Cargando…

The Rice TCM5 Gene Encoding a Novel Deg Protease Protein is Essential for Chloroplast Development under High Temperatures

BACKGROUND: High temperature affects a broad spectrum of cellular components and metabolism in plants. The Deg/HtrA family of ATP-independent serine endopeptidases is present in nearly all organisms. Deg proteases are required for the survival of Escherichia coli at high temperatures. However, it is...

Descripción completa

Detalles Bibliográficos
Autores principales: Zheng, Kailun, Zhao, Jian, Lin, Dongzhi, Chen, Jiaying, Xu, Jianlong, Zhou, Hua, Teng, Sheng, Dong, Yanjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4801845/
https://www.ncbi.nlm.nih.gov/pubmed/27000876
http://dx.doi.org/10.1186/s12284-016-0086-5
_version_ 1782422625482440704
author Zheng, Kailun
Zhao, Jian
Lin, Dongzhi
Chen, Jiaying
Xu, Jianlong
Zhou, Hua
Teng, Sheng
Dong, Yanjun
author_facet Zheng, Kailun
Zhao, Jian
Lin, Dongzhi
Chen, Jiaying
Xu, Jianlong
Zhou, Hua
Teng, Sheng
Dong, Yanjun
author_sort Zheng, Kailun
collection PubMed
description BACKGROUND: High temperature affects a broad spectrum of cellular components and metabolism in plants. The Deg/HtrA family of ATP-independent serine endopeptidases is present in nearly all organisms. Deg proteases are required for the survival of Escherichia coli at high temperatures. However, it is still unclear whether rice Deg proteases are required for chloroplast development under high temperatures. RESULTS: In this study, we reported the first rice deg mutant tcm5 (thermo-sensitive chlorophyll-deficient mutant 5) that has an albino phenotype, defective chloroplasts and could not survive after the 4–5 leaf seedling stage when grown at high temperature (32 °C). However, when grown at low temperatures (20 °C), tcm5 has a normal phenotype. Map-based cloning showed that TCM5 encoding a chloroplast-targeted Deg protease protein. The TCM5 transcripts were highly expressed in all green tissues and undetectable in other tissues, showing the tissue-specific expression. In tcm5 mutants grown at high temperatures, the transcript levels of certain genes associated with chloroplast development especially PSII-associated genes were severely affected, but recovered to normal levels at low temperatures. These results showed important role of TCM5 for chloroplast development under high temperatures. CONCLUSIONS: The TCM5 encodes chloroplast-targeted Deg protease protein which is important for chloroplast development and the maintenance of PSII function and its disruption would lead to a defective chloroplast and affected expression levels of genes associated with chloroplast development and photosynthesis at early rice seedling stage under high temperatures. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12284-016-0086-5) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-4801845
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Springer US
record_format MEDLINE/PubMed
spelling pubmed-48018452016-04-09 The Rice TCM5 Gene Encoding a Novel Deg Protease Protein is Essential for Chloroplast Development under High Temperatures Zheng, Kailun Zhao, Jian Lin, Dongzhi Chen, Jiaying Xu, Jianlong Zhou, Hua Teng, Sheng Dong, Yanjun Rice (N Y) Original Article BACKGROUND: High temperature affects a broad spectrum of cellular components and metabolism in plants. The Deg/HtrA family of ATP-independent serine endopeptidases is present in nearly all organisms. Deg proteases are required for the survival of Escherichia coli at high temperatures. However, it is still unclear whether rice Deg proteases are required for chloroplast development under high temperatures. RESULTS: In this study, we reported the first rice deg mutant tcm5 (thermo-sensitive chlorophyll-deficient mutant 5) that has an albino phenotype, defective chloroplasts and could not survive after the 4–5 leaf seedling stage when grown at high temperature (32 °C). However, when grown at low temperatures (20 °C), tcm5 has a normal phenotype. Map-based cloning showed that TCM5 encoding a chloroplast-targeted Deg protease protein. The TCM5 transcripts were highly expressed in all green tissues