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A Complete Optical Sensor System Based on a POF-SPR Platform and a Thermo-Stabilized Flow Cell for Biochemical Applications

An optical sensor platform based on surface plasmon resonance (SPR) in a plastic optical fiber (POF) integrated into a thermo-stabilized flow cell for biochemical sensing applications is proposed. This device has been realized and experimentally tested by using a classic receptor-analyte assay. For...

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Autores principales: Cennamo, Nunzio, Chiavaioli, Francesco, Trono, Cosimo, Tombelli, Sara, Giannetti, Ambra, Baldini, Francesco, Zeni, Luigi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4801573/
https://www.ncbi.nlm.nih.gov/pubmed/26861328
http://dx.doi.org/10.3390/s16020196
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author Cennamo, Nunzio
Chiavaioli, Francesco
Trono, Cosimo
Tombelli, Sara
Giannetti, Ambra
Baldini, Francesco
Zeni, Luigi
author_facet Cennamo, Nunzio
Chiavaioli, Francesco
Trono, Cosimo
Tombelli, Sara
Giannetti, Ambra
Baldini, Francesco
Zeni, Luigi
author_sort Cennamo, Nunzio
collection PubMed
description An optical sensor platform based on surface plasmon resonance (SPR) in a plastic optical fiber (POF) integrated into a thermo-stabilized flow cell for biochemical sensing applications is proposed. This device has been realized and experimentally tested by using a classic receptor-analyte assay. For this purpose, the gold surface of the POF was chemically modified through the formation of a self-assembling monolayer. The surface robustness of the POF-SPR platform has been tested for the first time thanks to the flow cell. The experimental results show that the proposed device can be successfully used for label-free biochemical sensing. The final goal of this work is to achieve a complete, small-size, simple to use and low cost optical sensor system. The whole system with the flow cell and the optical sensor are extensively described, together with the experimental results obtained with an immunoglobulin G (IgG)/anti-IgG assay.
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spelling pubmed-48015732016-03-25 A Complete Optical Sensor System Based on a POF-SPR Platform and a Thermo-Stabilized Flow Cell for Biochemical Applications Cennamo, Nunzio Chiavaioli, Francesco Trono, Cosimo Tombelli, Sara Giannetti, Ambra Baldini, Francesco Zeni, Luigi Sensors (Basel) Article An optical sensor platform based on surface plasmon resonance (SPR) in a plastic optical fiber (POF) integrated into a thermo-stabilized flow cell for biochemical sensing applications is proposed. This device has been realized and experimentally tested by using a classic receptor-analyte assay. For this purpose, the gold surface of the POF was chemically modified through the formation of a self-assembling monolayer. The surface robustness of the POF-SPR platform has been tested for the first time thanks to the flow cell. The experimental results show that the proposed device can be successfully used for label-free biochemical sensing. The final goal of this work is to achieve a complete, small-size, simple to use and low cost optical sensor system. The whole system with the flow cell and the optical sensor are extensively described, together with the experimental results obtained with an immunoglobulin G (IgG)/anti-IgG assay. MDPI 2016-02-05 /pmc/articles/PMC4801573/ /pubmed/26861328 http://dx.doi.org/10.3390/s16020196 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cennamo, Nunzio
Chiavaioli, Francesco
Trono, Cosimo
Tombelli, Sara
Giannetti, Ambra
Baldini, Francesco
Zeni, Luigi
A Complete Optical Sensor System Based on a POF-SPR Platform and a Thermo-Stabilized Flow Cell for Biochemical Applications
title A Complete Optical Sensor System Based on a POF-SPR Platform and a Thermo-Stabilized Flow Cell for Biochemical Applications
title_full A Complete Optical Sensor System Based on a POF-SPR Platform and a Thermo-Stabilized Flow Cell for Biochemical Applications
title_fullStr A Complete Optical Sensor System Based on a POF-SPR Platform and a Thermo-Stabilized Flow Cell for Biochemical Applications
title_full_unstemmed A Complete Optical Sensor System Based on a POF-SPR Platform and a Thermo-Stabilized Flow Cell for Biochemical Applications
title_short A Complete Optical Sensor System Based on a POF-SPR Platform and a Thermo-Stabilized Flow Cell for Biochemical Applications
title_sort complete optical sensor system based on a pof-spr platform and a thermo-stabilized flow cell for biochemical applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4801573/
https://www.ncbi.nlm.nih.gov/pubmed/26861328
http://dx.doi.org/10.3390/s16020196
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