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A Complete Optical Sensor System Based on a POF-SPR Platform and a Thermo-Stabilized Flow Cell for Biochemical Applications
An optical sensor platform based on surface plasmon resonance (SPR) in a plastic optical fiber (POF) integrated into a thermo-stabilized flow cell for biochemical sensing applications is proposed. This device has been realized and experimentally tested by using a classic receptor-analyte assay. For...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4801573/ https://www.ncbi.nlm.nih.gov/pubmed/26861328 http://dx.doi.org/10.3390/s16020196 |
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author | Cennamo, Nunzio Chiavaioli, Francesco Trono, Cosimo Tombelli, Sara Giannetti, Ambra Baldini, Francesco Zeni, Luigi |
author_facet | Cennamo, Nunzio Chiavaioli, Francesco Trono, Cosimo Tombelli, Sara Giannetti, Ambra Baldini, Francesco Zeni, Luigi |
author_sort | Cennamo, Nunzio |
collection | PubMed |
description | An optical sensor platform based on surface plasmon resonance (SPR) in a plastic optical fiber (POF) integrated into a thermo-stabilized flow cell for biochemical sensing applications is proposed. This device has been realized and experimentally tested by using a classic receptor-analyte assay. For this purpose, the gold surface of the POF was chemically modified through the formation of a self-assembling monolayer. The surface robustness of the POF-SPR platform has been tested for the first time thanks to the flow cell. The experimental results show that the proposed device can be successfully used for label-free biochemical sensing. The final goal of this work is to achieve a complete, small-size, simple to use and low cost optical sensor system. The whole system with the flow cell and the optical sensor are extensively described, together with the experimental results obtained with an immunoglobulin G (IgG)/anti-IgG assay. |
format | Online Article Text |
id | pubmed-4801573 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-48015732016-03-25 A Complete Optical Sensor System Based on a POF-SPR Platform and a Thermo-Stabilized Flow Cell for Biochemical Applications Cennamo, Nunzio Chiavaioli, Francesco Trono, Cosimo Tombelli, Sara Giannetti, Ambra Baldini, Francesco Zeni, Luigi Sensors (Basel) Article An optical sensor platform based on surface plasmon resonance (SPR) in a plastic optical fiber (POF) integrated into a thermo-stabilized flow cell for biochemical sensing applications is proposed. This device has been realized and experimentally tested by using a classic receptor-analyte assay. For this purpose, the gold surface of the POF was chemically modified through the formation of a self-assembling monolayer. The surface robustness of the POF-SPR platform has been tested for the first time thanks to the flow cell. The experimental results show that the proposed device can be successfully used for label-free biochemical sensing. The final goal of this work is to achieve a complete, small-size, simple to use and low cost optical sensor system. The whole system with the flow cell and the optical sensor are extensively described, together with the experimental results obtained with an immunoglobulin G (IgG)/anti-IgG assay. MDPI 2016-02-05 /pmc/articles/PMC4801573/ /pubmed/26861328 http://dx.doi.org/10.3390/s16020196 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cennamo, Nunzio Chiavaioli, Francesco Trono, Cosimo Tombelli, Sara Giannetti, Ambra Baldini, Francesco Zeni, Luigi A Complete Optical Sensor System Based on a POF-SPR Platform and a Thermo-Stabilized Flow Cell for Biochemical Applications |
title | A Complete Optical Sensor System Based on a POF-SPR Platform and a Thermo-Stabilized Flow Cell for Biochemical Applications |
title_full | A Complete Optical Sensor System Based on a POF-SPR Platform and a Thermo-Stabilized Flow Cell for Biochemical Applications |
title_fullStr | A Complete Optical Sensor System Based on a POF-SPR Platform and a Thermo-Stabilized Flow Cell for Biochemical Applications |
title_full_unstemmed | A Complete Optical Sensor System Based on a POF-SPR Platform and a Thermo-Stabilized Flow Cell for Biochemical Applications |
title_short | A Complete Optical Sensor System Based on a POF-SPR Platform and a Thermo-Stabilized Flow Cell for Biochemical Applications |
title_sort | complete optical sensor system based on a pof-spr platform and a thermo-stabilized flow cell for biochemical applications |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4801573/ https://www.ncbi.nlm.nih.gov/pubmed/26861328 http://dx.doi.org/10.3390/s16020196 |
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