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Thermodynamic Features of Structural Motifs Formed by β-L-RNA
This is the first report to provide comprehensive thermodynamic and structural data concerning duplex, hairpin, quadruplex and i-motif structures in β-L-RNA series. Herein we confirm that, within the limits of experimental error, the thermodynamic stability of enantiomeric structural motifs is the s...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4801053/ https://www.ncbi.nlm.nih.gov/pubmed/26908023 http://dx.doi.org/10.1371/journal.pone.0149478 |
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author | Szabat, Marta Gudanis, Dorota Kotkowiak, Weronika Gdaniec, Zofia Kierzek, Ryszard Pasternak, Anna |
author_facet | Szabat, Marta Gudanis, Dorota Kotkowiak, Weronika Gdaniec, Zofia Kierzek, Ryszard Pasternak, Anna |
author_sort | Szabat, Marta |
collection | PubMed |
description | This is the first report to provide comprehensive thermodynamic and structural data concerning duplex, hairpin, quadruplex and i-motif structures in β-L-RNA series. Herein we confirm that, within the limits of experimental error, the thermodynamic stability of enantiomeric structural motifs is the same as that of naturally occurring D-RNA counterparts. In addition, formation of D-RNA/L-RNA heterochiral duplexes is also observed; however, their thermodynamic stability is significantly reduced in reference to homochiral D-RNA duplexes. The presence of three locked nucleic acid (LNA) residues within the D-RNA strand diminishes the negative effect of the enantiomeric, complementary L-RNA strand in the formation of heterochiral RNA duplexes. Similar behavior is also observed for heterochiral LNA-2′-O-methyl-D-RNA/L-RNA duplexes. The formation of heterochiral duplexes was confirmed by (1)H NMR spectroscopy. The CD curves of homochiral L-RNA structural motifs are always reversed, whereas CD curves of heterochiral duplexes present individual features dependent on the composition of chiral strands. |
format | Online Article Text |
id | pubmed-4801053 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-48010532016-03-23 Thermodynamic Features of Structural Motifs Formed by β-L-RNA Szabat, Marta Gudanis, Dorota Kotkowiak, Weronika Gdaniec, Zofia Kierzek, Ryszard Pasternak, Anna PLoS One Research Article This is the first report to provide comprehensive thermodynamic and structural data concerning duplex, hairpin, quadruplex and i-motif structures in β-L-RNA series. Herein we confirm that, within the limits of experimental error, the thermodynamic stability of enantiomeric structural motifs is the same as that of naturally occurring D-RNA counterparts. In addition, formation of D-RNA/L-RNA heterochiral duplexes is also observed; however, their thermodynamic stability is significantly reduced in reference to homochiral D-RNA duplexes. The presence of three locked nucleic acid (LNA) residues within the D-RNA strand diminishes the negative effect of the enantiomeric, complementary L-RNA strand in the formation of heterochiral RNA duplexes. Similar behavior is also observed for heterochiral LNA-2′-O-methyl-D-RNA/L-RNA duplexes. The formation of heterochiral duplexes was confirmed by (1)H NMR spectroscopy. The CD curves of homochiral L-RNA structural motifs are always reversed, whereas CD curves of heterochiral duplexes present individual features dependent on the composition of chiral strands. Public Library of Science 2016-02-23 /pmc/articles/PMC4801053/ /pubmed/26908023 http://dx.doi.org/10.1371/journal.pone.0149478 Text en © 2016 Szabat et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Szabat, Marta Gudanis, Dorota Kotkowiak, Weronika Gdaniec, Zofia Kierzek, Ryszard Pasternak, Anna Thermodynamic Features of Structural Motifs Formed by β-L-RNA |
title | Thermodynamic Features of Structural Motifs Formed by β-L-RNA |
title_full | Thermodynamic Features of Structural Motifs Formed by β-L-RNA |
title_fullStr | Thermodynamic Features of Structural Motifs Formed by β-L-RNA |
title_full_unstemmed | Thermodynamic Features of Structural Motifs Formed by β-L-RNA |
title_short | Thermodynamic Features of Structural Motifs Formed by β-L-RNA |
title_sort | thermodynamic features of structural motifs formed by β-l-rna |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4801053/ https://www.ncbi.nlm.nih.gov/pubmed/26908023 http://dx.doi.org/10.1371/journal.pone.0149478 |
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