and undetectable in other tissues, showing the tissue-specific expression. In tcm5 mutants grown at high temperatures, the transcript levels of certain genes associated with chloroplast development especially PSII-associated genes were severely affected, but recovered to normal levels at low temperatures. These results showed important role of TCM5 for chloroplast development under high temperatures. CONCLUSIONS: The TCM5 encodes chloroplast-targeted Deg protease protein which is important for chloroplast development and the maintenance of PSII function and its disruption would lead to a defective chloroplast and affected expression levels of genes associated with chloroplast development and photosynthesis at early rice seedling stage under high temperatures. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12284-016-0086-5) contains supplementary material, which is available to authorized users. Springer US 2016-03-21 /pmc/articles/PMC4801845/ /pubmed/27000876 http://dx.doi.org/10.1186/s12284-016-0086-5 Text en © Zheng et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Zheng, Kailun
Zhao, Jian
Lin, Dongzhi
Chen, Jiaying
Xu, Jianlong
Zhou, Hua
Teng, Sheng
Dong, Yanjun
The Rice TCM5 Gene Encoding a Novel Deg Protease Protein is Essential for Chloroplast Development under High Temperatures
title The Rice TCM5 Gene Encoding a Novel Deg Protease Protein is Essential for Chloroplast Development under High Temperatures
title_full The Rice TCM5 Gene Encoding a Novel Deg Protease Protein is Essential for Chloroplast Development under High Temperatures
title_fullStr The Rice TCM5 Gene Encoding a Novel Deg Protease Protein is Essential for Chloroplast Development under High Temperatures
title_full_unstemmed The Rice TCM5 Gene Encoding a Novel Deg Protease Protein is Essential for Chloroplast Development under High Temperatures
title_short The Rice TCM5 Gene Encoding a Novel Deg Protease Protein is Essential for Chloroplast Development under High Temperatures
title_sort rice tcm5 gene encoding a novel deg protease protein is essential for chloroplast development under high temperatures
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4801845/
https://www.ncbi.nlm.nih.gov/pubmed/27000876
http://dx.doi.org/10.1186/s12284-016-0086-5
work_keys_str_mv AT zhengkailun thericetcm5geneencodinganoveldegproteaseproteinisessentialforchloroplastdevelopmentunderhightemperatures
AT zhaojian thericetcm5geneencodinganoveldegproteaseproteinisessentialforchloroplastdevelopmentunderhightemperatures
AT lindongzhi thericetcm5geneencodinganoveldegproteaseproteinisessentialforchloroplastdevelopmentunderhightemperatures
AT chenjiaying thericetcm5geneencodinganoveldegproteaseproteinisessentialforchloroplastdevelopmentunderhightemperatures
AT xujianlong thericetcm5geneencodinganoveldegproteaseproteinisessentialforchloroplastdevelopmentunderhightemperatures
AT zhouhua thericetcm5geneencodinganoveldegproteaseproteinisessentialforchloroplastdevelopmentunderhightemperatures
AT tengsheng thericetcm5geneencodinganoveldegproteaseproteinisessentialforchloroplastdevelopmentunderhightemperatures
AT dongyanjun thericetcm5geneencodinganoveldegproteaseproteinisessentialforchloroplastdevelopmentunderhightemperatures
AT zhengkailun ricetcm5geneencodinganoveldegproteaseproteinisessentialforchloroplastdevelopmentunderhightemperatures
AT zhaojian ricetcm5geneencodinganoveldegproteaseproteinisessentialforchloroplastdevelopmentunderhightemperatures
AT lindongzhi ricetcm5geneencodinganoveldegproteaseproteinisessentialforchloroplastdevelopmentunderhightemperatures
AT chenjiaying ricetcm5geneencodinganoveldegproteaseproteinisessentialforchloroplastdevelopmentunderhightemperatures
AT xujianlong ricetcm5geneencodinganoveldegproteaseproteinisessentialforchloroplastdevelopmentunderhightemperatures
AT zhouhua ricetcm5geneencodinganoveldegproteaseproteinisessentialforchloroplastdevelopmentunderhightemperatures
AT tengsheng ricetcm5geneencodinganoveldegproteaseproteinisessentialforchloroplastdevelopmentunderhightemperatures
AT dongyanjun ricetcm5geneencodinganoveldegproteaseproteinisessentialforchloroplastdevelopmentunderhightemperatures