju, Chen, Yun-Ju, Huang, Chia-Wei, Fan, Shih-Chen, Huang, Bu-Miin, Chang, Wen-Tsan, Tsai, Yau-Sheng, Su, Fong-Chin, Wu, Chia-Ching
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4800178/
https://www.ncbi.nlm.nih.gov/pubmed/27047938
http://dx.doi.org/10.3389/fbioe.2016.00027
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author Chang, Ya-Ju
Chen, Yun-Ju
Huang, Chia-Wei
Fan, Shih-Chen
Huang, Bu-Miin
Chang, Wen-Tsan
Tsai, Yau-Sheng
Su, Fong-Chin
Wu, Chia-Ching
author_facet Chang, Ya-Ju
Chen, Yun-Ju
Huang, Chia-Wei
Fan, Shih-Chen
Huang, Bu-Miin
Chang, Wen-Tsan
Tsai, Yau-Sheng
Su, Fong-Chin
Wu, Chia-Ching
author_sort Chang, Ya-Ju
collection PubMed
description Thiazolidinedione (TZD), a specific peroxisome proliferator-activated receptor γ (PPARγ) agonist, was developed to control blood glucose in diabetes patients. However, several side effects were reported that increased the risk of heart failure. We used C2C12 myoblasts to investigate the role of PPARs and their transcriptional activity during myotube formation. The role of mechanical stretch during myogenesis was also explored by applying cyclic stretch to the differentiating C2C12 myoblasts with 10% strain deformation at 1 Hz. The myogenesis medium (MM), composed of Dulbecco’s modified Eagle’s medium with 2% horse serum, facilitated myotube formation with increased myosin heavy chain and α-smooth muscle actin (α-SMA) protein expression. The PPARγ protein and PPAR response element (PPRE) promoter activity decreased during MM induction. Cyclic stretch further facilitated the myogenesis in MM with increased α-SMA and decreased PPARγ protein expression and inhibited PPRE promoter activity. Adding a PPARγ agonist (TZD) to the MM stopped the myogenesis and restored the PPRE promoter activity, whereas a PPARγ antagonist (GW9662) significantly increased the myotube number and length. During the myogenesis induction, application of cyclic stretch rescued the inhibitory effects of TZD. These results provide novel perspectives for mechanical stretch to interplay and rescue the dysfunction of myogenesis with the involvement of PPARγ and its target drugs.
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spelling pubmed-48001782016-04-04 Cyclic Stretch Facilitates Myogenesis in C2C12 Myoblasts and Rescues Thiazolidinedione-Inhibited Myotube Formation Chang, Ya-Ju Chen, Yun-Ju Huang, Chia-Wei Fan, Shih-Chen Huang, Bu-Miin Chang, Wen-Tsan Tsai, Yau-Sheng Su, Fong-Chin Wu, Chia-Ching Front Bioeng Biotechnol Bioengineering and Biotechnology Thiazolidinedione (TZD), a specific peroxisome proliferator-activated receptor γ (PPARγ) agonist, was developed to control blood glucose in diabetes patients. However, several side effects were reported that increased the risk of heart failure. We used C2C12 myoblasts to investigate the role of PPARs and their transcriptional activity during myotube formation. The role of mechanical stretch during myogenesis was also explored by applying cyclic stretch to the differentiating C2C12 myoblasts with 10% strain deformation at 1 Hz. The myogenesis medium (MM), composed of Dulbecco’s modified Eagle’s medium with 2% horse serum, facilitated myotube formation with increased myosin heavy chain and α-smooth muscle actin (α-SMA) protein expression. The PPARγ protein and PPAR response element (PPRE) promoter activity decreased during MM induction. Cyclic stretch further facilitated the myogenesis in MM with increased α-SMA and decreased PPARγ protein expression and inhibited PPRE promoter activity. Adding a PPARγ agonist (TZD) to the MM stopped the myogenesis and restored the PPRE promoter activity, whereas a PPARγ antagonist (GW9662) significantly increased the myotube number and length. During the myogenesis induction, application of cyclic stretch rescued the inhibitory effects of TZD. These results provide novel perspectives for mechanical stretch to interplay and rescue the dysfunction of myogenesis with the involvement of PPARγ and its target drugs. Frontiers Media S.A. 2016-03-21 /pmc/articles/PMC4800178/ /pubmed/27047938 http://dx.doi.org/10.3389/fbioe.2016.00027 Text en Copyright © 2016 Chang, Chen, Huang, Fan, Huang, Chang, Tsai, Su and Wu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Chang, Ya-Ju
Chen, Yun-Ju
Huang, Chia-Wei
Fan, Shih-Chen
Huang, Bu-Miin
Chang, Wen-Tsan
Tsai, Yau-Sheng
Su, Fong-Chin
Wu, Chia-Ching
Cyclic Stretch Facilitates Myogenesis in C2C12 Myoblasts and Rescues Thiazolidinedione-Inhibited Myotube Formation
title Cyclic Stretch Facilitates Myogenesis in C2C12 Myoblasts and Rescues Thiazolidinedione-Inhibited Myotube Formation
title_full Cyclic Stretch Facilitates Myogenesis in C2C12 Myoblasts and Rescues Thiazolidinedione-Inhibited Myotube Formation
title_fullStr Cyclic Stretch Facilitates Myogenesis in C2C12 Myoblasts and Rescues Thiazolidinedione-Inhibited Myotube Formation
title_full_unstemmed Cyclic Stretch Facilitates Myogenesis in C2C12 Myoblasts and Rescues Thiazolidinedione-Inhibited Myotube Formation
title_short Cyclic Stretch Facilitates Myogenesis in C2C12 Myoblasts and Rescues Thiazolidinedione-Inhibited Myotube Formation
title_sort cyclic stretch facilitates myogenesis in c2c12 myoblasts and rescues thiazolidinedione-inhibited myotube formation
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4800178/
https://www.ncbi.nlm.nih.gov/pubmed/27047938
http://dx.doi.org/10.3389/fbioe.2016.00027
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