Cargando…

Fabrication, characterization, and biological assessment of multilayer laminin γ2 DNA coatings on titanium surfaces

The purpose of this work was to fabricate a multilayer laminin γ2 DNA coating on a titanium surface and evaluate its biological properties. A multilayer laminin γ2 DNA coating was fabricated on titanium using a layer-by-layer assembly technique. The rate of coating degradation was evaluated by detec...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Guoli, Zhang, Jing, Dong, Wenjing, Liu, Li, Shi, Jue, Wang, Huiming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4800452/
https://www.ncbi.nlm.nih.gov/pubmed/26996815
http://dx.doi.org/10.1038/srep23423
_version_ 1782422489697091584
author Yang, Guoli
Zhang, Jing
Dong, Wenjing
Liu, Li
Shi, Jue
Wang, Huiming
author_facet Yang, Guoli
Zhang, Jing
Dong, Wenjing
Liu, Li
Shi, Jue
Wang, Huiming
author_sort Yang, Guoli
collection PubMed
description The purpose of this work was to fabricate a multilayer laminin γ2 DNA coating on a titanium surface and evaluate its biological properties. A multilayer laminin γ2 DNA coating was fabricated on titanium using a layer-by-layer assembly technique. The rate of coating degradation was evaluated by detecting the amount of cDNA remaining. Surface analysis using X-ray photoelectron spectroscopy, atomic force microscopy, and surface contact angle measurements revealed the multilayer structure to consist of cationic lipid and confirmed that a laminin γ2 DNA layer could be fabricated on titanium via the layer-by-layer assembly process. The transfection efficiency was highest for five layers in the multilayer structure. HEK293 cells cultured on the multilayer films displayed significantly higher adhesion activity than the control group. The expression of laminin γ2 and the co-localization of integrin β4 and plectin were more obvious in HN4 cells cultured on the multilayer laminin γ2 DNA coating, while weak immunoreactivities were observed in the control group. We concluded that the DNA-loaded multilayer provided a surface with good biocompatibility and that the multilayer laminin γ2 DNA coating might be effective in improving cell adhesion and the formation of hemidesmosomes on titanium surfaces.
format Online
Article
Text
id pubmed-4800452
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-48004522016-03-22 Fabrication, characterization, and biological assessment of multilayer laminin γ2 DNA coatings on titanium surfaces Yang, Guoli Zhang, Jing Dong, Wenjing Liu, Li Shi, Jue Wang, Huiming Sci Rep Article The purpose of this work was to fabricate a multilayer laminin γ2 DNA coating on a titanium surface and evaluate its biological properties. A multilayer laminin γ2 DNA coating was fabricated on titanium using a layer-by-layer assembly technique. The rate of coating degradation was evaluated by detecting the amount of cDNA remaining. Surface analysis using X-ray photoelectron spectroscopy, atomic force microscopy, and surface contact angle measurements revealed the multilayer structure to consist of cationic lipid and confirmed that a laminin γ2 DNA layer could be fabricated on titanium via the layer-by-layer assembly process. The transfection efficiency was highest for five layers in the multilayer structure. HEK293 cells cultured on the multilayer films displayed significantly higher adhesion activity than the control group. The expression of laminin γ2 and the co-localization of integrin β4 and plectin were more obvious in HN4 cells cultured on the multilayer laminin γ2 DNA coating, while weak immunoreactivities were observed in the control group. We concluded that the DNA-loaded multilayer provided a surface with good biocompatibility and that the multilayer laminin γ2 DNA coating might be effective in improving cell adhesion and the formation of hemidesmosomes on titanium surfaces. Nature Publishing Group 2016-03-21 /pmc/articles/PMC4800452/ /pubmed/26996815 http://dx.doi.org/10.1038/srep23423 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Yang, Guoli
Zhang, Jing
Dong, Wenjing
Liu, Li
Shi, Jue
Wang, Huiming
Fabrication, characterization, and biological assessment of multilayer laminin γ2 DNA coatings on titanium surfaces
title Fabrication, characterization, and biological assessment of multilayer laminin γ2 DNA coatings on titanium surfaces
title_full Fabrication, characterization, and biological assessment of multilayer laminin γ2 DNA coatings on titanium surfaces
title_fullStr Fabrication, characterization, and biological assessment of multilayer laminin γ2 DNA coatings on titanium surfaces
title_full_unstemmed Fabrication, characterization, and biological assessment of multilayer laminin γ2 DNA coatings on titanium surfaces
title_short Fabrication, characterization, and biological assessment of multilayer laminin γ2 DNA coatings on titanium surfaces
title_sort fabrication, characterization, and biological assessment of multilayer laminin γ2 dna coatings on titanium surfaces
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4800452/
https://www.ncbi.nlm.nih.gov/pubmed/26996815
http://dx.doi.org/10.1038/srep23423
work_keys_str_mv AT yangguoli fabricationcharacterizationandbiologicalassessmentofmultilayerlamining2dnacoatingsontitaniumsurfaces
AT zhangjing fabricationcharacterizationandbiologicalassessmentofmultilayerlamining2dnacoatingsontitaniumsurfaces
AT dongwenjing fabricationcharacterizationandbiologicalassessmentofmultilayerlamining2dnacoatingsontitaniumsurfaces
AT liuli fabricationcharacterizationandbiologicalassessmentofmultilayerlamining2dnacoatingsontitaniumsurfaces
AT shijue fabricationcharacterizationandbiologicalassessmentofmultilayerlamining2dnacoatingsontitaniumsurfaces
AT wanghuiming fabricationcharacterizationandbiologicalassessmentofmultilayerlamining2dnacoatingsontitaniumsurfaces