Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization

This article reviews recent developments in microfluidic systems enabling high-throughput characterization of single-cell proteins. Four key perspectives of microfluidic platforms are included in this review: (1) microfluidic fluorescent flow cytometry; (2) droplet based microfluidic flow cytometry;...

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Detalles Bibliográficos
Autores principales: Fan, Beiyuan, Li, Xiufeng, Chen, Deyong, Peng, Hongshang, Wang, Junbo, Chen, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4801608/
https://www.ncbi.nlm.nih.gov/pubmed/26891303
http://dx.doi.org/10.3390/s16020232
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author Fan, Beiyuan
Li, Xiufeng
Chen, Deyong
Peng, Hongshang
Wang, Junbo
Chen, Jian
author_facet Fan, Beiyuan
Li, Xiufeng
Chen, Deyong
Peng, Hongshang
Wang, Junbo
Chen, Jian
author_sort Fan, Beiyuan
collection PubMed
description This article reviews recent developments in microfluidic systems enabling high-throughput characterization of single-cell proteins. Four key perspectives of microfluidic platforms are included in this review: (1) microfluidic fluorescent flow cytometry; (2) droplet based microfluidic flow cytometry; (3) large-array micro wells (microengraving); and (4) large-array micro chambers (barcode microchips). We examine the advantages and limitations of each technique and discuss future research opportunities by focusing on three key performance parameters (absolute quantification, sensitivity, and throughput).
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spelling pubmed-48016082016-03-25 Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization Fan, Beiyuan Li, Xiufeng Chen, Deyong Peng, Hongshang Wang, Junbo Chen, Jian Sensors (Basel) Review This article reviews recent developments in microfluidic systems enabling high-throughput characterization of single-cell proteins. Four key perspectives of microfluidic platforms are included in this review: (1) microfluidic fluorescent flow cytometry; (2) droplet based microfluidic flow cytometry; (3) large-array micro wells (microengraving); and (4) large-array micro chambers (barcode microchips). We examine the advantages and limitations of each technique and discuss future research opportunities by focusing on three key performance parameters (absolute quantification, sensitivity, and throughput). MDPI 2016-02-16 /pmc/articles/PMC4801608/ /pubmed/26891303 http://dx.doi.org/10.3390/s16020232 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Fan, Beiyuan
Li, Xiufeng
Chen, Deyong
Peng, Hongshang
Wang, Junbo
Chen, Jian
Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization
title Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization
title_full Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization
title_fullStr Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization
title_full_unstemmed Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization
title_short Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization
title_sort development of microfluidic systems enabling high-throughput single-cell protein characterization
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4801608/
https://www.ncbi.nlm.nih.gov/pubmed/26891303
http://dx.doi.org/10.3390/s16020232
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