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Rapid bacterial antibiotic susceptibility test based on simple surface-enhanced Raman spectroscopic biomarkers
Rapid bacterial antibiotic susceptibility test (AST) and minimum inhibitory concentration (MIC) measurement are important to help reduce the widespread misuse of antibiotics and alleviate the growing drug-resistance problem. We discovered that, when a susceptible strain of Staphylococcus aureus or E...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4800312/ https://www.ncbi.nlm.nih.gov/pubmed/26997474 http://dx.doi.org/10.1038/srep23375 |
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author | Liu, Chia-Ying Han, Yin-Yi Shih, Po-Han Lian, Wei-Nan Wang, Huai-Hsien Lin, Chi-Hung Hsueh, Po-Ren Wang, Juen-Kai Wang, Yuh-Lin |
author_facet | Liu, Chia-Ying Han, Yin-Yi Shih, Po-Han Lian, Wei-Nan Wang, Huai-Hsien Lin, Chi-Hung Hsueh, Po-Ren Wang, Juen-Kai Wang, Yuh-Lin |
author_sort | Liu, Chia-Ying |
collection | PubMed |
description | Rapid bacterial antibiotic susceptibility test (AST) and minimum inhibitory concentration (MIC) measurement are important to help reduce the widespread misuse of antibiotics and alleviate the growing drug-resistance problem. We discovered that, when a susceptible strain of Staphylococcus aureus or Escherichia coli is exposed to an antibiotic, the intensity of specific biomarkers in its surface-enhanced Raman scattering (SERS) spectra drops evidently in two hours. The discovery has been exploited for rapid AST and MIC determination of methicillin-susceptible S. aureus and wild-type E. coli as well as clinical isolates. The results obtained by this SERS-AST method were consistent with that by the standard incubation-based method, indicating its high potential to supplement or replace existing time-consuming methods and help mitigate the challenge of drug resistance in clinical microbiology. |
format | Online Article Text |
id | pubmed-4800312 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48003122016-03-22 Rapid bacterial antibiotic susceptibility test based on simple surface-enhanced Raman spectroscopic biomarkers Liu, Chia-Ying Han, Yin-Yi Shih, Po-Han Lian, Wei-Nan Wang, Huai-Hsien Lin, Chi-Hung Hsueh, Po-Ren Wang, Juen-Kai Wang, Yuh-Lin Sci Rep Article Rapid bacterial antibiotic susceptibility test (AST) and minimum inhibitory concentration (MIC) measurement are important to help reduce the widespread misuse of antibiotics and alleviate the growing drug-resistance problem. We discovered that, when a susceptible strain of Staphylococcus aureus or Escherichia coli is exposed to an antibiotic, the intensity of specific biomarkers in its surface-enhanced Raman scattering (SERS) spectra drops evidently in two hours. The discovery has been exploited for rapid AST and MIC determination of methicillin-susceptible S. aureus and wild-type E. coli as well as clinical isolates. The results obtained by this SERS-AST method were consistent with that by the standard incubation-based method, indicating its high potential to supplement or replace existing time-consuming methods and help mitigate the challenge of drug resistance in clinical microbiology. Nature Publishing Group 2016-03-21 /pmc/articles/PMC4800312/ /pubmed/26997474 http://dx.doi.org/10.1038/srep23375 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Liu, Chia-Ying Han, Yin-Yi Shih, Po-Han Lian, Wei-Nan Wang, Huai-Hsien Lin, Chi-Hung Hsueh, Po-Ren Wang, Juen-Kai Wang, Yuh-Lin Rapid bacterial antibiotic susceptibility test based on simple surface-enhanced Raman spectroscopic biomarkers |
title | Rapid bacterial antibiotic susceptibility test based on simple surface-enhanced Raman spectroscopic biomarkers |
title_full | Rapid bacterial antibiotic susceptibility test based on simple surface-enhanced Raman spectroscopic biomarkers |
title_fullStr | Rapid bacterial antibiotic susceptibility test based on simple surface-enhanced Raman spectroscopic biomarkers |
title_full_unstemmed | Rapid bacterial antibiotic susceptibility test based on simple surface-enhanced Raman spectroscopic biomarkers |
title_short | Rapid bacterial antibiotic susceptibility test based on simple surface-enhanced Raman spectroscopic biomarkers |
title_sort | rapid bacterial antibiotic susceptibility test based on simple surface-enhanced raman spectroscopic biomarkers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4800312/ https://www.ncbi.nlm.nih.gov/pubmed/26997474 http://dx.doi.org/10.1038/srep23375 |
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